MicroRNA-151a-3p Functions in the Regulation of Osteoclast Differentiation: Significance to Postmenopausal Osteoporosis

Yuehui He, Di Chen, Qian Guo, Pinghua Shi, Conglei You, Yanping Feng Community Medicine Department, Beijing Jishuitan Hospital, Beijing City, 100096, People’s Republic of ChinaCorrespondence: Di ChenCommunity Medicine Department, Beijing Jishuitan Hospital, No. 68, Huinanbei Road, Huilongguan Town,...

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Autores principales:	He Y, Chen D, Guo Q, Shi P, You C, Feng Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	mirna-151a-3p postmenopausal osteoporosis osteoclast differentiation raw264.7 cells thp-1 cells Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/7c7c87cd3de945ae9fa022ac34cdc227
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spelling	oai:doaj.org-article:7c7c87cd3de945ae9fa022ac34cdc2272021-12-02T16:11:44ZMicroRNA-151a-3p Functions in the Regulation of Osteoclast Differentiation: Significance to Postmenopausal Osteoporosis1178-1998https://doaj.org/article/7c7c87cd3de945ae9fa022ac34cdc2272021-07-01T00:00:00Zhttps://www.dovepress.com/microrna-151a-3p-functions-in-the-regulation-of-osteoclast-differentia-peer-reviewed-fulltext-article-CIAhttps://doaj.org/toc/1178-1998Yuehui He, Di Chen, Qian Guo, Pinghua Shi, Conglei You, Yanping Feng Community Medicine Department, Beijing Jishuitan Hospital, Beijing City, 100096, People’s Republic of ChinaCorrespondence: Di ChenCommunity Medicine Department, Beijing Jishuitan Hospital, No. 68, Huinanbei Road, Huilongguan Town, Changping District, Beijing City, 100096, People’s Republic of ChinaEmail zr4560@163.comBackground: Studies have found the pivotal role of miRNAs in the progression of postmenopausal osteoporosis (OP). However, the function of miRNAs in OP is unclear. This study aimed to explore the biological functions of microRNA-151a-3p in OP.Methods: RT-qPCR was employed to assess the expression of microRNA-151a-3p in serum isolated from OP patients and healthy controls. Dual-energy X-ray absorptiometry (DXA) was used to measure the bone mineral density (BMD) of the lumbar spine. The expression levels of c-Fos, NFATc1, and TRAP were tested by Western blot. Ovariectomized (OVX) rats were treated with antago microRNA-151a-3p or antago NC, and then serum and lumbar vertebrae were collected for ELISA and bone histomorphology analysis.Results: The expression of microRNA-151a-3p in postmenopausal women with osteoporosis was significantly up-regulated, and microRNA-151a-3p level was negatively correlated with BMD. During osteoclastogenesis, microRNA-151a-3p level was obviously increased. Overexpression of microRNA-151a-3p promoted the differentiation of RANKL-induced THP-1 and RAW264.7 cells into osteoclasts, whereas silencing of microRNA-151a-3p resulted in the opposite results. Silencing of microRNA-151a-3p in OVX rats altered osteoclastogenesis-related factors and raised BMD.Conclusion: MicroRNA-151a-3p could partly regulate osteoporosis by promoting osteoclast differentiation, and miRNA-151a-3p could be a potential therapeutic target for postmenopausal osteoporosis.Keywords: miRNA-151a-3p, postmenopausal osteoporosis, osteoclast differentiation, RAW264.7 cells, THP-1 cellsHe YChen DGuo QShi PYou CFeng YDove Medical Pressarticlemirna-151a-3ppostmenopausal osteoporosisosteoclast differentiationraw264.7 cellsthp-1 cellsGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 16, Pp 1357-1366 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mirna-151a-3p postmenopausal osteoporosis osteoclast differentiation raw264.7 cells thp-1 cells Geriatrics RC952-954.6
spellingShingle	mirna-151a-3p postmenopausal osteoporosis osteoclast differentiation raw264.7 cells thp-1 cells Geriatrics RC952-954.6 He Y Chen D Guo Q Shi P You C Feng Y MicroRNA-151a-3p Functions in the Regulation of Osteoclast Differentiation: Significance to Postmenopausal Osteoporosis
description	Yuehui He, Di Chen, Qian Guo, Pinghua Shi, Conglei You, Yanping Feng Community Medicine Department, Beijing Jishuitan Hospital, Beijing City, 100096, People’s Republic of ChinaCorrespondence: Di ChenCommunity Medicine Department, Beijing Jishuitan Hospital, No. 68, Huinanbei Road, Huilongguan Town, Changping District, Beijing City, 100096, People’s Republic of ChinaEmail zr4560@163.comBackground: Studies have found the pivotal role of miRNAs in the progression of postmenopausal osteoporosis (OP). However, the function of miRNAs in OP is unclear. This study aimed to explore the biological functions of microRNA-151a-3p in OP.Methods: RT-qPCR was employed to assess the expression of microRNA-151a-3p in serum isolated from OP patients and healthy controls. Dual-energy X-ray absorptiometry (DXA) was used to measure the bone mineral density (BMD) of the lumbar spine. The expression levels of c-Fos, NFATc1, and TRAP were tested by Western blot. Ovariectomized (OVX) rats were treated with antago microRNA-151a-3p or antago NC, and then serum and lumbar vertebrae were collected for ELISA and bone histomorphology analysis.Results: The expression of microRNA-151a-3p in postmenopausal women with osteoporosis was significantly up-regulated, and microRNA-151a-3p level was negatively correlated with BMD. During osteoclastogenesis, microRNA-151a-3p level was obviously increased. Overexpression of microRNA-151a-3p promoted the differentiation of RANKL-induced THP-1 and RAW264.7 cells into osteoclasts, whereas silencing of microRNA-151a-3p resulted in the opposite results. Silencing of microRNA-151a-3p in OVX rats altered osteoclastogenesis-related factors and raised BMD.Conclusion: MicroRNA-151a-3p could partly regulate osteoporosis by promoting osteoclast differentiation, and miRNA-151a-3p could be a potential therapeutic target for postmenopausal osteoporosis.Keywords: miRNA-151a-3p, postmenopausal osteoporosis, osteoclast differentiation, RAW264.7 cells, THP-1 cells
format	article
author	He Y Chen D Guo Q Shi P You C Feng Y
author_facet	He Y Chen D Guo Q Shi P You C Feng Y
author_sort	He Y
title	MicroRNA-151a-3p Functions in the Regulation of Osteoclast Differentiation: Significance to Postmenopausal Osteoporosis
title_short	MicroRNA-151a-3p Functions in the Regulation of Osteoclast Differentiation: Significance to Postmenopausal Osteoporosis
title_full	MicroRNA-151a-3p Functions in the Regulation of Osteoclast Differentiation: Significance to Postmenopausal Osteoporosis
title_fullStr	MicroRNA-151a-3p Functions in the Regulation of Osteoclast Differentiation: Significance to Postmenopausal Osteoporosis
title_full_unstemmed	MicroRNA-151a-3p Functions in the Regulation of Osteoclast Differentiation: Significance to Postmenopausal Osteoporosis
title_sort	microrna-151a-3p functions in the regulation of osteoclast differentiation: significance to postmenopausal osteoporosis
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/7c7c87cd3de945ae9fa022ac34cdc227
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MicroRNA-151a-3p Functions in the Regulation of Osteoclast Differentiation: Significance to Postmenopausal Osteoporosis

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