Confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo.

Early detection and evaluation of brain tumors during surgery is crucial for accurate resection. Currently cryosections during surgery are regularly performed. Confocal laser endomicroscopy (CLE) is a novel technique permitting in vivo histologic imaging with miniaturized endoscopic probes at excell...

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Autores principales:	Sebastian Foersch, Axel Heimann, Ali Ayyad, Gilles A Spoden, Luise Florin, Konstantin Mpoukouvalas, Ralf Kiesslich, Oliver Kempski, Martin Goetz, Patra Charalampaki
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7c5d3efcd3c0450a82a93a508032dd36
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spelling	oai:doaj.org-article:7c5d3efcd3c0450a82a93a508032dd362021-11-18T07:11:16ZConfocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo.1932-620310.1371/journal.pone.0041760https://doaj.org/article/7c5d3efcd3c0450a82a93a508032dd362012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22911853/?tool=EBIhttps://doaj.org/toc/1932-6203Early detection and evaluation of brain tumors during surgery is crucial for accurate resection. Currently cryosections during surgery are regularly performed. Confocal laser endomicroscopy (CLE) is a novel technique permitting in vivo histologic imaging with miniaturized endoscopic probes at excellent resolution. Aim of the current study was to evaluate CLE for in vivo diagnosis in different types and models of intracranial neoplasia. In vivo histomorphology of healthy brains and two different C6 glioma cell line allografts was evaluated in rats. One cell line expressed EYFP, the other cell line was used for staining with fluorescent dyes (fluorescein, acriflavine, FITC-dextran and Indocyanine green). To evaluate future application in patients, fresh surgical resection specimen of human intracranial tumors (n = 15) were examined (glioblastoma multiforme, meningioma, craniopharyngioma, acoustic neurinoma, brain metastasis, medulloblastoma, epidermoid tumor). Healthy brain tissue adjacent to the samples served as control. CLE yielded high-quality histomorphology of normal brain tissue and tumors. Different fluorescent agents revealed distinct aspects of tissue and cell structure (nuclear pattern, axonal pathways, hemorrhages). CLE discrimination of neoplastic from healthy brain tissue was easy to perform based on tissue and cellular architecture and resemblance with histopathology was excellent. Confocal laser endomicroscopy allows immediate in vivo imaging of normal and neoplastic brain tissue at high resolution. The technology might be transferred to scientific and clinical application in neurosurgery and neuropathology. It may become helpful to screen for tumor free margins and to improve the surgical resection of malignant brain tumors, and opens the door to in vivo molecular imaging of tumors and other neurologic disorders.Sebastian FoerschAxel HeimannAli AyyadGilles A SpodenLuise FlorinKonstantin MpoukouvalasRalf KiesslichOliver KempskiMartin GoetzPatra CharalampakiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e41760 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sebastian Foersch Axel Heimann Ali Ayyad Gilles A Spoden Luise Florin Konstantin Mpoukouvalas Ralf Kiesslich Oliver Kempski Martin Goetz Patra Charalampaki Confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo.
description	Early detection and evaluation of brain tumors during surgery is crucial for accurate resection. Currently cryosections during surgery are regularly performed. Confocal laser endomicroscopy (CLE) is a novel technique permitting in vivo histologic imaging with miniaturized endoscopic probes at excellent resolution. Aim of the current study was to evaluate CLE for in vivo diagnosis in different types and models of intracranial neoplasia. In vivo histomorphology of healthy brains and two different C6 glioma cell line allografts was evaluated in rats. One cell line expressed EYFP, the other cell line was used for staining with fluorescent dyes (fluorescein, acriflavine, FITC-dextran and Indocyanine green). To evaluate future application in patients, fresh surgical resection specimen of human intracranial tumors (n = 15) were examined (glioblastoma multiforme, meningioma, craniopharyngioma, acoustic neurinoma, brain metastasis, medulloblastoma, epidermoid tumor). Healthy brain tissue adjacent to the samples served as control. CLE yielded high-quality histomorphology of normal brain tissue and tumors. Different fluorescent agents revealed distinct aspects of tissue and cell structure (nuclear pattern, axonal pathways, hemorrhages). CLE discrimination of neoplastic from healthy brain tissue was easy to perform based on tissue and cellular architecture and resemblance with histopathology was excellent. Confocal laser endomicroscopy allows immediate in vivo imaging of normal and neoplastic brain tissue at high resolution. The technology might be transferred to scientific and clinical application in neurosurgery and neuropathology. It may become helpful to screen for tumor free margins and to improve the surgical resection of malignant brain tumors, and opens the door to in vivo molecular imaging of tumors and other neurologic disorders.
format	article
author	Sebastian Foersch Axel Heimann Ali Ayyad Gilles A Spoden Luise Florin Konstantin Mpoukouvalas Ralf Kiesslich Oliver Kempski Martin Goetz Patra Charalampaki
author_facet	Sebastian Foersch Axel Heimann Ali Ayyad Gilles A Spoden Luise Florin Konstantin Mpoukouvalas Ralf Kiesslich Oliver Kempski Martin Goetz Patra Charalampaki
author_sort	Sebastian Foersch
title	Confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo.
title_short	Confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo.
title_full	Confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo.
title_fullStr	Confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo.
title_full_unstemmed	Confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo.
title_sort	confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/7c5d3efcd3c0450a82a93a508032dd36
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Confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo.

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