Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.).

In mammals, several genes involved in liver lipid and cholesterol homeostasis are rhythmically expressed with expression shown to be regulated by clock genes via Rev-erb 1α. In order to elucidate clock gene regulation of genes involved in lipid metabolism in Atlantic salmon (Salmo salar L.), the orp...

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Autores principales:	Mónica B Betancor, Elsbeth McStay, Matteo Minghetti, Hervé Migaud, Douglas R Tocher, Andrew Davie
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7ccb27f6c0bf41288466543bd6c41d90
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spelling	oai:doaj.org-article:7ccb27f6c0bf41288466543bd6c41d902021-11-25T06:02:04ZDaily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.).1932-620310.1371/journal.pone.0106739https://doaj.org/article/7ccb27f6c0bf41288466543bd6c41d902014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25184355/?tool=EBIhttps://doaj.org/toc/1932-6203In mammals, several genes involved in liver lipid and cholesterol homeostasis are rhythmically expressed with expression shown to be regulated by clock genes via Rev-erb 1α. In order to elucidate clock gene regulation of genes involved in lipid metabolism in Atlantic salmon (Salmo salar L.), the orphan nuclear receptor Rev-erb 1α was cloned and 24 h expression of clock genes, transcription factors and genes involved in cholesterol and lipid metabolism determined in liver of parr acclimated to a long-day photoperiod, which was previously shown to elicit rhythmic clock gene expression in the brain. Of the 31 genes analysed, significant daily expression was demonstrated in the clock gene Bmal1, transcription factor genes Srebp1, Lxr, Pparα and Pparγ, and several lipid metabolism genes Hmgcr, Ipi, ApoCII and El. The possible regulatory mechanisms and pathways, and the functional significance of these patterns of expression were discussed. Importantly and in contrast to mammals, Per1, Per2, Fas, Srebp2, Cyp71α and Rev-erb 1α did not display significant daily rhythmicity in salmon. The present study is the first report characterising 24 h profiles of gene expression in liver of Atlantic salmon. However, more importantly, the predominant role of lipids in the nutrition and metabolism of fish, and of feed efficiency in determining farming economics, means that daily rhythmicity in the regulation of lipid metabolism will be an area of considerable interest for future research in commercially important species.Mónica B BetancorElsbeth McStayMatteo MinghettiHervé MigaudDouglas R TocherAndrew DaviePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e106739 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mónica B Betancor Elsbeth McStay Matteo Minghetti Hervé Migaud Douglas R Tocher Andrew Davie Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.).
description	In mammals, several genes involved in liver lipid and cholesterol homeostasis are rhythmically expressed with expression shown to be regulated by clock genes via Rev-erb 1α. In order to elucidate clock gene regulation of genes involved in lipid metabolism in Atlantic salmon (Salmo salar L.), the orphan nuclear receptor Rev-erb 1α was cloned and 24 h expression of clock genes, transcription factors and genes involved in cholesterol and lipid metabolism determined in liver of parr acclimated to a long-day photoperiod, which was previously shown to elicit rhythmic clock gene expression in the brain. Of the 31 genes analysed, significant daily expression was demonstrated in the clock gene Bmal1, transcription factor genes Srebp1, Lxr, Pparα and Pparγ, and several lipid metabolism genes Hmgcr, Ipi, ApoCII and El. The possible regulatory mechanisms and pathways, and the functional significance of these patterns of expression were discussed. Importantly and in contrast to mammals, Per1, Per2, Fas, Srebp2, Cyp71α and Rev-erb 1α did not display significant daily rhythmicity in salmon. The present study is the first report characterising 24 h profiles of gene expression in liver of Atlantic salmon. However, more importantly, the predominant role of lipids in the nutrition and metabolism of fish, and of feed efficiency in determining farming economics, means that daily rhythmicity in the regulation of lipid metabolism will be an area of considerable interest for future research in commercially important species.
format	article
author	Mónica B Betancor Elsbeth McStay Matteo Minghetti Hervé Migaud Douglas R Tocher Andrew Davie
author_facet	Mónica B Betancor Elsbeth McStay Matteo Minghetti Hervé Migaud Douglas R Tocher Andrew Davie
author_sort	Mónica B Betancor
title	Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.).
title_short	Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.).
title_full	Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.).
title_fullStr	Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.).
title_full_unstemmed	Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.).
title_sort	daily rhythms in expression of genes of hepatic lipid metabolism in atlantic salmon (salmo salar l.).
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/7ccb27f6c0bf41288466543bd6c41d90
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Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.).

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