Mitochondrial Imbalance as a New Approach to the Study of Fibromyalgia

Antonio Martínez-Lara,1 Ana María Moreno-Fernández,2 Maripaz Jiménez-Guerrero,1 Claudia Díaz-López,1 Manuel De-Miguel,2 David Cotán,1,3 José Antonio Sánchez-Alcázar3 1Pronacera Therapeutics...

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Autores principales:	Martínez-Lara A, Moreno-Fernández AM, Jiménez-Guerrero M, Díaz-López C, De-Miguel M, Cotán D, Sánchez-Alcázar JA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	fibromyalgia diagnosis mitochondria chronic pain. Diseases of the musculoskeletal system RC925-935
Acceso en línea:	https://doaj.org/article/7cc2bd216bfc4c9a97c04e55bf15a11c
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spelling	oai:doaj.org-article:7cc2bd216bfc4c9a97c04e55bf15a11c2021-12-02T13:07:23ZMitochondrial Imbalance as a New Approach to the Study of Fibromyalgia1179-156Xhttps://doaj.org/article/7cc2bd216bfc4c9a97c04e55bf15a11c2020-08-01T00:00:00Zhttps://www.dovepress.com/mitochondrial-imbalance-as-a-new-approach-to-the-study-of-fibromyalgia-peer-reviewed-article-OARRRhttps://doaj.org/toc/1179-156XAntonio Martínez-Lara,1 Ana María Moreno-Fernández,2 Maripaz Jiménez-Guerrero,1 Claudia Díaz-López,1 Manuel De-Miguel,2 David Cotán,1,3 José Antonio Sánchez-Alcázar3 1Pronacera Therapeutics S.L., Seville, Spain; 2Departamento de Citología e Histología Normal y Patológica, Facultad de Medicina, Universidad de Sevilla, Seville, Spain; 3Centro Andaluz de Biología del Desarrollo (CABD-CSIC-Universidad Pablo de Olavide), Seville 41013, SpainCorrespondence: David Cotán Tel +34 615 41 26 42Email david.cotan@hotmail.comBackground: Fibromyalgia (FM) is a common chronic pain disease, whose pathogenic mechanism still remains elusive. Oxidative stress markers and impaired bioenergetics homeostasis have been proposed as relevant events in the pathogenesis of the disease. Hence, the aim of the study is to analyse the potential biomarkers of mitochondrial imbalance in FM patients along with coenzyme Q10 (CoQ10) as a possible treatment.Methods: The symptomatology of patients was recorded with an adaption of the Fibromyalgia Impact Questionnaire (FIQ). Mitochondrial imbalance was tested from blood extraction and serum isolation in 33 patients diagnosed with FM and 30 healthy controls. Western blot and HPLC techniques were performed to study the different parameters. Finally, bioinformatic analysis of machine learning was performed to predict possible associations of results.Results: CoQ10 parameter did not show evidence to be a good marker of the disease, as the values are not significantly different between control and patient groups (Student’s t-test, CI 95%). For this reason, the focus of the study changed into the ratio between mitochondrial mass and autophagy levels. The bioinformatics analysis showed a possible association between this ratio and patients’ symptomatology. Finally, the effects of coenzyme Q10 as a potential treatment for the disease were different within patients, and its efficacy may be related to the initial mitochondrial status. However, there is no statistical significance due to limitations within the sample size.Conclusion: Our study supports the hypothesis that an imbalance in mitochondrial homeostasis is involved in the FM pathogenesis. However, whether the increase in oxidative stress is the result of mitochondrial imbalance or the cause of this disease remains an open question. The measurement of this imbalance might be used as a preliminary biomarker for the diagnosis and follow-up of patients with FM, and even for the evaluation of the effects of the different antioxidants therapies.Keywords: fibromyalgia, diagnosis, mitochondria, chronic painMartínez-Lara AMoreno-Fernández AMJiménez-Guerrero MDíaz-López CDe-Miguel MCotán DSánchez-Alcázar JADove Medical Pressarticlefibromyalgiadiagnosismitochondriachronic pain.Diseases of the musculoskeletal systemRC925-935ENOpen Access Rheumatology: Research and Reviews, Vol Volume 12, Pp 175-185 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	fibromyalgia diagnosis mitochondria chronic pain. Diseases of the musculoskeletal system RC925-935
spellingShingle	fibromyalgia diagnosis mitochondria chronic pain. Diseases of the musculoskeletal system RC925-935 Martínez-Lara A Moreno-Fernández AM Jiménez-Guerrero M Díaz-López C De-Miguel M Cotán D Sánchez-Alcázar JA Mitochondrial Imbalance as a New Approach to the Study of Fibromyalgia
description	Antonio Martínez-Lara,1 Ana María Moreno-Fernández,2 Maripaz Jiménez-Guerrero,1 Claudia Díaz-López,1 Manuel De-Miguel,2 David Cotán,1,3 José Antonio Sánchez-Alcázar3 1Pronacera Therapeutics S.L., Seville, Spain; 2Departamento de Citología e Histología Normal y Patológica, Facultad de Medicina, Universidad de Sevilla, Seville, Spain; 3Centro Andaluz de Biología del Desarrollo (CABD-CSIC-Universidad Pablo de Olavide), Seville 41013, SpainCorrespondence: David Cotán Tel +34 615 41 26 42Email david.cotan@hotmail.comBackground: Fibromyalgia (FM) is a common chronic pain disease, whose pathogenic mechanism still remains elusive. Oxidative stress markers and impaired bioenergetics homeostasis have been proposed as relevant events in the pathogenesis of the disease. Hence, the aim of the study is to analyse the potential biomarkers of mitochondrial imbalance in FM patients along with coenzyme Q10 (CoQ10) as a possible treatment.Methods: The symptomatology of patients was recorded with an adaption of the Fibromyalgia Impact Questionnaire (FIQ). Mitochondrial imbalance was tested from blood extraction and serum isolation in 33 patients diagnosed with FM and 30 healthy controls. Western blot and HPLC techniques were performed to study the different parameters. Finally, bioinformatic analysis of machine learning was performed to predict possible associations of results.Results: CoQ10 parameter did not show evidence to be a good marker of the disease, as the values are not significantly different between control and patient groups (Student’s t-test, CI 95%). For this reason, the focus of the study changed into the ratio between mitochondrial mass and autophagy levels. The bioinformatics analysis showed a possible association between this ratio and patients’ symptomatology. Finally, the effects of coenzyme Q10 as a potential treatment for the disease were different within patients, and its efficacy may be related to the initial mitochondrial status. However, there is no statistical significance due to limitations within the sample size.Conclusion: Our study supports the hypothesis that an imbalance in mitochondrial homeostasis is involved in the FM pathogenesis. However, whether the increase in oxidative stress is the result of mitochondrial imbalance or the cause of this disease remains an open question. The measurement of this imbalance might be used as a preliminary biomarker for the diagnosis and follow-up of patients with FM, and even for the evaluation of the effects of the different antioxidants therapies.Keywords: fibromyalgia, diagnosis, mitochondria, chronic pain
format	article
author	Martínez-Lara A Moreno-Fernández AM Jiménez-Guerrero M Díaz-López C De-Miguel M Cotán D Sánchez-Alcázar JA
author_facet	Martínez-Lara A Moreno-Fernández AM Jiménez-Guerrero M Díaz-López C De-Miguel M Cotán D Sánchez-Alcázar JA
author_sort	Martínez-Lara A
title	Mitochondrial Imbalance as a New Approach to the Study of Fibromyalgia
title_short	Mitochondrial Imbalance as a New Approach to the Study of Fibromyalgia
title_full	Mitochondrial Imbalance as a New Approach to the Study of Fibromyalgia
title_fullStr	Mitochondrial Imbalance as a New Approach to the Study of Fibromyalgia
title_full_unstemmed	Mitochondrial Imbalance as a New Approach to the Study of Fibromyalgia
title_sort	mitochondrial imbalance as a new approach to the study of fibromyalgia
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/7cc2bd216bfc4c9a97c04e55bf15a11c
work_keys_str_mv	AT martinezlaraa mitochondrialimbalanceasanewapproachtothestudyoffibromyalgia AT morenofernandezam mitochondrialimbalanceasanewapproachtothestudyoffibromyalgia AT jimenezguerrerom mitochondrialimbalanceasanewapproachtothestudyoffibromyalgia AT diazlopezc mitochondrialimbalanceasanewapproachtothestudyoffibromyalgia AT demiguelm mitochondrialimbalanceasanewapproachtothestudyoffibromyalgia AT cotand mitochondrialimbalanceasanewapproachtothestudyoffibromyalgia AT sanchezalcazarja mitochondrialimbalanceasanewapproachtothestudyoffibromyalgia
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Mitochondrial Imbalance as a New Approach to the Study of Fibromyalgia

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