Toxicity and protein composition of venoms of Hottentotta saulcyi, Hottentotta schach and Androctonus crassicauda, three scorpion species collected in Iran

Abstract Background Scorpion stings comprise a serious problem throughout the globe, especially in regions where they are more frequent. Despite a recent upsurge of interest in scorpion venoms by various research groups, there remain many challenges. Objective Therefore, in this study, we aimed to s...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ani Boghozian, Habibollah Nazem, Mohammad Fazilati, Seyed Hossein Hejazi, Mohammadreza Sheikh Sajjadieh
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	albumins amino acids chromatography high pressure liquid lethal dose 50 mice Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/7cdce7d971f44fdda7f7b6af4bb6f8d8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7cdce7d971f44fdda7f7b6af4bb6f8d8
record_format	dspace
spelling	oai:doaj.org-article:7cdce7d971f44fdda7f7b6af4bb6f8d82021-11-19T17:14:25ZToxicity and protein composition of venoms of Hottentotta saulcyi, Hottentotta schach and Androctonus crassicauda, three scorpion species collected in Iran2053-109510.1002/vms3.593https://doaj.org/article/7cdce7d971f44fdda7f7b6af4bb6f8d82021-11-01T00:00:00Zhttps://doi.org/10.1002/vms3.593https://doaj.org/toc/2053-1095Abstract Background Scorpion stings comprise a serious problem throughout the globe, especially in regions where they are more frequent. Despite a recent upsurge of interest in scorpion venoms by various research groups, there remain many challenges. Objective Therefore, in this study, we aimed to study the toxicity and protein composition of venoms of Hottentotta saulcyi, Hottentotta schach and Androctonus crassicauda, three scorpion species collected in Iran. Materials and Methods Scorpion species were collected from Esfahan farm scorpion company and maintained in the laboratory in containers that mimic their natural habitat. Venom was extracted from A. crassicauda, H. schach and H. saulcyi by electrical stimulation of 8 and 10 V. The toxicity of each venom was established by using four groups of male Swiss albino mice aged 2 months (weighting 18–20 g) for testing each dose of venom. One group was used as a control. Venom was injected into mice by subcutaneous route. Then, animals were monitored for 24 h and LD50 was estimated by the graphic method of Miller and Tainter. Thus, high‐performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) method was used to determine amino acids in the venom, and protein concentrations were determined by the Biuret method. Results LD50 of scorpion venoms by subcutaneous route was found to be 1.70 mg/kg b.w (A. crassicauda), 1.47 mg/kg b.w (H. saulcyi) and 0.85 mg/kg b.w (H. schach). A. crassicauda, H. saulcyi and H. schach contain 26, 30, and 31 amino acids, respectively. A. crassicauda contains low concentrations of alpha‐aminoadipic acid, beta‐aminoisobutyric acid, beta‐alanine and citrulline. H. saulcyi contains a concentration of hydroxylysine, whereas H. schach has no such concentration. A. crassicauda also had the highest levels of tyrosine and threonine. Only A. crassicauda venom contains a low proportion of proteins (14.80%) compared with those of H. schach (16.26%) and H. saulcyi (16.20%). Albumin content in the venoms was 11.7% (H. saulcyi), 5.4% (H. schach) and 4.4% (A. crassicauda). Conclusion Scorpions venoms have a variable toxicity and an interesting composition in amino acids and proteins. Work on the development of anti‐venom is fundamental.Ani BoghozianHabibollah NazemMohammad FazilatiSeyed Hossein HejaziMohammadreza Sheikh SajjadiehWileyarticlealbuminsamino acidschromatographyhigh pressure liquidlethal dose 50miceVeterinary medicineSF600-1100ENVeterinary Medicine and Science, Vol 7, Iss 6, Pp 2418-2426 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	albumins amino acids chromatography high pressure liquid lethal dose 50 mice Veterinary medicine SF600-1100
spellingShingle	albumins amino acids chromatography high pressure liquid lethal dose 50 mice Veterinary medicine SF600-1100 Ani Boghozian Habibollah Nazem Mohammad Fazilati Seyed Hossein Hejazi Mohammadreza Sheikh Sajjadieh Toxicity and protein composition of venoms of Hottentotta saulcyi, Hottentotta schach and Androctonus crassicauda, three scorpion species collected in Iran
description	Abstract Background Scorpion stings comprise a serious problem throughout the globe, especially in regions where they are more frequent. Despite a recent upsurge of interest in scorpion venoms by various research groups, there remain many challenges. Objective Therefore, in this study, we aimed to study the toxicity and protein composition of venoms of Hottentotta saulcyi, Hottentotta schach and Androctonus crassicauda, three scorpion species collected in Iran. Materials and Methods Scorpion species were collected from Esfahan farm scorpion company and maintained in the laboratory in containers that mimic their natural habitat. Venom was extracted from A. crassicauda, H. schach and H. saulcyi by electrical stimulation of 8 and 10 V. The toxicity of each venom was established by using four groups of male Swiss albino mice aged 2 months (weighting 18–20 g) for testing each dose of venom. One group was used as a control. Venom was injected into mice by subcutaneous route. Then, animals were monitored for 24 h and LD50 was estimated by the graphic method of Miller and Tainter. Thus, high‐performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) method was used to determine amino acids in the venom, and protein concentrations were determined by the Biuret method. Results LD50 of scorpion venoms by subcutaneous route was found to be 1.70 mg/kg b.w (A. crassicauda), 1.47 mg/kg b.w (H. saulcyi) and 0.85 mg/kg b.w (H. schach). A. crassicauda, H. saulcyi and H. schach contain 26, 30, and 31 amino acids, respectively. A. crassicauda contains low concentrations of alpha‐aminoadipic acid, beta‐aminoisobutyric acid, beta‐alanine and citrulline. H. saulcyi contains a concentration of hydroxylysine, whereas H. schach has no such concentration. A. crassicauda also had the highest levels of tyrosine and threonine. Only A. crassicauda venom contains a low proportion of proteins (14.80%) compared with those of H. schach (16.26%) and H. saulcyi (16.20%). Albumin content in the venoms was 11.7% (H. saulcyi), 5.4% (H. schach) and 4.4% (A. crassicauda). Conclusion Scorpions venoms have a variable toxicity and an interesting composition in amino acids and proteins. Work on the development of anti‐venom is fundamental.
format	article
author	Ani Boghozian Habibollah Nazem Mohammad Fazilati Seyed Hossein Hejazi Mohammadreza Sheikh Sajjadieh
author_facet	Ani Boghozian Habibollah Nazem Mohammad Fazilati Seyed Hossein Hejazi Mohammadreza Sheikh Sajjadieh
author_sort	Ani Boghozian
title	Toxicity and protein composition of venoms of Hottentotta saulcyi, Hottentotta schach and Androctonus crassicauda, three scorpion species collected in Iran
title_short	Toxicity and protein composition of venoms of Hottentotta saulcyi, Hottentotta schach and Androctonus crassicauda, three scorpion species collected in Iran
title_full	Toxicity and protein composition of venoms of Hottentotta saulcyi, Hottentotta schach and Androctonus crassicauda, three scorpion species collected in Iran
title_fullStr	Toxicity and protein composition of venoms of Hottentotta saulcyi, Hottentotta schach and Androctonus crassicauda, three scorpion species collected in Iran
title_full_unstemmed	Toxicity and protein composition of venoms of Hottentotta saulcyi, Hottentotta schach and Androctonus crassicauda, three scorpion species collected in Iran
title_sort	toxicity and protein composition of venoms of hottentotta saulcyi, hottentotta schach and androctonus crassicauda, three scorpion species collected in iran
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/7cdce7d971f44fdda7f7b6af4bb6f8d8
work_keys_str_mv	AT aniboghozian toxicityandproteincompositionofvenomsofhottentottasaulcyihottentottaschachandandroctonuscrassicaudathreescorpionspeciescollectediniran AT habibollahnazem toxicityandproteincompositionofvenomsofhottentottasaulcyihottentottaschachandandroctonuscrassicaudathreescorpionspeciescollectediniran AT mohammadfazilati toxicityandproteincompositionofvenomsofhottentottasaulcyihottentottaschachandandroctonuscrassicaudathreescorpionspeciescollectediniran AT seyedhosseinhejazi toxicityandproteincompositionofvenomsofhottentottasaulcyihottentottaschachandandroctonuscrassicaudathreescorpionspeciescollectediniran AT mohammadrezasheikhsajjadieh toxicityandproteincompositionofvenomsofhottentottasaulcyihottentottaschachandandroctonuscrassicaudathreescorpionspeciescollectediniran
_version_	1718420021538979840

Toxicity and protein composition of venoms of Hottentotta saulcyi, Hottentotta schach and Androctonus crassicauda, three scorpion species collected in Iran

Ejemplares similares