Discrepancies in Serology-Based and Nucleic Acid-Based Detection and Quantitation of Tomato Spotted Wilt Orthotospovirus in Leaf and Root Tissues from Symptomatic and Asymptomatic Peanut Plants

Thrips-transmitted tomato spotted wilt orthotospovirus (TSWV) causes spotted wilt disease in peanuts. A serological test (DAS-ELISA) is often used to detect TSWV in peanut leaf samples. However, in a few studies, DAS-ELISA detected more TSWV infection in root than leaf samples. It was not clear if t...

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Autores principales:	Pin-Chu Lai, Mark R. Abney, Yi-Ju Chen, Sudeep Bag, Rajagopalbabu Srinivasan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Arachis hypogaea</i> L. spotted wilt serological detection overestimation tissue type virus accumulation Medicine R
Acceso en línea:	https://doaj.org/article/7cc290021229493782166f4c5e1ed74a
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spelling	oai:doaj.org-article:7cc290021229493782166f4c5e1ed74a2021-11-25T18:38:32ZDiscrepancies in Serology-Based and Nucleic Acid-Based Detection and Quantitation of Tomato Spotted Wilt Orthotospovirus in Leaf and Root Tissues from Symptomatic and Asymptomatic Peanut Plants10.3390/pathogens101114762076-0817https://doaj.org/article/7cc290021229493782166f4c5e1ed74a2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1476https://doaj.org/toc/2076-0817Thrips-transmitted tomato spotted wilt orthotospovirus (TSWV) causes spotted wilt disease in peanuts. A serological test (DAS-ELISA) is often used to detect TSWV in peanut leaf samples. However, in a few studies, DAS-ELISA detected more TSWV infection in root than leaf samples. It was not clear if the increased detection was due to increased TSWV accumulation in root tissue or merely an overestimation. Additionally, it was unclear if TSWV detection in asymptomatic plants would be affected by the detection technique. TSWV infection in leaf and root tissue from symptomatic and asymptomatic plants was compared via DAS-ELISA, RT-PCR, and RT-qPCR. TSWV incidence did not vary by DAS-ELISA, RT-PCR, and RT-qPCR in leaf and root samples of symptomatic plants or in leaf samples of asymptomatic plants. In contrast, significantly more TSWV infection and virus load were detected in root samples of asymptomatic plants via DAS-ELISA than other techniques suggesting that DAS-ELISA overestimated TSWV incidence and load. TSWV loads from symptomatic plants via RT-qPCR were higher in leaf than root samples, while TSWV loads in leaf and root samples from asymptomatic plants were not different but were lower than those in symptomatic plants. These findings suggested that peanut tissue type and detection technique could affect accurate TSWV detection and/or quantitation.Pin-Chu LaiMark R. AbneyYi-Ju ChenSudeep BagRajagopalbabu SrinivasanMDPI AGarticle<i>Arachis hypogaea</i> L.spotted wiltserological detectionoverestimationtissue typevirus accumulationMedicineRENPathogens, Vol 10, Iss 1476, p 1476 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Arachis hypogaea</i> L. spotted wilt serological detection overestimation tissue type virus accumulation Medicine R
spellingShingle	<i>Arachis hypogaea</i> L. spotted wilt serological detection overestimation tissue type virus accumulation Medicine R Pin-Chu Lai Mark R. Abney Yi-Ju Chen Sudeep Bag Rajagopalbabu Srinivasan Discrepancies in Serology-Based and Nucleic Acid-Based Detection and Quantitation of Tomato Spotted Wilt Orthotospovirus in Leaf and Root Tissues from Symptomatic and Asymptomatic Peanut Plants
description	Thrips-transmitted tomato spotted wilt orthotospovirus (TSWV) causes spotted wilt disease in peanuts. A serological test (DAS-ELISA) is often used to detect TSWV in peanut leaf samples. However, in a few studies, DAS-ELISA detected more TSWV infection in root than leaf samples. It was not clear if the increased detection was due to increased TSWV accumulation in root tissue or merely an overestimation. Additionally, it was unclear if TSWV detection in asymptomatic plants would be affected by the detection technique. TSWV infection in leaf and root tissue from symptomatic and asymptomatic plants was compared via DAS-ELISA, RT-PCR, and RT-qPCR. TSWV incidence did not vary by DAS-ELISA, RT-PCR, and RT-qPCR in leaf and root samples of symptomatic plants or in leaf samples of asymptomatic plants. In contrast, significantly more TSWV infection and virus load were detected in root samples of asymptomatic plants via DAS-ELISA than other techniques suggesting that DAS-ELISA overestimated TSWV incidence and load. TSWV loads from symptomatic plants via RT-qPCR were higher in leaf than root samples, while TSWV loads in leaf and root samples from asymptomatic plants were not different but were lower than those in symptomatic plants. These findings suggested that peanut tissue type and detection technique could affect accurate TSWV detection and/or quantitation.
format	article
author	Pin-Chu Lai Mark R. Abney Yi-Ju Chen Sudeep Bag Rajagopalbabu Srinivasan
author_facet	Pin-Chu Lai Mark R. Abney Yi-Ju Chen Sudeep Bag Rajagopalbabu Srinivasan
author_sort	Pin-Chu Lai
title	Discrepancies in Serology-Based and Nucleic Acid-Based Detection and Quantitation of Tomato Spotted Wilt Orthotospovirus in Leaf and Root Tissues from Symptomatic and Asymptomatic Peanut Plants
title_short	Discrepancies in Serology-Based and Nucleic Acid-Based Detection and Quantitation of Tomato Spotted Wilt Orthotospovirus in Leaf and Root Tissues from Symptomatic and Asymptomatic Peanut Plants
title_full	Discrepancies in Serology-Based and Nucleic Acid-Based Detection and Quantitation of Tomato Spotted Wilt Orthotospovirus in Leaf and Root Tissues from Symptomatic and Asymptomatic Peanut Plants
title_fullStr	Discrepancies in Serology-Based and Nucleic Acid-Based Detection and Quantitation of Tomato Spotted Wilt Orthotospovirus in Leaf and Root Tissues from Symptomatic and Asymptomatic Peanut Plants
title_full_unstemmed	Discrepancies in Serology-Based and Nucleic Acid-Based Detection and Quantitation of Tomato Spotted Wilt Orthotospovirus in Leaf and Root Tissues from Symptomatic and Asymptomatic Peanut Plants
title_sort	discrepancies in serology-based and nucleic acid-based detection and quantitation of tomato spotted wilt orthotospovirus in leaf and root tissues from symptomatic and asymptomatic peanut plants
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7cc290021229493782166f4c5e1ed74a
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Discrepancies in Serology-Based and Nucleic Acid-Based Detection and Quantitation of Tomato Spotted Wilt Orthotospovirus in Leaf and Root Tissues from Symptomatic and Asymptomatic Peanut Plants

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