Pretreatment with intraluminal rapamycin nanoparticle perfusion inhibits neointimal hyperplasia in a rabbit vein graft model

Kai Liu1*, Guangqing Cao1*, Xiquan Zhang1, Ruifang Liu2, Weiwei Zou3, Shuming Wu11Departments of Cardiovascular Surgery, 2Anesthesia, Qilu Hospital of Shandong University, Jinan; 3The School of Pharmaceutical Science, Shandong University, Jinan, Shandong, People&rsquo;s Republic of China; *K...

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Autores principales:	Kai Liu, Guangqing Cao, Xiquan Zhang, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7c776c8debc742f1aabafa7011dc1455
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spelling	oai:doaj.org-article:7c776c8debc742f1aabafa7011dc14552021-12-02T02:27:10ZPretreatment with intraluminal rapamycin nanoparticle perfusion inhibits neointimal hyperplasia in a rabbit vein graft model1176-91141178-2013https://doaj.org/article/7c776c8debc742f1aabafa7011dc14552010-10-01T00:00:00Zhttp://www.dovepress.com/pretreatment-with-intraluminal-rapamycin-nanoparticle-perfusion-inhibi-a5481https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Kai Liu1, Guangqing Cao1, Xiquan Zhang1, Ruifang Liu2, Weiwei Zou3, Shuming Wu11Departments of Cardiovascular Surgery, 2Anesthesia, Qilu Hospital of Shandong University, Jinan; 3The School of Pharmaceutical Science, Shandong University, Jinan, Shandong, People&rsquo;s Republic of China; *Kai Liu and Guangqing Cao contributed equally to this work.Purpose: Poly lactic-co-glycolic acid nanoparticles (PLGA-NP) are widely used as a biodegradable biomaterial in medicine. Rapamycin-eluting stents have been used for prevention of restenosis during surgery. This study investigated the effect of pretreatment with intraluminal perfusion of carbopol-encapsulated rapamycin-loaded PLGA nanoparticles (RAP-PLGA-NP) on neointimal hyperplasia in a rabbit vein graft model.Methods: A segment of common carotid artery was replaced with a segment of external jugular vein in 60 rabbits which were then separated into four treatment groups, ie, Group 1, in which vein grafts were pretreated with intraluminal RAP-PLGA-NP perfusion, Group 2 in which vein grafts underwent equivalent empty vehicle (PLGA-NP) perfusion, Group 3, in which vein grafts received no treatment, and Group 4, which served as a sham operation group receiving normal vein contrast. On postoperative day 28, the grafts and normal veins were harvested for histologic examination, flow cytometry analysis, and high-performance liquid chromatography measurement.Results: Compared with Group 1, the intima of the grafts were thickened, the ratio of intimal area to vessel area increased, and the collagen volume index of the vein grafts increased significantly in Groups 2 and 3. The cell proliferation index in Group 1 (21.11 &plusmn; 3.15%) was much lower than that in Group 2 (30.35 &plusmn; 2.69%) and in Group 3 (33.86 &plusmn; 8.72%). By high-performance liquid chromatography measurement, retention of rapamycin was detected in Group 1 (11.2 &plusmn; 0.37 &micro;g/10 mg) 28 days after single drug perfusion.Conclusion: Pretreatment with intraluminal RAP-PLGA-NP perfusion may inhibit neointimal hyperplasia in vein grafts by penetrating into local tissue and limiting cell proliferation.Keywords: autograft, stenosis, jugular vein, transplants Kai LiuGuangqing CaoXiquan Zhanget alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 853-860 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Kai Liu Guangqing Cao Xiquan Zhang et al Pretreatment with intraluminal rapamycin nanoparticle perfusion inhibits neointimal hyperplasia in a rabbit vein graft model
description	Kai Liu1, Guangqing Cao1, Xiquan Zhang1, Ruifang Liu2, Weiwei Zou3, Shuming Wu11Departments of Cardiovascular Surgery, 2Anesthesia, Qilu Hospital of Shandong University, Jinan; 3The School of Pharmaceutical Science, Shandong University, Jinan, Shandong, People&rsquo;s Republic of China; *Kai Liu and Guangqing Cao contributed equally to this work.Purpose: Poly lactic-co-glycolic acid nanoparticles (PLGA-NP) are widely used as a biodegradable biomaterial in medicine. Rapamycin-eluting stents have been used for prevention of restenosis during surgery. This study investigated the effect of pretreatment with intraluminal perfusion of carbopol-encapsulated rapamycin-loaded PLGA nanoparticles (RAP-PLGA-NP) on neointimal hyperplasia in a rabbit vein graft model.Methods: A segment of common carotid artery was replaced with a segment of external jugular vein in 60 rabbits which were then separated into four treatment groups, ie, Group 1, in which vein grafts were pretreated with intraluminal RAP-PLGA-NP perfusion, Group 2 in which vein grafts underwent equivalent empty vehicle (PLGA-NP) perfusion, Group 3, in which vein grafts received no treatment, and Group 4, which served as a sham operation group receiving normal vein contrast. On postoperative day 28, the grafts and normal veins were harvested for histologic examination, flow cytometry analysis, and high-performance liquid chromatography measurement.Results: Compared with Group 1, the intima of the grafts were thickened, the ratio of intimal area to vessel area increased, and the collagen volume index of the vein grafts increased significantly in Groups 2 and 3. The cell proliferation index in Group 1 (21.11 &plusmn; 3.15%) was much lower than that in Group 2 (30.35 &plusmn; 2.69%) and in Group 3 (33.86 &plusmn; 8.72%). By high-performance liquid chromatography measurement, retention of rapamycin was detected in Group 1 (11.2 &plusmn; 0.37 &micro;g/10 mg) 28 days after single drug perfusion.Conclusion: Pretreatment with intraluminal RAP-PLGA-NP perfusion may inhibit neointimal hyperplasia in vein grafts by penetrating into local tissue and limiting cell proliferation.Keywords: autograft, stenosis, jugular vein, transplants
format	article
author	Kai Liu Guangqing Cao Xiquan Zhang et al
author_facet	Kai Liu Guangqing Cao Xiquan Zhang et al
author_sort	Kai Liu
title	Pretreatment with intraluminal rapamycin nanoparticle perfusion inhibits neointimal hyperplasia in a rabbit vein graft model
title_short	Pretreatment with intraluminal rapamycin nanoparticle perfusion inhibits neointimal hyperplasia in a rabbit vein graft model
title_full	Pretreatment with intraluminal rapamycin nanoparticle perfusion inhibits neointimal hyperplasia in a rabbit vein graft model
title_fullStr	Pretreatment with intraluminal rapamycin nanoparticle perfusion inhibits neointimal hyperplasia in a rabbit vein graft model
title_full_unstemmed	Pretreatment with intraluminal rapamycin nanoparticle perfusion inhibits neointimal hyperplasia in a rabbit vein graft model
title_sort	pretreatment with intraluminal rapamycin nanoparticle perfusion inhibits neointimal hyperplasia in a rabbit vein graft model
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/7c776c8debc742f1aabafa7011dc1455
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Pretreatment with intraluminal rapamycin nanoparticle perfusion inhibits neointimal hyperplasia in a rabbit vein graft model

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