A heparan-sulfate-bearing syndecan-1 glycoform is a distinct surface marker for intra-tumoral myeloid-derived suppressor cells

Summary: Myeloid-derived suppressor cells (MDSCs) infiltrate cancer tissue, promote tumor growth, and are associated with resistance to cancer therapies. However, there is no practical approach available to distinguish MDSCs from mature counterparts inside tumors. Here, we show that a recently isola...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Thomas Welte, Junhua Mai, Zhe Zhang, Shaohui Tian, Guodong Zhang, Yitian Xu, Licheng Zhang, Shu-shia Chen, Tian Wang, Haifa Shen
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Molecular biology Immunology Cell biology Science Q
Acceso en línea:	https://doaj.org/article/7c73f5260e8348c5902ad6ec129b4de4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7c73f5260e8348c5902ad6ec129b4de4
record_format	dspace
spelling	oai:doaj.org-article:7c73f5260e8348c5902ad6ec129b4de42021-11-20T05:10:21ZA heparan-sulfate-bearing syndecan-1 glycoform is a distinct surface marker for intra-tumoral myeloid-derived suppressor cells2589-004210.1016/j.isci.2021.103349https://doaj.org/article/7c73f5260e8348c5902ad6ec129b4de42021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221013183https://doaj.org/toc/2589-0042Summary: Myeloid-derived suppressor cells (MDSCs) infiltrate cancer tissue, promote tumor growth, and are associated with resistance to cancer therapies. However, there is no practical approach available to distinguish MDSCs from mature counterparts inside tumors. Here, we show that a recently isolated thioaptamer probe (T1) binds to MDSC subsets in colorectal and pancreatic tumors with high specificity. Whole transcriptome and functional analysis revealed that T1-binding cells contain polymorphonuclear (PMN)-MDSCs characterized by several immunosuppression pathways, ROS production, and T cell suppression activity, whereas T1-non-binding PMNs were mature and nonsuppressive. We identified syndecan-1 as the T1-interacting protein on MDSCs and chronic myelogenous leukemia K562 cell line. Heparan sulfate chains were essential in T1-binding. Inside tumors PMN-MDSCs expressed heparan sulfate biogenesis enzymes at higher levels. Tumor-cell-derived soluble factor(s) enhanced MDSCs' affinity for T1. Overall, we uncovered heparan-sulfate-dependent MDSC modulation in the tumor microenvironment and identified T1 as tool preferentially targeting tumor-promoting myeloid cell subsets.Thomas WelteJunhua MaiZhe ZhangShaohui TianGuodong ZhangYitian XuLicheng ZhangShu-shia ChenTian WangHaifa ShenElsevierarticleMolecular biologyImmunologyCell biologyScienceQENiScience, Vol 24, Iss 11, Pp 103349- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Molecular biology Immunology Cell biology Science Q
spellingShingle	Molecular biology Immunology Cell biology Science Q Thomas Welte Junhua Mai Zhe Zhang Shaohui Tian Guodong Zhang Yitian Xu Licheng Zhang Shu-shia Chen Tian Wang Haifa Shen A heparan-sulfate-bearing syndecan-1 glycoform is a distinct surface marker for intra-tumoral myeloid-derived suppressor cells
description	Summary: Myeloid-derived suppressor cells (MDSCs) infiltrate cancer tissue, promote tumor growth, and are associated with resistance to cancer therapies. However, there is no practical approach available to distinguish MDSCs from mature counterparts inside tumors. Here, we show that a recently isolated thioaptamer probe (T1) binds to MDSC subsets in colorectal and pancreatic tumors with high specificity. Whole transcriptome and functional analysis revealed that T1-binding cells contain polymorphonuclear (PMN)-MDSCs characterized by several immunosuppression pathways, ROS production, and T cell suppression activity, whereas T1-non-binding PMNs were mature and nonsuppressive. We identified syndecan-1 as the T1-interacting protein on MDSCs and chronic myelogenous leukemia K562 cell line. Heparan sulfate chains were essential in T1-binding. Inside tumors PMN-MDSCs expressed heparan sulfate biogenesis enzymes at higher levels. Tumor-cell-derived soluble factor(s) enhanced MDSCs' affinity for T1. Overall, we uncovered heparan-sulfate-dependent MDSC modulation in the tumor microenvironment and identified T1 as tool preferentially targeting tumor-promoting myeloid cell subsets.
format	article
author	Thomas Welte Junhua Mai Zhe Zhang Shaohui Tian Guodong Zhang Yitian Xu Licheng Zhang Shu-shia Chen Tian Wang Haifa Shen
author_facet	Thomas Welte Junhua Mai Zhe Zhang Shaohui Tian Guodong Zhang Yitian Xu Licheng Zhang Shu-shia Chen Tian Wang Haifa Shen
author_sort	Thomas Welte
title	A heparan-sulfate-bearing syndecan-1 glycoform is a distinct surface marker for intra-tumoral myeloid-derived suppressor cells
title_short	A heparan-sulfate-bearing syndecan-1 glycoform is a distinct surface marker for intra-tumoral myeloid-derived suppressor cells
title_full	A heparan-sulfate-bearing syndecan-1 glycoform is a distinct surface marker for intra-tumoral myeloid-derived suppressor cells
title_fullStr	A heparan-sulfate-bearing syndecan-1 glycoform is a distinct surface marker for intra-tumoral myeloid-derived suppressor cells
title_full_unstemmed	A heparan-sulfate-bearing syndecan-1 glycoform is a distinct surface marker for intra-tumoral myeloid-derived suppressor cells
title_sort	heparan-sulfate-bearing syndecan-1 glycoform is a distinct surface marker for intra-tumoral myeloid-derived suppressor cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/7c73f5260e8348c5902ad6ec129b4de4
work_keys_str_mv	AT thomaswelte aheparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT junhuamai aheparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT zhezhang aheparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT shaohuitian aheparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT guodongzhang aheparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT yitianxu aheparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT lichengzhang aheparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT shushiachen aheparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT tianwang aheparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT haifashen aheparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT thomaswelte heparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT junhuamai heparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT zhezhang heparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT shaohuitian heparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT guodongzhang heparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT yitianxu heparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT lichengzhang heparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT shushiachen heparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT tianwang heparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells AT haifashen heparansulfatebearingsyndecan1glycoformisadistinctsurfacemarkerforintratumoralmyeloidderivedsuppressorcells
_version_	1718419549492084736

A heparan-sulfate-bearing syndecan-1 glycoform is a distinct surface marker for intra-tumoral myeloid-derived suppressor cells

Ejemplares similares