Transient Triplet Differential (TTD) Method for Background Free Photoacoustic Imaging

Abstract With the capability of presenting endogenous tissue contrast or exogenous contrast agents in deep biological samples at high spatial resolution, photoacoustic (PA) imaging has shown significant potential for many preclinical and clinical applications. However, due to strong background signa...

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Autores principales:	Joel W. Y. Tan, Chang H. Lee, Raoul Kopelman, Xueding Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7cb76454b1d0443789671d781c541b2d
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spelling	oai:doaj.org-article:7cb76454b1d0443789671d781c541b2d2021-12-02T15:07:58ZTransient Triplet Differential (TTD) Method for Background Free Photoacoustic Imaging10.1038/s41598-018-27578-92045-2322https://doaj.org/article/7cb76454b1d0443789671d781c541b2d2018-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-27578-9https://doaj.org/toc/2045-2322Abstract With the capability of presenting endogenous tissue contrast or exogenous contrast agents in deep biological samples at high spatial resolution, photoacoustic (PA) imaging has shown significant potential for many preclinical and clinical applications. However, due to strong background signals from various intrinsic chromophores in biological tissue, such as hemoglobin, achieving highly sensitive PA imaging of targeting probes labeled by contrast agents has remained a challenge. In this study, we introduce a novel technique called transient triplet differential (TTD) imaging which allows for substantial reduction of tissue background signals. TTD imaging detects directly the triplet state absorption, which is a special characteristic of phosphorescence capable dyes not normally present among intrinsic chromophores of biological tissue. Thus, these triplet state absorption PA images can facilitate “true” background free molecular imaging. We prepared a known phosphorescent dye probe, methylene blue conjugated polyacrylamide nanoparticles, with peak absorption at 660 nm and peak lowest triplet state absorption at 840 nm. We find, through studies on phantoms and on an in vivo tumor model, that TTD imaging can generate a superior contrast-to-noise ratio, compared to other image enhancement techniques, through the removal of noise generated by strongly absorbing intrinsic chromophores, regardless of their identity.Joel W. Y. TanChang H. LeeRaoul KopelmanXueding WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joel W. Y. Tan Chang H. Lee Raoul Kopelman Xueding Wang Transient Triplet Differential (TTD) Method for Background Free Photoacoustic Imaging
description	Abstract With the capability of presenting endogenous tissue contrast or exogenous contrast agents in deep biological samples at high spatial resolution, photoacoustic (PA) imaging has shown significant potential for many preclinical and clinical applications. However, due to strong background signals from various intrinsic chromophores in biological tissue, such as hemoglobin, achieving highly sensitive PA imaging of targeting probes labeled by contrast agents has remained a challenge. In this study, we introduce a novel technique called transient triplet differential (TTD) imaging which allows for substantial reduction of tissue background signals. TTD imaging detects directly the triplet state absorption, which is a special characteristic of phosphorescence capable dyes not normally present among intrinsic chromophores of biological tissue. Thus, these triplet state absorption PA images can facilitate “true” background free molecular imaging. We prepared a known phosphorescent dye probe, methylene blue conjugated polyacrylamide nanoparticles, with peak absorption at 660 nm and peak lowest triplet state absorption at 840 nm. We find, through studies on phantoms and on an in vivo tumor model, that TTD imaging can generate a superior contrast-to-noise ratio, compared to other image enhancement techniques, through the removal of noise generated by strongly absorbing intrinsic chromophores, regardless of their identity.
format	article
author	Joel W. Y. Tan Chang H. Lee Raoul Kopelman Xueding Wang
author_facet	Joel W. Y. Tan Chang H. Lee Raoul Kopelman Xueding Wang
author_sort	Joel W. Y. Tan
title	Transient Triplet Differential (TTD) Method for Background Free Photoacoustic Imaging
title_short	Transient Triplet Differential (TTD) Method for Background Free Photoacoustic Imaging
title_full	Transient Triplet Differential (TTD) Method for Background Free Photoacoustic Imaging
title_fullStr	Transient Triplet Differential (TTD) Method for Background Free Photoacoustic Imaging
title_full_unstemmed	Transient Triplet Differential (TTD) Method for Background Free Photoacoustic Imaging
title_sort	transient triplet differential (ttd) method for background free photoacoustic imaging
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/7cb76454b1d0443789671d781c541b2d
work_keys_str_mv	AT joelwytan transienttripletdifferentialttdmethodforbackgroundfreephotoacousticimaging AT changhlee transienttripletdifferentialttdmethodforbackgroundfreephotoacousticimaging AT raoulkopelman transienttripletdifferentialttdmethodforbackgroundfreephotoacousticimaging AT xuedingwang transienttripletdifferentialttdmethodforbackgroundfreephotoacousticimaging
_version_	1718388324943527936

Transient Triplet Differential (TTD) Method for Background Free Photoacoustic Imaging

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