Disorder Genes Regulate the Progression of Ischemic Stroke through the NF-κB Signaling Pathway

Stroke is an acute cerebrovascular disease, including ischemic and hemorrhagic stroke. Stroke is the second leading cause of death after ischemic heart disease, which accounts for 9% of the global death toll. To explore the molecular mechanisms of the effects of the dysregulated factors, in the GEO...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wei Wei, Wenqiang Xin, Yufeng Tang, Zhonglun Chen, Yue Heng, Mingjun Pu, Bufan Yang, Jiacai Zuo, Jingfeng Duan
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/7cb43d8c4475474d909485f83353b832
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7cb43d8c4475474d909485f83353b832
record_format	dspace
spelling	oai:doaj.org-article:7cb43d8c4475474d909485f83353b8322021-11-08T02:36:04ZDisorder Genes Regulate the Progression of Ischemic Stroke through the NF-κB Signaling Pathway2314-614110.1155/2021/2464269https://doaj.org/article/7cb43d8c4475474d909485f83353b8322021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/2464269https://doaj.org/toc/2314-6141Stroke is an acute cerebrovascular disease, including ischemic and hemorrhagic stroke. Stroke is the second leading cause of death after ischemic heart disease, which accounts for 9% of the global death toll. To explore the molecular mechanisms of the effects of the dysregulated factors, in the GEO database, we obtained transcriptome data from 24 h/72 h of mice with ischemic stroke and 24 h/72 h of normal mice. We then performed differential gene analysis, coexpression analysis, enrichment analysis, and regulator prediction bioinformatics analysis to identify the potential genes. We made a comparison between the ischemic stroke 72 h and the ischemic stroke for 24 h, and 5103 differential genes were obtained (p<0.05). Four functional barrier modules were obtained by weighted gene coexpression network analysis. The critical genes of each module were ASTL, Zfp472, Fmr1 gene, and Nap1l1. The results of the enrichment analysis showed ncRNA metabolism, microRNAs in cancer, and biosynthesis of amino acids. These three functions and pathways have the most considerable count value. The regulators of the regulatory dysfunction module were predicted by pivotal analysis of TF and noncoding RNA, and critical regulators including NFKB1 (NF-κB1), NFKBIA, CTNNB1, and SP1 were obtained. Finally, the pivotal target gene found that CTNNB1, NFKB1, NFKBia, and Sp1 are involved in 18, 32, 2, and 60 target genes, respectively. Therefore, we believe that NFKB1 and Sp1 have a potential role in the progression of ischemic stroke. The NFKB signaling pathway promotes inflammatory cytokines and regulates the progression of ischemic stroke.Wei WeiWenqiang XinYufeng TangZhonglun ChenYue HengMingjun PuBufan YangJiacai ZuoJingfeng DuanHindawi LimitedarticleMedicineRENBioMed Research International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Wei Wei Wenqiang Xin Yufeng Tang Zhonglun Chen Yue Heng Mingjun Pu Bufan Yang Jiacai Zuo Jingfeng Duan Disorder Genes Regulate the Progression of Ischemic Stroke through the NF-κB Signaling Pathway
description	Stroke is an acute cerebrovascular disease, including ischemic and hemorrhagic stroke. Stroke is the second leading cause of death after ischemic heart disease, which accounts for 9% of the global death toll. To explore the molecular mechanisms of the effects of the dysregulated factors, in the GEO database, we obtained transcriptome data from 24 h/72 h of mice with ischemic stroke and 24 h/72 h of normal mice. We then performed differential gene analysis, coexpression analysis, enrichment analysis, and regulator prediction bioinformatics analysis to identify the potential genes. We made a comparison between the ischemic stroke 72 h and the ischemic stroke for 24 h, and 5103 differential genes were obtained (p<0.05). Four functional barrier modules were obtained by weighted gene coexpression network analysis. The critical genes of each module were ASTL, Zfp472, Fmr1 gene, and Nap1l1. The results of the enrichment analysis showed ncRNA metabolism, microRNAs in cancer, and biosynthesis of amino acids. These three functions and pathways have the most considerable count value. The regulators of the regulatory dysfunction module were predicted by pivotal analysis of TF and noncoding RNA, and critical regulators including NFKB1 (NF-κB1), NFKBIA, CTNNB1, and SP1 were obtained. Finally, the pivotal target gene found that CTNNB1, NFKB1, NFKBia, and Sp1 are involved in 18, 32, 2, and 60 target genes, respectively. Therefore, we believe that NFKB1 and Sp1 have a potential role in the progression of ischemic stroke. The NFKB signaling pathway promotes inflammatory cytokines and regulates the progression of ischemic stroke.
format	article
author	Wei Wei Wenqiang Xin Yufeng Tang Zhonglun Chen Yue Heng Mingjun Pu Bufan Yang Jiacai Zuo Jingfeng Duan
author_facet	Wei Wei Wenqiang Xin Yufeng Tang Zhonglun Chen Yue Heng Mingjun Pu Bufan Yang Jiacai Zuo Jingfeng Duan
author_sort	Wei Wei
title	Disorder Genes Regulate the Progression of Ischemic Stroke through the NF-κB Signaling Pathway
title_short	Disorder Genes Regulate the Progression of Ischemic Stroke through the NF-κB Signaling Pathway
title_full	Disorder Genes Regulate the Progression of Ischemic Stroke through the NF-κB Signaling Pathway
title_fullStr	Disorder Genes Regulate the Progression of Ischemic Stroke through the NF-κB Signaling Pathway
title_full_unstemmed	Disorder Genes Regulate the Progression of Ischemic Stroke through the NF-κB Signaling Pathway
title_sort	disorder genes regulate the progression of ischemic stroke through the nf-κb signaling pathway
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/7cb43d8c4475474d909485f83353b832
work_keys_str_mv	AT weiwei disordergenesregulatetheprogressionofischemicstrokethroughthenfkbsignalingpathway AT wenqiangxin disordergenesregulatetheprogressionofischemicstrokethroughthenfkbsignalingpathway AT yufengtang disordergenesregulatetheprogressionofischemicstrokethroughthenfkbsignalingpathway AT zhonglunchen disordergenesregulatetheprogressionofischemicstrokethroughthenfkbsignalingpathway AT yueheng disordergenesregulatetheprogressionofischemicstrokethroughthenfkbsignalingpathway AT mingjunpu disordergenesregulatetheprogressionofischemicstrokethroughthenfkbsignalingpathway AT bufanyang disordergenesregulatetheprogressionofischemicstrokethroughthenfkbsignalingpathway AT jiacaizuo disordergenesregulatetheprogressionofischemicstrokethroughthenfkbsignalingpathway AT jingfengduan disordergenesregulatetheprogressionofischemicstrokethroughthenfkbsignalingpathway
_version_	1718443199247155200

Disorder Genes Regulate the Progression of Ischemic Stroke through the NF-κB Signaling Pathway

Ejemplares similares