In vivo investigation on the chronic hepatotoxicity induced by intraperitoneal administration of 10-nm silicon dioxide nanoparticles

Mansour Almansour,1 Saud Alarifi,1 Bashir Jarrar21Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Department of Biological Sciences, College of Science, Jerash University, Jerash, JordanBackground: Silicon dioxide (silica) nanoparticles (SDNPs) are widely used...

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Autores principales:	Almansour M, Alarifi S, Jarrar B
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	nanotoxicity histological alterations morphometric alterations hematological alterations biochemical alterations gene expression. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7c5e7486748c4ff59b144cf22c6ca938
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spelling	oai:doaj.org-article:7c5e7486748c4ff59b144cf22c6ca9382021-12-02T01:41:52ZIn vivo investigation on the chronic hepatotoxicity induced by intraperitoneal administration of 10-nm silicon dioxide nanoparticles1178-2013https://doaj.org/article/7c5e7486748c4ff59b144cf22c6ca9382018-05-01T00:00:00Zhttps://www.dovepress.com/in-vivo-investigation-on-the-chronic-hepatotoxicity-induced-by-intrape-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Mansour Almansour,1 Saud Alarifi,1 Bashir Jarrar21Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Department of Biological Sciences, College of Science, Jerash University, Jerash, JordanBackground: Silicon dioxide (silica) nanoparticles (SDNPs) are widely used in nanotechnology and medicine, but these nanomaterials may carry a high risk for human health while little is known about their toxicity. Methods: We investigated the alterations in morphometry, biochemistry, hematology, histology of liver tissue and gene expression of drug-metabolizing enzymes induced by 10-nm SDNPs. Healthy male Wistar albino rats were exposed to 20, 35 and 50 repeated injections of SDNPs (2 mg/kg body weight). Whole blood, serum and plasma samples were used for hematological and biochemical analyses, whereas liver biopsies were processed for histopathological and gene expression alterations. Results: In comparison with control rats, exposure to SDNPs lowered the body weight gain and liver index and increased the counts of white blood cells and platelets, but lowered the platelet larger cell ratio and plateletcrit. Levels of alkaline phosphatase, lactate dehydrogenase, low-density lipids, procalcitonin, aspartate aminotransferase and alanine aminotransferase, as well as potassium, phosphorus and iron concentrations, were increased. Histopathology revealed that SDNPs could induce hydropic degeneration, sinusoidal dilatation, hyperplasia of Kupffer cells, karyopyknosis and infiltration of inflammatory cells in the liver. SDNPs reduced the expression of 12 genes of drug-metabolizing enzymes significantly (p<0.05). Conclusion: These results suggest that SDNPs could cause alterations in morphometry, biochemistry, hematology, liver tissues and the expression of drug-metabolizing enzyme genes. Keywords: toxicity, histological alterations, morphometric alterations, hematological alterations, biochemical alterations, gene expressionAlmansour MAlarifi SJarrar BDove Medical Pressarticlenanotoxicityhistological alterationsmorphometric alterationshematological alterationsbiochemical alterationsgene expression.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 2685-2696 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanotoxicity histological alterations morphometric alterations hematological alterations biochemical alterations gene expression. Medicine (General) R5-920
spellingShingle	nanotoxicity histological alterations morphometric alterations hematological alterations biochemical alterations gene expression. Medicine (General) R5-920 Almansour M Alarifi S Jarrar B In vivo investigation on the chronic hepatotoxicity induced by intraperitoneal administration of 10-nm silicon dioxide nanoparticles
description	Mansour Almansour,1 Saud Alarifi,1 Bashir Jarrar21Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Department of Biological Sciences, College of Science, Jerash University, Jerash, JordanBackground: Silicon dioxide (silica) nanoparticles (SDNPs) are widely used in nanotechnology and medicine, but these nanomaterials may carry a high risk for human health while little is known about their toxicity. Methods: We investigated the alterations in morphometry, biochemistry, hematology, histology of liver tissue and gene expression of drug-metabolizing enzymes induced by 10-nm SDNPs. Healthy male Wistar albino rats were exposed to 20, 35 and 50 repeated injections of SDNPs (2 mg/kg body weight). Whole blood, serum and plasma samples were used for hematological and biochemical analyses, whereas liver biopsies were processed for histopathological and gene expression alterations. Results: In comparison with control rats, exposure to SDNPs lowered the body weight gain and liver index and increased the counts of white blood cells and platelets, but lowered the platelet larger cell ratio and plateletcrit. Levels of alkaline phosphatase, lactate dehydrogenase, low-density lipids, procalcitonin, aspartate aminotransferase and alanine aminotransferase, as well as potassium, phosphorus and iron concentrations, were increased. Histopathology revealed that SDNPs could induce hydropic degeneration, sinusoidal dilatation, hyperplasia of Kupffer cells, karyopyknosis and infiltration of inflammatory cells in the liver. SDNPs reduced the expression of 12 genes of drug-metabolizing enzymes significantly (p<0.05). Conclusion: These results suggest that SDNPs could cause alterations in morphometry, biochemistry, hematology, liver tissues and the expression of drug-metabolizing enzyme genes. Keywords: toxicity, histological alterations, morphometric alterations, hematological alterations, biochemical alterations, gene expression
format	article
author	Almansour M Alarifi S Jarrar B
author_facet	Almansour M Alarifi S Jarrar B
author_sort	Almansour M
title	In vivo investigation on the chronic hepatotoxicity induced by intraperitoneal administration of 10-nm silicon dioxide nanoparticles
title_short	In vivo investigation on the chronic hepatotoxicity induced by intraperitoneal administration of 10-nm silicon dioxide nanoparticles
title_full	In vivo investigation on the chronic hepatotoxicity induced by intraperitoneal administration of 10-nm silicon dioxide nanoparticles
title_fullStr	In vivo investigation on the chronic hepatotoxicity induced by intraperitoneal administration of 10-nm silicon dioxide nanoparticles
title_full_unstemmed	In vivo investigation on the chronic hepatotoxicity induced by intraperitoneal administration of 10-nm silicon dioxide nanoparticles
title_sort	in vivo investigation on the chronic hepatotoxicity induced by intraperitoneal administration of 10-nm silicon dioxide nanoparticles
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/7c5e7486748c4ff59b144cf22c6ca938
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In vivo investigation on the chronic hepatotoxicity induced by intraperitoneal administration of 10-nm silicon dioxide nanoparticles

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