Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide

Chemically modifying proteins is hard to achieve selectively without purifying the target protein. Here, the authors present a method to modify proteins on lysine residues in living cells quicker than via known approaches and show that it can be used to develop protein covalent inhibitors.

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Autores principales:	Tomonori Tamura, Tsuyoshi Ueda, Taiki Goto, Taku Tsukidate, Yonatan Shapira, Yuki Nishikawa, Alma Fujisawa, Itaru Hamachi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/7cb4a42cb77f46bb9985a6f970724a6a
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spelling	oai:doaj.org-article:7cb4a42cb77f46bb9985a6f970724a6a2021-12-02T16:49:19ZRapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide10.1038/s41467-018-04343-02041-1723https://doaj.org/article/7cb4a42cb77f46bb9985a6f970724a6a2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-04343-0https://doaj.org/toc/2041-1723Chemically modifying proteins is hard to achieve selectively without purifying the target protein. Here, the authors present a method to modify proteins on lysine residues in living cells quicker than via known approaches and show that it can be used to develop protein covalent inhibitors.Tomonori TamuraTsuyoshi UedaTaiki GotoTaku TsukidateYonatan ShapiraYuki NishikawaAlma FujisawaItaru HamachiNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Tomonori Tamura Tsuyoshi Ueda Taiki Goto Taku Tsukidate Yonatan Shapira Yuki Nishikawa Alma Fujisawa Itaru Hamachi Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide
description	Chemically modifying proteins is hard to achieve selectively without purifying the target protein. Here, the authors present a method to modify proteins on lysine residues in living cells quicker than via known approaches and show that it can be used to develop protein covalent inhibitors.
format	article
author	Tomonori Tamura Tsuyoshi Ueda Taiki Goto Taku Tsukidate Yonatan Shapira Yuki Nishikawa Alma Fujisawa Itaru Hamachi
author_facet	Tomonori Tamura Tsuyoshi Ueda Taiki Goto Taku Tsukidate Yonatan Shapira Yuki Nishikawa Alma Fujisawa Itaru Hamachi
author_sort	Tomonori Tamura
title	Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide
title_short	Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide
title_full	Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide
title_fullStr	Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide
title_full_unstemmed	Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide
title_sort	rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed n-acyl-n-alkyl sulfonamide
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/7cb4a42cb77f46bb9985a6f970724a6a
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Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide

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