Changes in temperature have opposing effects on current amplitude in α7 and α4β2 nicotinic acetylcholine receptors.

We have examined the effect of temperature on the electrophysiological properties of three neuronal nicotinic acetylcholine receptor (NACHR) subtypes: the rapidly desensitizing homomeric α7 nAChR, the more slowly desensitizing heteromeric α4β2 nAChR and on α7 nAChRs containing a transmembrane mutati...

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Autores principales:	Marie Jindrichova, Stuart J Lansdell, Neil S Millar
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7c849824704a470d9091a92a44e24476
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spelling	oai:doaj.org-article:7c849824704a470d9091a92a44e244762021-11-18T07:27:53ZChanges in temperature have opposing effects on current amplitude in α7 and α4β2 nicotinic acetylcholine receptors.1932-620310.1371/journal.pone.0032073https://doaj.org/article/7c849824704a470d9091a92a44e244762012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22359659/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We have examined the effect of temperature on the electrophysiological properties of three neuronal nicotinic acetylcholine receptor (NACHR) subtypes: the rapidly desensitizing homomeric α7 nAChR, the more slowly desensitizing heteromeric α4β2 nAChR and on α7 nAChRs containing a transmembrane mutation (L247T) that results in dramatically reduced desensitization. In all cases, the functional properties of receptors expressed in Xenopus oocytes at room temperature (RT; 21°C) were compared to those recorded at either physiological temperature (37°C) or at lower temperature (4°C). Alterations in temperature had dramatically differing effects on the amplitude of whole-cell responses detected with these three nAChR subtypes. Compared to responses at RT, the amplitude of agonist-evoked responses with α4β2 nAChRs was increased at high temperature (125±9%, n = 6, P<0.01) and reduced at low temperature (47±5%, n = 6, P<0.01), whereas the amplitude of α7 responses was reduced at high temperature (27±7%, n = 11, P<0.001) and increased at low temperatures (224±16%, n = 10, P<0.001). In contrast to the effects of temperature on α4β2 and wild type α7 nAChRs, the amplitude of α7 nAChRs containing the L247T mutation was unaffected by changes in temperature. In addition, changes in temperature had little or no effect on current amplitude when α7 nAChRs were activated by the largely non-desensitizing allosteric agonist 4BP-TQS. Despite these differing effects of temperature on the amplitude of agonist-evoked responses in different nAChRs, changes in temperature had a consistent effect on the rate of receptor desensitization on all subtypes examined. In all cases, higher temperature resulted in increased rates of desensitization. Thus, it appears that the differing effects of temperature on the amplitudes of whole-cell responses cannot be explained by temperature-induced changes in receptor desensitization rates.Marie JindrichovaStuart J LansdellNeil S MillarPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e32073 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marie Jindrichova Stuart J Lansdell Neil S Millar Changes in temperature have opposing effects on current amplitude in α7 and α4β2 nicotinic acetylcholine receptors.
description	We have examined the effect of temperature on the electrophysiological properties of three neuronal nicotinic acetylcholine receptor (NACHR) subtypes: the rapidly desensitizing homomeric α7 nAChR, the more slowly desensitizing heteromeric α4β2 nAChR and on α7 nAChRs containing a transmembrane mutation (L247T) that results in dramatically reduced desensitization. In all cases, the functional properties of receptors expressed in Xenopus oocytes at room temperature (RT; 21°C) were compared to those recorded at either physiological temperature (37°C) or at lower temperature (4°C). Alterations in temperature had dramatically differing effects on the amplitude of whole-cell responses detected with these three nAChR subtypes. Compared to responses at RT, the amplitude of agonist-evoked responses with α4β2 nAChRs was increased at high temperature (125±9%, n = 6, P<0.01) and reduced at low temperature (47±5%, n = 6, P<0.01), whereas the amplitude of α7 responses was reduced at high temperature (27±7%, n = 11, P<0.001) and increased at low temperatures (224±16%, n = 10, P<0.001). In contrast to the effects of temperature on α4β2 and wild type α7 nAChRs, the amplitude of α7 nAChRs containing the L247T mutation was unaffected by changes in temperature. In addition, changes in temperature had little or no effect on current amplitude when α7 nAChRs were activated by the largely non-desensitizing allosteric agonist 4BP-TQS. Despite these differing effects of temperature on the amplitude of agonist-evoked responses in different nAChRs, changes in temperature had a consistent effect on the rate of receptor desensitization on all subtypes examined. In all cases, higher temperature resulted in increased rates of desensitization. Thus, it appears that the differing effects of temperature on the amplitudes of whole-cell responses cannot be explained by temperature-induced changes in receptor desensitization rates.
format	article
author	Marie Jindrichova Stuart J Lansdell Neil S Millar
author_facet	Marie Jindrichova Stuart J Lansdell Neil S Millar
author_sort	Marie Jindrichova
title	Changes in temperature have opposing effects on current amplitude in α7 and α4β2 nicotinic acetylcholine receptors.
title_short	Changes in temperature have opposing effects on current amplitude in α7 and α4β2 nicotinic acetylcholine receptors.
title_full	Changes in temperature have opposing effects on current amplitude in α7 and α4β2 nicotinic acetylcholine receptors.
title_fullStr	Changes in temperature have opposing effects on current amplitude in α7 and α4β2 nicotinic acetylcholine receptors.
title_full_unstemmed	Changes in temperature have opposing effects on current amplitude in α7 and α4β2 nicotinic acetylcholine receptors.
title_sort	changes in temperature have opposing effects on current amplitude in α7 and α4β2 nicotinic acetylcholine receptors.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/7c849824704a470d9091a92a44e24476
work_keys_str_mv	AT mariejindrichova changesintemperaturehaveopposingeffectsoncurrentamplitudeina7anda4b2nicotinicacetylcholinereceptors AT stuartjlansdell changesintemperaturehaveopposingeffectsoncurrentamplitudeina7anda4b2nicotinicacetylcholinereceptors AT neilsmillar changesintemperaturehaveopposingeffectsoncurrentamplitudeina7anda4b2nicotinicacetylcholinereceptors
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Changes in temperature have opposing effects on current amplitude in α7 and α4β2 nicotinic acetylcholine receptors.

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