Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems

Super-resolution microscopy often suffers from low contrast and slow recording times. Here the authors present an optical implementation which makes the fluorescent proteins’ ON–OFF switching cycles more efficient, enhancing contrast and spatio-temporal resolution in 3D cell and tissue imaging.

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Detalles Bibliográficos
Autores principales: Luciano A. Masullo, Andreas Bodén, Francesca Pennacchietti, Giovanna Coceano, Michael Ratz, Ilaria Testa
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/7cc42c8ba8c447d2a6cf7482a6916e8d
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Descripción
Sumario:Super-resolution microscopy often suffers from low contrast and slow recording times. Here the authors present an optical implementation which makes the fluorescent proteins’ ON–OFF switching cycles more efficient, enhancing contrast and spatio-temporal resolution in 3D cell and tissue imaging.