Detection of AMA-M2 in human saliva: Potentials in diagnosis and monitoring of primary biliary cholangitis

Abstract Serum anti-mitochondrial antibody type 2 (AMA-M2) is considered as a pivotal biomarker for the diagnosis of primary biliary cholangitis (PBC). However, serological tests have many limitations, including inconvenience, invasiveness, and infection risks. Thus, a less invasive approach to dete...

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Autores principales:	Chong Lu, Xianliang Hou, Minwei Li, Lin Wang, Ping Zeng, Hongyu Jia, Jianing Chen, Yingfeng Wei, Hong He, Xiangdong Liu, Hongyan Diao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7c61ed5d533d4f319933c67df45c71a9
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spelling	oai:doaj.org-article:7c61ed5d533d4f319933c67df45c71a92021-12-02T12:32:43ZDetection of AMA-M2 in human saliva: Potentials in diagnosis and monitoring of primary biliary cholangitis10.1038/s41598-017-00906-12045-2322https://doaj.org/article/7c61ed5d533d4f319933c67df45c71a92017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00906-1https://doaj.org/toc/2045-2322Abstract Serum anti-mitochondrial antibody type 2 (AMA-M2) is considered as a pivotal biomarker for the diagnosis of primary biliary cholangitis (PBC). However, serological tests have many limitations, including inconvenience, invasiveness, and infection risks. Thus, a less invasive approach to detect AMA-M2 titer is desirable. We examined salivary AMA-M2 of potential PBC patients and found that AMA-M2 could be detected only in saliva of serum AMA-M2-positive PBC patients, but not in saliva of serum AMA-M2-negative PBC patients, oral lichen planus patients (OLP) patients, or healthy controls. Furthermore, the concentration of salivary AMA-M2 was positively correlated with the amount of serum AMA-M2 in patients. The salivary inflammatory cytokines were increased in the PBC, consistent with the results of serum test. These findings indicated that saliva might be a less invasive and cost-effective medium to accurately test for AMA-M2 levels and this is a promising development for the diagnosis and monitoring of PBC.Chong LuXianliang HouMinwei LiLin WangPing ZengHongyu JiaJianing ChenYingfeng WeiHong HeXiangdong LiuHongyan DiaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chong Lu Xianliang Hou Minwei Li Lin Wang Ping Zeng Hongyu Jia Jianing Chen Yingfeng Wei Hong He Xiangdong Liu Hongyan Diao Detection of AMA-M2 in human saliva: Potentials in diagnosis and monitoring of primary biliary cholangitis
description	Abstract Serum anti-mitochondrial antibody type 2 (AMA-M2) is considered as a pivotal biomarker for the diagnosis of primary biliary cholangitis (PBC). However, serological tests have many limitations, including inconvenience, invasiveness, and infection risks. Thus, a less invasive approach to detect AMA-M2 titer is desirable. We examined salivary AMA-M2 of potential PBC patients and found that AMA-M2 could be detected only in saliva of serum AMA-M2-positive PBC patients, but not in saliva of serum AMA-M2-negative PBC patients, oral lichen planus patients (OLP) patients, or healthy controls. Furthermore, the concentration of salivary AMA-M2 was positively correlated with the amount of serum AMA-M2 in patients. The salivary inflammatory cytokines were increased in the PBC, consistent with the results of serum test. These findings indicated that saliva might be a less invasive and cost-effective medium to accurately test for AMA-M2 levels and this is a promising development for the diagnosis and monitoring of PBC.
format	article
author	Chong Lu Xianliang Hou Minwei Li Lin Wang Ping Zeng Hongyu Jia Jianing Chen Yingfeng Wei Hong He Xiangdong Liu Hongyan Diao
author_facet	Chong Lu Xianliang Hou Minwei Li Lin Wang Ping Zeng Hongyu Jia Jianing Chen Yingfeng Wei Hong He Xiangdong Liu Hongyan Diao
author_sort	Chong Lu
title	Detection of AMA-M2 in human saliva: Potentials in diagnosis and monitoring of primary biliary cholangitis
title_short	Detection of AMA-M2 in human saliva: Potentials in diagnosis and monitoring of primary biliary cholangitis
title_full	Detection of AMA-M2 in human saliva: Potentials in diagnosis and monitoring of primary biliary cholangitis
title_fullStr	Detection of AMA-M2 in human saliva: Potentials in diagnosis and monitoring of primary biliary cholangitis
title_full_unstemmed	Detection of AMA-M2 in human saliva: Potentials in diagnosis and monitoring of primary biliary cholangitis
title_sort	detection of ama-m2 in human saliva: potentials in diagnosis and monitoring of primary biliary cholangitis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7c61ed5d533d4f319933c67df45c71a9
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Detection of AMA-M2 in human saliva: Potentials in diagnosis and monitoring of primary biliary cholangitis

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