Towards establishment of a rice stress response interactome.
Rice (Oryza sativa) is a staple food for more than half the world and a model for studies of monocotyledonous species, which include cereal crops and candidate bioenergy grasses. A major limitation of crop production is imposed by a suite of abiotic and biotic stresses resulting in 30%-60% yield los...
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Public Library of Science (PLoS)
2011
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oai:doaj.org-article:7c699b27286a48fc8aafe9628ded620c2021-11-18T06:17:33ZTowards establishment of a rice stress response interactome.1553-73901553-740410.1371/journal.pgen.1002020https://doaj.org/article/7c699b27286a48fc8aafe9628ded620c2011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21533176/?tool=EBIhttps://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Rice (Oryza sativa) is a staple food for more than half the world and a model for studies of monocotyledonous species, which include cereal crops and candidate bioenergy grasses. A major limitation of crop production is imposed by a suite of abiotic and biotic stresses resulting in 30%-60% yield losses globally each year. To elucidate stress response signaling networks, we constructed an interactome of 100 proteins by yeast two-hybrid (Y2H) assays around key regulators of the rice biotic and abiotic stress responses. We validated the interactome using protein-protein interaction (PPI) assays, co-expression of transcripts, and phenotypic analyses. Using this interactome-guided prediction and phenotype validation, we identified ten novel regulators of stress tolerance, including two from protein classes not previously known to function in stress responses. Several lines of evidence support cross-talk between biotic and abiotic stress responses. The combination of focused interactome and systems analyses described here represents significant progress toward elucidating the molecular basis of traits of agronomic importance.Young-Su SeoMawsheng ChernLaura E BartleyMuho HanKi-Hong JungInsuk LeeHarkamal WaliaTodd RichterXia XuPeijian CaoWei BaiRajeshwari RamananFawn AmonpantLoganathan ArulPatrick E CanlasRandy RuanChang-Jin ParkXuewei ChenSohyun HwangJong-Seong JeonPamela C RonaldPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 7, Iss 4, p e1002020 (2011) |
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Genetics QH426-470 |
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Genetics QH426-470 Young-Su Seo Mawsheng Chern Laura E Bartley Muho Han Ki-Hong Jung Insuk Lee Harkamal Walia Todd Richter Xia Xu Peijian Cao Wei Bai Rajeshwari Ramanan Fawn Amonpant Loganathan Arul Patrick E Canlas Randy Ruan Chang-Jin Park Xuewei Chen Sohyun Hwang Jong-Seong Jeon Pamela C Ronald Towards establishment of a rice stress response interactome. |
| description |
Rice (Oryza sativa) is a staple food for more than half the world and a model for studies of monocotyledonous species, which include cereal crops and candidate bioenergy grasses. A major limitation of crop production is imposed by a suite of abiotic and biotic stresses resulting in 30%-60% yield losses globally each year. To elucidate stress response signaling networks, we constructed an interactome of 100 proteins by yeast two-hybrid (Y2H) assays around key regulators of the rice biotic and abiotic stress responses. We validated the interactome using protein-protein interaction (PPI) assays, co-expression of transcripts, and phenotypic analyses. Using this interactome-guided prediction and phenotype validation, we identified ten novel regulators of stress tolerance, including two from protein classes not previously known to function in stress responses. Several lines of evidence support cross-talk between biotic and abiotic stress responses. The combination of focused interactome and systems analyses described here represents significant progress toward elucidating the molecular basis of traits of agronomic importance. |
| format |
article |
| author |
Young-Su Seo Mawsheng Chern Laura E Bartley Muho Han Ki-Hong Jung Insuk Lee Harkamal Walia Todd Richter Xia Xu Peijian Cao Wei Bai Rajeshwari Ramanan Fawn Amonpant Loganathan Arul Patrick E Canlas Randy Ruan Chang-Jin Park Xuewei Chen Sohyun Hwang Jong-Seong Jeon Pamela C Ronald |
| author_facet |
Young-Su Seo Mawsheng Chern Laura E Bartley Muho Han Ki-Hong Jung Insuk Lee Harkamal Walia Todd Richter Xia Xu Peijian Cao Wei Bai Rajeshwari Ramanan Fawn Amonpant Loganathan Arul Patrick E Canlas Randy Ruan Chang-Jin Park Xuewei Chen Sohyun Hwang Jong-Seong Jeon Pamela C Ronald |
| author_sort |
Young-Su Seo |
| title |
Towards establishment of a rice stress response interactome. |
| title_short |
Towards establishment of a rice stress response interactome. |
| title_full |
Towards establishment of a rice stress response interactome. |
| title_fullStr |
Towards establishment of a rice stress response interactome. |
| title_full_unstemmed |
Towards establishment of a rice stress response interactome. |
| title_sort |
towards establishment of a rice stress response interactome. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/7c699b27286a48fc8aafe9628ded620c |
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