Oral immunotherapy for pollen allergy using T-cell epitope-containing egg white derived from genetically manipulated chickens.

Peptide immunotherapy using T-cell epitopes is expected to be an effective treatment for allergic diseases such as Japanese cedar (Cryptomeria japonica; Cj) pollinosis. To develop a treatment for pollen allergy by inducing oral tolerance, we generated genetically manipulated (GM) chickens by retrovi...

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Autores principales:	Yoshinori Kawabe, Yuuki Hayashida, Kensaku Numata, Shota Harada, Yoshifumi Hayashida, Akira Ito, Masamichi Kamihira
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7ccffd6307014eb298f59c2ac1984a54
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spelling	oai:doaj.org-article:7ccffd6307014eb298f59c2ac1984a542021-11-18T08:10:42ZOral immunotherapy for pollen allergy using T-cell epitope-containing egg white derived from genetically manipulated chickens.1932-620310.1371/journal.pone.0048512https://doaj.org/article/7ccffd6307014eb298f59c2ac1984a542012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23144766/?tool=EBIhttps://doaj.org/toc/1932-6203Peptide immunotherapy using T-cell epitopes is expected to be an effective treatment for allergic diseases such as Japanese cedar (Cryptomeria japonica; Cj) pollinosis. To develop a treatment for pollen allergy by inducing oral tolerance, we generated genetically manipulated (GM) chickens by retroviral gene transduction, to produce a fusion protein of chicken egg white lysozyme and a peptide derived from seven dominant human T-cell epitopes of Japanese cedar pollen allergens (cLys-7crp). The transgene sequence was detected in all chickens transduced with the retroviral vector. Transduction efficiency in blood cells correlated to transgene expression. Western blot analysis revealed that cLys-7crp was expressed in the egg white of GM hens. Mice induced to develop allergic rhinitis by Cj pollinosis were fed with cLys-7crp-containing egg white produced by GM chickens. Total and Cj allergen (Cry j 1)-specific IgE levels were significantly decreased in allergic mice fed with cLys-7crp-containing egg white compared with allergic mice fed with normal egg white. These results suggest that oral administration of T-cell epitope-containing egg white derived from GM chickens is effective for the induction of immune tolerance as an allergy therapy.Yoshinori KawabeYuuki HayashidaKensaku NumataShota HaradaYoshifumi HayashidaAkira ItoMasamichi KamihiraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e48512 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yoshinori Kawabe Yuuki Hayashida Kensaku Numata Shota Harada Yoshifumi Hayashida Akira Ito Masamichi Kamihira Oral immunotherapy for pollen allergy using T-cell epitope-containing egg white derived from genetically manipulated chickens.
description	Peptide immunotherapy using T-cell epitopes is expected to be an effective treatment for allergic diseases such as Japanese cedar (Cryptomeria japonica; Cj) pollinosis. To develop a treatment for pollen allergy by inducing oral tolerance, we generated genetically manipulated (GM) chickens by retroviral gene transduction, to produce a fusion protein of chicken egg white lysozyme and a peptide derived from seven dominant human T-cell epitopes of Japanese cedar pollen allergens (cLys-7crp). The transgene sequence was detected in all chickens transduced with the retroviral vector. Transduction efficiency in blood cells correlated to transgene expression. Western blot analysis revealed that cLys-7crp was expressed in the egg white of GM hens. Mice induced to develop allergic rhinitis by Cj pollinosis were fed with cLys-7crp-containing egg white produced by GM chickens. Total and Cj allergen (Cry j 1)-specific IgE levels were significantly decreased in allergic mice fed with cLys-7crp-containing egg white compared with allergic mice fed with normal egg white. These results suggest that oral administration of T-cell epitope-containing egg white derived from GM chickens is effective for the induction of immune tolerance as an allergy therapy.
format	article
author	Yoshinori Kawabe Yuuki Hayashida Kensaku Numata Shota Harada Yoshifumi Hayashida Akira Ito Masamichi Kamihira
author_facet	Yoshinori Kawabe Yuuki Hayashida Kensaku Numata Shota Harada Yoshifumi Hayashida Akira Ito Masamichi Kamihira
author_sort	Yoshinori Kawabe
title	Oral immunotherapy for pollen allergy using T-cell epitope-containing egg white derived from genetically manipulated chickens.
title_short	Oral immunotherapy for pollen allergy using T-cell epitope-containing egg white derived from genetically manipulated chickens.
title_full	Oral immunotherapy for pollen allergy using T-cell epitope-containing egg white derived from genetically manipulated chickens.
title_fullStr	Oral immunotherapy for pollen allergy using T-cell epitope-containing egg white derived from genetically manipulated chickens.
title_full_unstemmed	Oral immunotherapy for pollen allergy using T-cell epitope-containing egg white derived from genetically manipulated chickens.
title_sort	oral immunotherapy for pollen allergy using t-cell epitope-containing egg white derived from genetically manipulated chickens.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/7ccffd6307014eb298f59c2ac1984a54
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Oral immunotherapy for pollen allergy using T-cell epitope-containing egg white derived from genetically manipulated chickens.

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