Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems

Super-resolution microscopy often suffers from low contrast and slow recording times. Here the authors present an optical implementation which makes the fluorescent proteins’ ON–OFF switching cycles more efficient, enhancing contrast and spatio-temporal resolution in 3D cell and tissue imaging.

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Auteurs principaux:	Luciano A. Masullo, Andreas Bodén, Francesca Pennacchietti, Giovanna Coceano, Michael Ratz, Ilaria Testa
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2018
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/7cc42c8ba8c447d2a6cf7482a6916e8d
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id	oai:doaj.org-article:7cc42c8ba8c447d2a6cf7482a6916e8d
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spelling	oai:doaj.org-article:7cc42c8ba8c447d2a6cf7482a6916e8d2021-12-02T17:33:02ZEnhanced photon collection enables four dimensional fluorescence nanoscopy of living systems10.1038/s41467-018-05799-w2041-1723https://doaj.org/article/7cc42c8ba8c447d2a6cf7482a6916e8d2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05799-whttps://doaj.org/toc/2041-1723Super-resolution microscopy often suffers from low contrast and slow recording times. Here the authors present an optical implementation which makes the fluorescent proteins’ ON–OFF switching cycles more efficient, enhancing contrast and spatio-temporal resolution in 3D cell and tissue imaging.Luciano A. MasulloAndreas BodénFrancesca PennacchiettiGiovanna CoceanoMichael RatzIlaria TestaNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Luciano A. Masullo Andreas Bodén Francesca Pennacchietti Giovanna Coceano Michael Ratz Ilaria Testa Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems
description	Super-resolution microscopy often suffers from low contrast and slow recording times. Here the authors present an optical implementation which makes the fluorescent proteins’ ON–OFF switching cycles more efficient, enhancing contrast and spatio-temporal resolution in 3D cell and tissue imaging.
format	article
author	Luciano A. Masullo Andreas Bodén Francesca Pennacchietti Giovanna Coceano Michael Ratz Ilaria Testa
author_facet	Luciano A. Masullo Andreas Bodén Francesca Pennacchietti Giovanna Coceano Michael Ratz Ilaria Testa
author_sort	Luciano A. Masullo
title	Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems
title_short	Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems
title_full	Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems
title_fullStr	Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems
title_full_unstemmed	Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems
title_sort	enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/7cc42c8ba8c447d2a6cf7482a6916e8d
work_keys_str_mv	AT lucianoamasullo enhancedphotoncollectionenablesfourdimensionalfluorescencenanoscopyoflivingsystems AT andreasboden enhancedphotoncollectionenablesfourdimensionalfluorescencenanoscopyoflivingsystems AT francescapennacchietti enhancedphotoncollectionenablesfourdimensionalfluorescencenanoscopyoflivingsystems AT giovannacoceano enhancedphotoncollectionenablesfourdimensionalfluorescencenanoscopyoflivingsystems AT michaelratz enhancedphotoncollectionenablesfourdimensionalfluorescencenanoscopyoflivingsystems AT ilariatesta enhancedphotoncollectionenablesfourdimensionalfluorescencenanoscopyoflivingsystems
_version_	1718380069625266176

Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems

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