Parental LTRs are important in a construct of a stable and efficient replication-competent infectious molecular clone of HIV-1 CRF08_BC.

Circulating recombinant forms (CRFs) of HIV-1 have been identified in southern China in recent years. CRF08_BC is one of the most predominant subtypes circulating in China. In order to study HIV subtype biology and to provide a tool for biotechnological applications, the first full-length replicatio...

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Autores principales:	Qiwei Zhang, Xiaomin Zhang, Hao Wu, Donald Seto, Hao-Jie Zhang, Zhiwei Chen, Chengsong Wan, Bo-Jian Zheng
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7c84b8abfaea41d4b6b06fd3092a09b6
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spelling	oai:doaj.org-article:7c84b8abfaea41d4b6b06fd3092a09b62021-11-18T07:27:38ZParental LTRs are important in a construct of a stable and efficient replication-competent infectious molecular clone of HIV-1 CRF08_BC.1932-620310.1371/journal.pone.0031233https://doaj.org/article/7c84b8abfaea41d4b6b06fd3092a09b62012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22363589/?tool=EBIhttps://doaj.org/toc/1932-6203Circulating recombinant forms (CRFs) of HIV-1 have been identified in southern China in recent years. CRF08_BC is one of the most predominant subtypes circulating in China. In order to study HIV subtype biology and to provide a tool for biotechnological applications, the first full-length replication-competent infectious molecular clone harboring CRF08_BC is reported. The construction of this clone pBRGX indicates that a moderate-copy number vector is required for its amplification in E. coli. In addition, it is shown that the parental CRF08_BC LTRs are important for generating this efficient replication-competent infectious clone. These observations may aid in the construction of infectious clones from other subtypes. Both the pBRGX-derived virus and its parental isolate contain CCR5 tropism. Their full-length genomes were also sequenced, analyzed, compared and deposited in GenBank (JF719819 and JF719818, respectively). The availability of pBRGX as the first replication-competent molecular clone of CRF08_BC provides a useful tool for a wide range of studies of this newly emergent HIV subtype, including the development of HIV vaccine candidates, antiviral drug screening and drug resistance analysis.Qiwei ZhangXiaomin ZhangHao WuDonald SetoHao-Jie ZhangZhiwei ChenChengsong WanBo-Jian ZhengPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e31233 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qiwei Zhang Xiaomin Zhang Hao Wu Donald Seto Hao-Jie Zhang Zhiwei Chen Chengsong Wan Bo-Jian Zheng Parental LTRs are important in a construct of a stable and efficient replication-competent infectious molecular clone of HIV-1 CRF08_BC.
description	Circulating recombinant forms (CRFs) of HIV-1 have been identified in southern China in recent years. CRF08_BC is one of the most predominant subtypes circulating in China. In order to study HIV subtype biology and to provide a tool for biotechnological applications, the first full-length replication-competent infectious molecular clone harboring CRF08_BC is reported. The construction of this clone pBRGX indicates that a moderate-copy number vector is required for its amplification in E. coli. In addition, it is shown that the parental CRF08_BC LTRs are important for generating this efficient replication-competent infectious clone. These observations may aid in the construction of infectious clones from other subtypes. Both the pBRGX-derived virus and its parental isolate contain CCR5 tropism. Their full-length genomes were also sequenced, analyzed, compared and deposited in GenBank (JF719819 and JF719818, respectively). The availability of pBRGX as the first replication-competent molecular clone of CRF08_BC provides a useful tool for a wide range of studies of this newly emergent HIV subtype, including the development of HIV vaccine candidates, antiviral drug screening and drug resistance analysis.
format	article
author	Qiwei Zhang Xiaomin Zhang Hao Wu Donald Seto Hao-Jie Zhang Zhiwei Chen Chengsong Wan Bo-Jian Zheng
author_facet	Qiwei Zhang Xiaomin Zhang Hao Wu Donald Seto Hao-Jie Zhang Zhiwei Chen Chengsong Wan Bo-Jian Zheng
author_sort	Qiwei Zhang
title	Parental LTRs are important in a construct of a stable and efficient replication-competent infectious molecular clone of HIV-1 CRF08_BC.
title_short	Parental LTRs are important in a construct of a stable and efficient replication-competent infectious molecular clone of HIV-1 CRF08_BC.
title_full	Parental LTRs are important in a construct of a stable and efficient replication-competent infectious molecular clone of HIV-1 CRF08_BC.
title_fullStr	Parental LTRs are important in a construct of a stable and efficient replication-competent infectious molecular clone of HIV-1 CRF08_BC.
title_full_unstemmed	Parental LTRs are important in a construct of a stable and efficient replication-competent infectious molecular clone of HIV-1 CRF08_BC.
title_sort	parental ltrs are important in a construct of a stable and efficient replication-competent infectious molecular clone of hiv-1 crf08_bc.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/7c84b8abfaea41d4b6b06fd3092a09b6
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Parental LTRs are important in a construct of a stable and efficient replication-competent infectious molecular clone of HIV-1 CRF08_BC.

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