Lipoic Acid Decreases the Expression of Poly ADP-Ribose Polymerase and Inhibits Apoptosis in Diabetic Rats
Jingfang Chen,1 Qiang Li2 1Department of Endocrinology, Henan Provincial Chest Hospital, Zhengzhou, Henan Province, People’s Republic of China; 2Department of Endocrinology, Shenzhen University General Hospital, Shenzhen, Guangdong Province, People’s Republic of ChinaCorresponden...
Guardado en:
| Autores principales: | , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2020
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/7c92afc3ea3a4b4fa82dfb6d9938bff0 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:7c92afc3ea3a4b4fa82dfb6d9938bff0 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:7c92afc3ea3a4b4fa82dfb6d9938bff02021-12-02T12:06:56ZLipoic Acid Decreases the Expression of Poly ADP-Ribose Polymerase and Inhibits Apoptosis in Diabetic Rats1178-7007https://doaj.org/article/7c92afc3ea3a4b4fa82dfb6d9938bff02020-05-01T00:00:00Zhttps://www.dovepress.com/lipoic-acid-decreases-the-expression-of-poly-adp-ribose-polymerase-and-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Jingfang Chen,1 Qiang Li2 1Department of Endocrinology, Henan Provincial Chest Hospital, Zhengzhou, Henan Province, People’s Republic of China; 2Department of Endocrinology, Shenzhen University General Hospital, Shenzhen, Guangdong Province, People’s Republic of ChinaCorrespondence: Qiang LiDepartment of Endocrinology, Shenzhen University General Hospital, Shenzhen City, Guangdong Province 518055, People’s Republic of ChinaEmail 332706770@qq.comPurpose: To study the effects of lipoic acid on poly ADP-ribose polymerase (PARP) expression and apoptosis in diabetic rats.Materials and Methods: Sprague–Dawley rats (n=30) with high-fat diet- and streptozotocin-induced diabetes were randomly divided into two groups: diabetic model (DM) group and lipoic acid (LA) treatment group; another 10 rats were selected as normal controls (NC). The serum levels of 8-hydroxy-2ʹ-deoxyguanosine, nitrotyrosine, and 8-isoprostane; sciatic nerve cell apoptosis index; and PARP expression were detected in the rats, and morphological changes in the sciatic nerve were recorded.Results: The blood glucose level in the DM and LA groups was significantly higher than that of the NC group (P< 0.01). Compared to the NC group, the DM group showed demyelinating changes to sciatic nerve fibers. PARP expression; serum levels of 8-hydroxy-2-deoxyguanosine, nitrotyrosine, and 8-isoprostane; and the apoptosis index of sciatic nerve cells were significantly higher than those of the NC group (P< 0. 01). Following LA treatment, the above indices showed significant improvement (P< 0.01).Conclusion: Lipoic acid may improve the symptoms of diabetic neuropathy by reducing PARP activity and inhibiting apoptosis.Keywords: lipoic acid, diabetic peripheral neuropathy, poly ADP-ribose polymerase, oxidative stress, apoptosisChen JLi QDove Medical Pressarticlelipoic aciddiabetic peripheral neuropathypoly adp-ribose polymerase，oxidative stressapoptosisSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 1725-1731 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
lipoic acid diabetic peripheral neuropathy poly adp-ribose polymerase，oxidative stress apoptosis Specialties of internal medicine RC581-951 |
| spellingShingle |
lipoic acid diabetic peripheral neuropathy poly adp-ribose polymerase，oxidative stress apoptosis Specialties of internal medicine RC581-951 Chen J Li Q Lipoic Acid Decreases the Expression of Poly ADP-Ribose Polymerase and Inhibits Apoptosis in Diabetic Rats |
| description |
Jingfang Chen,1 Qiang Li2 1Department of Endocrinology, Henan Provincial Chest Hospital, Zhengzhou, Henan Province, People’s Republic of China; 2Department of Endocrinology, Shenzhen University General Hospital, Shenzhen, Guangdong Province, People’s Republic of ChinaCorrespondence: Qiang LiDepartment of Endocrinology, Shenzhen University General Hospital, Shenzhen City, Guangdong Province 518055, People’s Republic of ChinaEmail 332706770@qq.comPurpose: To study the effects of lipoic acid on poly ADP-ribose polymerase (PARP) expression and apoptosis in diabetic rats.Materials and Methods: Sprague–Dawley rats (n=30) with high-fat diet- and streptozotocin-induced diabetes were randomly divided into two groups: diabetic model (DM) group and lipoic acid (LA) treatment group; another 10 rats were selected as normal controls (NC). The serum levels of 8-hydroxy-2ʹ-deoxyguanosine, nitrotyrosine, and 8-isoprostane; sciatic nerve cell apoptosis index; and PARP expression were detected in the rats, and morphological changes in the sciatic nerve were recorded.Results: The blood glucose level in the DM and LA groups was significantly higher than that of the NC group (P< 0.01). Compared to the NC group, the DM group showed demyelinating changes to sciatic nerve fibers. PARP expression; serum levels of 8-hydroxy-2-deoxyguanosine, nitrotyrosine, and 8-isoprostane; and the apoptosis index of sciatic nerve cells were significantly higher than those of the NC group (P< 0. 01). Following LA treatment, the above indices showed significant improvement (P< 0.01).Conclusion: Lipoic acid may improve the symptoms of diabetic neuropathy by reducing PARP activity and inhibiting apoptosis.Keywords: lipoic acid, diabetic peripheral neuropathy, poly ADP-ribose polymerase, oxidative stress, apoptosis |
| format |
article |
| author |
Chen J Li Q |
| author_facet |
Chen J Li Q |
| author_sort |
Chen J |
| title |
Lipoic Acid Decreases the Expression of Poly ADP-Ribose Polymerase and Inhibits Apoptosis in Diabetic Rats |
| title_short |
Lipoic Acid Decreases the Expression of Poly ADP-Ribose Polymerase and Inhibits Apoptosis in Diabetic Rats |
| title_full |
Lipoic Acid Decreases the Expression of Poly ADP-Ribose Polymerase and Inhibits Apoptosis in Diabetic Rats |
| title_fullStr |
Lipoic Acid Decreases the Expression of Poly ADP-Ribose Polymerase and Inhibits Apoptosis in Diabetic Rats |
| title_full_unstemmed |
Lipoic Acid Decreases the Expression of Poly ADP-Ribose Polymerase and Inhibits Apoptosis in Diabetic Rats |
| title_sort |
lipoic acid decreases the expression of poly adp-ribose polymerase and inhibits apoptosis in diabetic rats |
| publisher |
Dove Medical Press |
| publishDate |
2020 |
| url |
https://doaj.org/article/7c92afc3ea3a4b4fa82dfb6d9938bff0 |
| work_keys_str_mv |
AT chenj lipoicaciddecreasestheexpressionofpolyadpribosepolymeraseandinhibitsapoptosisindiabeticrats AT liq lipoicaciddecreasestheexpressionofpolyadpribosepolymeraseandinhibitsapoptosisindiabeticrats |
| _version_ |
1718394698373005312 |