Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems

Super-resolution microscopy often suffers from low contrast and slow recording times. Here the authors present an optical implementation which makes the fluorescent proteins’ ON–OFF switching cycles more efficient, enhancing contrast and spatio-temporal resolution in 3D cell and tissue imaging.

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Bibliographic Details
Main Authors: Luciano A. Masullo, Andreas Bodén, Francesca Pennacchietti, Giovanna Coceano, Michael Ratz, Ilaria Testa
Format: article
Language:EN
Published: Nature Portfolio 2018
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Q
Online Access:https://doaj.org/article/7cc42c8ba8c447d2a6cf7482a6916e8d
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