A multiplexed circulating tumor DNA detection platform engineered from 3D-coded interlocked DNA rings

Circulating tumor DNA (ctDNA) is a critical biomarker not only important for the early detection of tumors but also invaluable for personalized treatments. Currently ctDNA detection relies on sequencing. Here, a platform termed three-dimensional-coded interlocked DNA rings (3D-coded ID rings) was cr...

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Autores principales:	Sha Yang, Xinyu Zhan, Xiaoqi Tang, Shuang Zhao, Lianyu Yu, Mingxuan Gao, Dan Luo, Yunxia Wang, Kai Chang, Ming Chen
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	3D-coded ID rings Multiplexed detection Circulating tumor DNA Colorectal cancer Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7ce39fdac8474016893ea4b362096862
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spelling	oai:doaj.org-article:7ce39fdac8474016893ea4b3620968622021-11-26T04:37:06ZA multiplexed circulating tumor DNA detection platform engineered from 3D-coded interlocked DNA rings2452-199X10.1016/j.bioactmat.2021.09.007https://doaj.org/article/7ce39fdac8474016893ea4b3620968622022-04-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2452199X21004175https://doaj.org/toc/2452-199XCirculating tumor DNA (ctDNA) is a critical biomarker not only important for the early detection of tumors but also invaluable for personalized treatments. Currently ctDNA detection relies on sequencing. Here, a platform termed three-dimensional-coded interlocked DNA rings (3D-coded ID rings) was created for multiplexed ctDNA identification. The ID rings provide a ctDNA recognition ring that is physically interlocked with a reporter ring. The specific binding of ctDNA to the recognition ring initiates target-responsive cutting via a restriction endonuclease; the cutting then triggers rolling circle amplification on the reporter ring. The signals are further integrated with internal 3D codes for multiplexed readouts. ctDNAs from non-invasive clinical specimens including plasma, feces, and urine were detected and validated at a sensitivity much higher than those obtained through sequencing. This 3D-coded ID ring platform can detect any multiple DNA fragments simultaneously without sequencing. We envision that our platform will facilitate the implementation of future personalized/precision medicine.Sha YangXinyu ZhanXiaoqi TangShuang ZhaoLianyu YuMingxuan GaoDan LuoYunxia WangKai ChangMing ChenKeAi Communications Co., Ltd.article3D-coded ID ringsMultiplexed detectionCirculating tumor DNAColorectal cancerMaterials of engineering and construction. Mechanics of materialsTA401-492Biology (General)QH301-705.5ENBioactive Materials, Vol 10, Iss , Pp 68-78 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	3D-coded ID rings Multiplexed detection Circulating tumor DNA Colorectal cancer Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
spellingShingle	3D-coded ID rings Multiplexed detection Circulating tumor DNA Colorectal cancer Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5 Sha Yang Xinyu Zhan Xiaoqi Tang Shuang Zhao Lianyu Yu Mingxuan Gao Dan Luo Yunxia Wang Kai Chang Ming Chen A multiplexed circulating tumor DNA detection platform engineered from 3D-coded interlocked DNA rings
description	Circulating tumor DNA (ctDNA) is a critical biomarker not only important for the early detection of tumors but also invaluable for personalized treatments. Currently ctDNA detection relies on sequencing. Here, a platform termed three-dimensional-coded interlocked DNA rings (3D-coded ID rings) was created for multiplexed ctDNA identification. The ID rings provide a ctDNA recognition ring that is physically interlocked with a reporter ring. The specific binding of ctDNA to the recognition ring initiates target-responsive cutting via a restriction endonuclease; the cutting then triggers rolling circle amplification on the reporter ring. The signals are further integrated with internal 3D codes for multiplexed readouts. ctDNAs from non-invasive clinical specimens including plasma, feces, and urine were detected and validated at a sensitivity much higher than those obtained through sequencing. This 3D-coded ID ring platform can detect any multiple DNA fragments simultaneously without sequencing. We envision that our platform will facilitate the implementation of future personalized/precision medicine.
format	article
author	Sha Yang Xinyu Zhan Xiaoqi Tang Shuang Zhao Lianyu Yu Mingxuan Gao Dan Luo Yunxia Wang Kai Chang Ming Chen
author_facet	Sha Yang Xinyu Zhan Xiaoqi Tang Shuang Zhao Lianyu Yu Mingxuan Gao Dan Luo Yunxia Wang Kai Chang Ming Chen
author_sort	Sha Yang
title	A multiplexed circulating tumor DNA detection platform engineered from 3D-coded interlocked DNA rings
title_short	A multiplexed circulating tumor DNA detection platform engineered from 3D-coded interlocked DNA rings
title_full	A multiplexed circulating tumor DNA detection platform engineered from 3D-coded interlocked DNA rings
title_fullStr	A multiplexed circulating tumor DNA detection platform engineered from 3D-coded interlocked DNA rings
title_full_unstemmed	A multiplexed circulating tumor DNA detection platform engineered from 3D-coded interlocked DNA rings
title_sort	multiplexed circulating tumor dna detection platform engineered from 3d-coded interlocked dna rings
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/7ce39fdac8474016893ea4b362096862
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A multiplexed circulating tumor DNA detection platform engineered from 3D-coded interlocked DNA rings

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