Nanocapsules Containing Neem (Azadirachta Indica) Oil: Development, Characterization, And Toxicity Evaluation

Abstract In this study, we prepared, characterized, and performed toxicity analyses of poly(ε-caprolactone) nanocapsules loaded with neem oil. Three formulations were prepared by the emulsion/solvent evaporation method. The nanocapsules showed a mean size distribution around 400 nm, with polydispers...

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Autores principales:	Tatiane Pasquoto-Stigliani, Estefânia V. R. Campos, Jhones L. Oliveira, Camila M. G. Silva, Natalia Bilesky-José, Mariana Guilger, Johann Troost, Halley C. Oliveira, Renata Stolf-Moreira, Leonardo F. Fraceto, Renata de Lima
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7c705edfd54b46bbb1693cbe3465b5b3
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spelling	oai:doaj.org-article:7c705edfd54b46bbb1693cbe3465b5b32021-12-02T15:06:18ZNanocapsules Containing Neem (Azadirachta Indica) Oil: Development, Characterization, And Toxicity Evaluation10.1038/s41598-017-06092-42045-2322https://doaj.org/article/7c705edfd54b46bbb1693cbe3465b5b32017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06092-4https://doaj.org/toc/2045-2322Abstract In this study, we prepared, characterized, and performed toxicity analyses of poly(ε-caprolactone) nanocapsules loaded with neem oil. Three formulations were prepared by the emulsion/solvent evaporation method. The nanocapsules showed a mean size distribution around 400 nm, with polydispersity below 0.2 and were stable for 120 days. Cytotoxicity and genotoxicity results showed an increase in toxicity of the oleic acid + neem formulations according to the amount of oleic acid used. The minimum inhibitory concentrations demonstrated that all the formulations containing neem oil were active. The nanocapsules containing neem oil did not affect the soil microbiota during 300 days of exposure compared to the control. Phytotoxicity studies indicated that NC_20 (200 mg of neem oil) did not affect the net photosynthesis and stomatal conductance of maize plants, whereas use of NC_10 (100:100 of neem:oleic acid) and NC_15 (150:50 of neem:oleic acid) led to negative effects on these physiological parameters. Hence, the use of oleic acid as a complement in the nanocapsules was not a good strategy, since the nanocapsules that only contained neem oil showed lower toxicity. These results demonstrate that evaluation of the toxicity of nanopesticides is essential for the development of environmentally friendly formulations intended for applications in agriculture.Tatiane Pasquoto-StiglianiEstefânia V. R. CamposJhones L. OliveiraCamila M. G. SilvaNatalia Bilesky-JoséMariana GuilgerJohann TroostHalley C. OliveiraRenata Stolf-MoreiraLeonardo F. FracetoRenata de LimaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tatiane Pasquoto-Stigliani Estefânia V. R. Campos Jhones L. Oliveira Camila M. G. Silva Natalia Bilesky-José Mariana Guilger Johann Troost Halley C. Oliveira Renata Stolf-Moreira Leonardo F. Fraceto Renata de Lima Nanocapsules Containing Neem (Azadirachta Indica) Oil: Development, Characterization, And Toxicity Evaluation
description	Abstract In this study, we prepared, characterized, and performed toxicity analyses of poly(ε-caprolactone) nanocapsules loaded with neem oil. Three formulations were prepared by the emulsion/solvent evaporation method. The nanocapsules showed a mean size distribution around 400 nm, with polydispersity below 0.2 and were stable for 120 days. Cytotoxicity and genotoxicity results showed an increase in toxicity of the oleic acid + neem formulations according to the amount of oleic acid used. The minimum inhibitory concentrations demonstrated that all the formulations containing neem oil were active. The nanocapsules containing neem oil did not affect the soil microbiota during 300 days of exposure compared to the control. Phytotoxicity studies indicated that NC_20 (200 mg of neem oil) did not affect the net photosynthesis and stomatal conductance of maize plants, whereas use of NC_10 (100:100 of neem:oleic acid) and NC_15 (150:50 of neem:oleic acid) led to negative effects on these physiological parameters. Hence, the use of oleic acid as a complement in the nanocapsules was not a good strategy, since the nanocapsules that only contained neem oil showed lower toxicity. These results demonstrate that evaluation of the toxicity of nanopesticides is essential for the development of environmentally friendly formulations intended for applications in agriculture.
format	article
author	Tatiane Pasquoto-Stigliani Estefânia V. R. Campos Jhones L. Oliveira Camila M. G. Silva Natalia Bilesky-José Mariana Guilger Johann Troost Halley C. Oliveira Renata Stolf-Moreira Leonardo F. Fraceto Renata de Lima
author_facet	Tatiane Pasquoto-Stigliani Estefânia V. R. Campos Jhones L. Oliveira Camila M. G. Silva Natalia Bilesky-José Mariana Guilger Johann Troost Halley C. Oliveira Renata Stolf-Moreira Leonardo F. Fraceto Renata de Lima
author_sort	Tatiane Pasquoto-Stigliani
title	Nanocapsules Containing Neem (Azadirachta Indica) Oil: Development, Characterization, And Toxicity Evaluation
title_short	Nanocapsules Containing Neem (Azadirachta Indica) Oil: Development, Characterization, And Toxicity Evaluation
title_full	Nanocapsules Containing Neem (Azadirachta Indica) Oil: Development, Characterization, And Toxicity Evaluation
title_fullStr	Nanocapsules Containing Neem (Azadirachta Indica) Oil: Development, Characterization, And Toxicity Evaluation
title_full_unstemmed	Nanocapsules Containing Neem (Azadirachta Indica) Oil: Development, Characterization, And Toxicity Evaluation
title_sort	nanocapsules containing neem (azadirachta indica) oil: development, characterization, and toxicity evaluation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7c705edfd54b46bbb1693cbe3465b5b3
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Nanocapsules Containing Neem (Azadirachta Indica) Oil: Development, Characterization, And Toxicity Evaluation

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