Single dose of multi-clade virus-like particle vaccine protects chickens against clade 2.3.2.1 and clade 2.3.4.4 highly pathogenic avian influenza viruses

Abstract Virus-like particles (VLPs) are recognized as an alternative vaccine platform that provide effective protection against various highly pathogenic avian influenza viruses (HPAIVs). Here, we developed multi-clade VLPs expressing two HAs (a chimera of clade 2.3.2.1c and clade 2.3.4.4c HA) with...

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Autores principales: Yong-Myung Kang, Hyun-Kyu Cho, Ju Hun Kim, Su Jin Lee, Seo-Jeong Park, Do-Young Kim, Seong Yup Kim, Jung-won Park, Myoung-Heon Lee, Min-Chul Kim, Hyun-Mi Kang
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/7cc92c40f6c94ae3abae698aad48eac6
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spelling oai:doaj.org-article:7cc92c40f6c94ae3abae698aad48eac62021-12-02T16:10:36ZSingle dose of multi-clade virus-like particle vaccine protects chickens against clade 2.3.2.1 and clade 2.3.4.4 highly pathogenic avian influenza viruses10.1038/s41598-021-93060-82045-2322https://doaj.org/article/7cc92c40f6c94ae3abae698aad48eac62021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93060-8https://doaj.org/toc/2045-2322Abstract Virus-like particles (VLPs) are recognized as an alternative vaccine platform that provide effective protection against various highly pathogenic avian influenza viruses (HPAIVs). Here, we developed multi-clade VLPs expressing two HAs (a chimera of clade 2.3.2.1c and clade 2.3.4.4c HA) within a single vector. We then compared its protective efficacy with that of a monovalent VLP and evaluated its potency against each homologous strain. Chickens vaccinated with the multi-clade VLP shed less virus and were better protected against challenge than birds receiving monovalent vaccines. Single vaccination with a multi-clade VLP resulted in 100% survival, with no clinical symptoms and high levels of pre-challenge protective immunity (7.6–8.5 log2). Moreover, the multi-clade VLP showed high productivity (128–256 HAU) both in the laboratory and on a large scale, making it cheaper than whole inactivated vaccines produced in eggs. However, the PD50 (protective dose 50%) of the multi-clade VLP against clades 2.3.2.1c and 2.3.4.4c was < 50 PD50 (28 and 42 PD50, respectively), and effective antibody response was maintained for 2–3 months. This multi-clade VLP protects against both clades of HPAI viruses and can be produced in high amounts at low cost. Thus, the vaccine has potential as a pandemic preparedness vaccine.Yong-Myung KangHyun-Kyu ChoJu Hun KimSu Jin LeeSeo-Jeong ParkDo-Young KimSeong Yup KimJung-won ParkMyoung-Heon LeeMin-Chul KimHyun-Mi KangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Yong-Myung Kang
Hyun-Kyu Cho
Ju Hun Kim
Su Jin Lee
Seo-Jeong Park
Do-Young Kim
Seong Yup Kim
Jung-won Park
Myoung-Heon Lee
Min-Chul Kim
Hyun-Mi Kang
Single dose of multi-clade virus-like particle vaccine protects chickens against clade 2.3.2.1 and clade 2.3.4.4 highly pathogenic avian influenza viruses
description Abstract Virus-like particles (VLPs) are recognized as an alternative vaccine platform that provide effective protection against various highly pathogenic avian influenza viruses (HPAIVs). Here, we developed multi-clade VLPs expressing two HAs (a chimera of clade 2.3.2.1c and clade 2.3.4.4c HA) within a single vector. We then compared its protective efficacy with that of a monovalent VLP and evaluated its potency against each homologous strain. Chickens vaccinated with the multi-clade VLP shed less virus and were better protected against challenge than birds receiving monovalent vaccines. Single vaccination with a multi-clade VLP resulted in 100% survival, with no clinical symptoms and high levels of pre-challenge protective immunity (7.6–8.5 log2). Moreover, the multi-clade VLP showed high productivity (128–256 HAU) both in the laboratory and on a large scale, making it cheaper than whole inactivated vaccines produced in eggs. However, the PD50 (protective dose 50%) of the multi-clade VLP against clades 2.3.2.1c and 2.3.4.4c was < 50 PD50 (28 and 42 PD50, respectively), and effective antibody response was maintained for 2–3 months. This multi-clade VLP protects against both clades of HPAI viruses and can be produced in high amounts at low cost. Thus, the vaccine has potential as a pandemic preparedness vaccine.
format article
author Yong-Myung Kang
Hyun-Kyu Cho
Ju Hun Kim
Su Jin Lee
Seo-Jeong Park
Do-Young Kim
Seong Yup Kim
Jung-won Park
Myoung-Heon Lee
Min-Chul Kim
Hyun-Mi Kang
author_facet Yong-Myung Kang
Hyun-Kyu Cho
Ju Hun Kim
Su Jin Lee
Seo-Jeong Park
Do-Young Kim
Seong Yup Kim
Jung-won Park
Myoung-Heon Lee
Min-Chul Kim
Hyun-Mi Kang
author_sort Yong-Myung Kang
title Single dose of multi-clade virus-like particle vaccine protects chickens against clade 2.3.2.1 and clade 2.3.4.4 highly pathogenic avian influenza viruses
title_short Single dose of multi-clade virus-like particle vaccine protects chickens against clade 2.3.2.1 and clade 2.3.4.4 highly pathogenic avian influenza viruses
title_full Single dose of multi-clade virus-like particle vaccine protects chickens against clade 2.3.2.1 and clade 2.3.4.4 highly pathogenic avian influenza viruses
title_fullStr Single dose of multi-clade virus-like particle vaccine protects chickens against clade 2.3.2.1 and clade 2.3.4.4 highly pathogenic avian influenza viruses
title_full_unstemmed Single dose of multi-clade virus-like particle vaccine protects chickens against clade 2.3.2.1 and clade 2.3.4.4 highly pathogenic avian influenza viruses
title_sort single dose of multi-clade virus-like particle vaccine protects chickens against clade 2.3.2.1 and clade 2.3.4.4 highly pathogenic avian influenza viruses
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/7cc92c40f6c94ae3abae698aad48eac6
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