An in vivo platform to select and evolve aggregation-resistant proteins
Protein aggregation remains a significant challenge for manufacturing of protein biopharmaceuticals. Here, the authors demonstrate the use of directed evolution and an assay for in vivo innate protein aggregation-propensity to generate aggregation-resistant scFv fragments.
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| Lenguaje: | EN |
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Nature Portfolio
2020
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| Acceso en línea: | https://doaj.org/article/7cd4d27ccee2435a841e247a296e334c |
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oai:doaj.org-article:7cd4d27ccee2435a841e247a296e334c2021-12-02T14:40:38ZAn in vivo platform to select and evolve aggregation-resistant proteins10.1038/s41467-020-15667-12041-1723https://doaj.org/article/7cd4d27ccee2435a841e247a296e334c2020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15667-1https://doaj.org/toc/2041-1723Protein aggregation remains a significant challenge for manufacturing of protein biopharmaceuticals. Here, the authors demonstrate the use of directed evolution and an assay for in vivo innate protein aggregation-propensity to generate aggregation-resistant scFv fragments.Jessica S. EboJanet C. SaundersPaul W. A. DevineAlice M. GordonAmy S. WarwickBob SchiffrinStacey E. ChinElizabeth EnglandJames D. ButtonChristopher LloydNicholas J. BondAlison E. AshcroftSheena E. RadfordDavid C. LoweDavid J. BrockwellNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-12 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Jessica S. Ebo Janet C. Saunders Paul W. A. Devine Alice M. Gordon Amy S. Warwick Bob Schiffrin Stacey E. Chin Elizabeth England James D. Button Christopher Lloyd Nicholas J. Bond Alison E. Ashcroft Sheena E. Radford David C. Lowe David J. Brockwell An in vivo platform to select and evolve aggregation-resistant proteins |
| description |
Protein aggregation remains a significant challenge for manufacturing of protein biopharmaceuticals. Here, the authors demonstrate the use of directed evolution and an assay for in vivo innate protein aggregation-propensity to generate aggregation-resistant scFv fragments. |
| format |
article |
| author |
Jessica S. Ebo Janet C. Saunders Paul W. A. Devine Alice M. Gordon Amy S. Warwick Bob Schiffrin Stacey E. Chin Elizabeth England James D. Button Christopher Lloyd Nicholas J. Bond Alison E. Ashcroft Sheena E. Radford David C. Lowe David J. Brockwell |
| author_facet |
Jessica S. Ebo Janet C. Saunders Paul W. A. Devine Alice M. Gordon Amy S. Warwick Bob Schiffrin Stacey E. Chin Elizabeth England James D. Button Christopher Lloyd Nicholas J. Bond Alison E. Ashcroft Sheena E. Radford David C. Lowe David J. Brockwell |
| author_sort |
Jessica S. Ebo |
| title |
An in vivo platform to select and evolve aggregation-resistant proteins |
| title_short |
An in vivo platform to select and evolve aggregation-resistant proteins |
| title_full |
An in vivo platform to select and evolve aggregation-resistant proteins |
| title_fullStr |
An in vivo platform to select and evolve aggregation-resistant proteins |
| title_full_unstemmed |
An in vivo platform to select and evolve aggregation-resistant proteins |
| title_sort |
in vivo platform to select and evolve aggregation-resistant proteins |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/7cd4d27ccee2435a841e247a296e334c |
| work_keys_str_mv |
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