Refining the mandibular osteoradionecrosis rat model by in vivo longitudinal µCT analysis

Abstract Osteoradionecrosis (ORN) is one of the most feared side effects of radiotherapy following cancers of the upper aero-digestive tract and leading to severe functional defects in patients. Today, our lack of knowledge about the physiopathology restricts the development of new treatments. In th...

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Autores principales:	Morgane Dos Santos, Christelle Demarquay, Louis Ermeneux, Fazia Aberkane, Pauline Bléry, Pierre Weiss, Fabien Milliat, Noëlle Mathieu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7ce266c647024817a99460401b686278
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spelling	oai:doaj.org-article:7ce266c647024817a99460401b6862782021-11-21T12:17:44ZRefining the mandibular osteoradionecrosis rat model by in vivo longitudinal µCT analysis10.1038/s41598-021-01229-y2045-2322https://doaj.org/article/7ce266c647024817a99460401b6862782021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01229-yhttps://doaj.org/toc/2045-2322Abstract Osteoradionecrosis (ORN) is one of the most feared side effects of radiotherapy following cancers of the upper aero-digestive tract and leading to severe functional defects in patients. Today, our lack of knowledge about the physiopathology restricts the development of new treatments. In this study, we refined the ORN rat model and quantitatively studied the progression of the disease. We tested the impact of radiation doses from 20 to 40 Gy, delivered with incident 4MV X-ray beams on the left mandible of the inbred Lewis Rat. We used micro-computed tomography (µCT) to obtain in vivo images for longitudinal bone imaging and ex vivo images after animal perfusion with barium sulphate contrast agent for vessel imaging. We compared quantification methods by analyzing 3D images and 2D measurements to determine the most appropriate and precise method according to the degree of damage. We defined 25 Gy as the minimum irradiation dose combined with the median molar extraction necessary to develop non-regenerative bone necrosis. µCT image analyses were correlated with clinical and histological analyses. This refined model and accurate methods for bone and vessel quantification will improve our knowledge of the progression of ORN pathology and allow us to test the efficacy of new regenerative medicine procedures.Morgane Dos SantosChristelle DemarquayLouis ErmeneuxFazia AberkanePauline BléryPierre WeissFabien MilliatNoëlle MathieuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Morgane Dos Santos Christelle Demarquay Louis Ermeneux Fazia Aberkane Pauline Bléry Pierre Weiss Fabien Milliat Noëlle Mathieu Refining the mandibular osteoradionecrosis rat model by in vivo longitudinal µCT analysis
description	Abstract Osteoradionecrosis (ORN) is one of the most feared side effects of radiotherapy following cancers of the upper aero-digestive tract and leading to severe functional defects in patients. Today, our lack of knowledge about the physiopathology restricts the development of new treatments. In this study, we refined the ORN rat model and quantitatively studied the progression of the disease. We tested the impact of radiation doses from 20 to 40 Gy, delivered with incident 4MV X-ray beams on the left mandible of the inbred Lewis Rat. We used micro-computed tomography (µCT) to obtain in vivo images for longitudinal bone imaging and ex vivo images after animal perfusion with barium sulphate contrast agent for vessel imaging. We compared quantification methods by analyzing 3D images and 2D measurements to determine the most appropriate and precise method according to the degree of damage. We defined 25 Gy as the minimum irradiation dose combined with the median molar extraction necessary to develop non-regenerative bone necrosis. µCT image analyses were correlated with clinical and histological analyses. This refined model and accurate methods for bone and vessel quantification will improve our knowledge of the progression of ORN pathology and allow us to test the efficacy of new regenerative medicine procedures.
format	article
author	Morgane Dos Santos Christelle Demarquay Louis Ermeneux Fazia Aberkane Pauline Bléry Pierre Weiss Fabien Milliat Noëlle Mathieu
author_facet	Morgane Dos Santos Christelle Demarquay Louis Ermeneux Fazia Aberkane Pauline Bléry Pierre Weiss Fabien Milliat Noëlle Mathieu
author_sort	Morgane Dos Santos
title	Refining the mandibular osteoradionecrosis rat model by in vivo longitudinal µCT analysis
title_short	Refining the mandibular osteoradionecrosis rat model by in vivo longitudinal µCT analysis
title_full	Refining the mandibular osteoradionecrosis rat model by in vivo longitudinal µCT analysis
title_fullStr	Refining the mandibular osteoradionecrosis rat model by in vivo longitudinal µCT analysis
title_full_unstemmed	Refining the mandibular osteoradionecrosis rat model by in vivo longitudinal µCT analysis
title_sort	refining the mandibular osteoradionecrosis rat model by in vivo longitudinal µct analysis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7ce266c647024817a99460401b686278
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Refining the mandibular osteoradionecrosis rat model by in vivo longitudinal µCT analysis

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