Long-term myofibroblast persistence in the capsular bag contributes to the late spontaneous in-the-bag intraocular lens dislocation

Abstract Late spontaneous in-the-bag intraocular lens (IOL) dislocation is a complication presenting 6 months or later after cataract surgery. We aimed to characterize the cells in the lens capsules (LCs) of 18 patients with spontaneous late in-the-bag IOL dislocation. Patients' average age was...

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Autores principales:	Jovana Bisevac, Natalia S. Anisimova, Richárd Nagymihály, Olav Kristianslund, Kirankumar Katta, Agate Noer, Ilias H. Sharafetdinov, Liv Drolsum, Morten C. Moe, Boris E. Malyugin, Goran Petrovski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7ca01174659e48f28de3394e74533620
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spelling	oai:doaj.org-article:7ca01174659e48f28de3394e745336202021-12-02T12:33:53ZLong-term myofibroblast persistence in the capsular bag contributes to the late spontaneous in-the-bag intraocular lens dislocation10.1038/s41598-020-77207-72045-2322https://doaj.org/article/7ca01174659e48f28de3394e745336202020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77207-7https://doaj.org/toc/2045-2322Abstract Late spontaneous in-the-bag intraocular lens (IOL) dislocation is a complication presenting 6 months or later after cataract surgery. We aimed to characterize the cells in the lens capsules (LCs) of 18 patients with spontaneous late in-the-bag IOL dislocation. Patients' average age was 82.6 ± 1.5 years (range 72–98), and most of them had pseudoexfoliation syndrome (PEX). Cells from the LCs were positive for myofibroblast (αSMA), proliferation (Ki-67, PCNA), early lens development/lens progenitor (SOX2, PAX6), chemokine receptor (CXCR4), and transmembrane (N-cadherin) markers, while negative for epithelial (E-cadherin) marker. Moreover, the cells produced abundant fibronectin, type I and type V collagen in the nearby extracellular matrix (ECM). During ex vivo cultivation of dislocated IOL-LCs in toto, the cells proliferated and likely migrated onto the IOL’s anterior side. EdU proliferation assay confirmed the proliferation potential of the myofibroblasts (MFBs) in dislocated IOL-LCs. Primary cultured lens epithelial cells/MFBs isolated from the LC of dislocated IOLs could induce collagen matrix contraction and continuously proliferated, migrated, and induced ECM remodeling. Taken together, this indicates that long-lived MFBs of dislocated IOLs might contribute to the pathogenic mechanisms in late in-the-bag IOL dislocation.Jovana BisevacNatalia S. AnisimovaRichárd NagymihályOlav KristianslundKirankumar KattaAgate NoerIlias H. SharafetdinovLiv DrolsumMorten C. MoeBoris E. MalyuginGoran PetrovskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-17 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jovana Bisevac Natalia S. Anisimova Richárd Nagymihály Olav Kristianslund Kirankumar Katta Agate Noer Ilias H. Sharafetdinov Liv Drolsum Morten C. Moe Boris E. Malyugin Goran Petrovski Long-term myofibroblast persistence in the capsular bag contributes to the late spontaneous in-the-bag intraocular lens dislocation
description	Abstract Late spontaneous in-the-bag intraocular lens (IOL) dislocation is a complication presenting 6 months or later after cataract surgery. We aimed to characterize the cells in the lens capsules (LCs) of 18 patients with spontaneous late in-the-bag IOL dislocation. Patients' average age was 82.6 ± 1.5 years (range 72–98), and most of them had pseudoexfoliation syndrome (PEX). Cells from the LCs were positive for myofibroblast (αSMA), proliferation (Ki-67, PCNA), early lens development/lens progenitor (SOX2, PAX6), chemokine receptor (CXCR4), and transmembrane (N-cadherin) markers, while negative for epithelial (E-cadherin) marker. Moreover, the cells produced abundant fibronectin, type I and type V collagen in the nearby extracellular matrix (ECM). During ex vivo cultivation of dislocated IOL-LCs in toto, the cells proliferated and likely migrated onto the IOL’s anterior side. EdU proliferation assay confirmed the proliferation potential of the myofibroblasts (MFBs) in dislocated IOL-LCs. Primary cultured lens epithelial cells/MFBs isolated from the LC of dislocated IOLs could induce collagen matrix contraction and continuously proliferated, migrated, and induced ECM remodeling. Taken together, this indicates that long-lived MFBs of dislocated IOLs might contribute to the pathogenic mechanisms in late in-the-bag IOL dislocation.
format	article
author	Jovana Bisevac Natalia S. Anisimova Richárd Nagymihály Olav Kristianslund Kirankumar Katta Agate Noer Ilias H. Sharafetdinov Liv Drolsum Morten C. Moe Boris E. Malyugin Goran Petrovski
author_facet	Jovana Bisevac Natalia S. Anisimova Richárd Nagymihály Olav Kristianslund Kirankumar Katta Agate Noer Ilias H. Sharafetdinov Liv Drolsum Morten C. Moe Boris E. Malyugin Goran Petrovski
author_sort	Jovana Bisevac
title	Long-term myofibroblast persistence in the capsular bag contributes to the late spontaneous in-the-bag intraocular lens dislocation
title_short	Long-term myofibroblast persistence in the capsular bag contributes to the late spontaneous in-the-bag intraocular lens dislocation
title_full	Long-term myofibroblast persistence in the capsular bag contributes to the late spontaneous in-the-bag intraocular lens dislocation
title_fullStr	Long-term myofibroblast persistence in the capsular bag contributes to the late spontaneous in-the-bag intraocular lens dislocation
title_full_unstemmed	Long-term myofibroblast persistence in the capsular bag contributes to the late spontaneous in-the-bag intraocular lens dislocation
title_sort	long-term myofibroblast persistence in the capsular bag contributes to the late spontaneous in-the-bag intraocular lens dislocation
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/7ca01174659e48f28de3394e74533620
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Long-term myofibroblast persistence in the capsular bag contributes to the late spontaneous in-the-bag intraocular lens dislocation

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