Functional selection of shRNA loops from randomized retroviral libraries.
Gene silencing by RNA interference (RNAi) can be achieved by the ectopic expression of tailored short hairpin RNAs (shRNAs) which after export to the cytoplasm are processed by Dicer and incorporated into the RNA induced silencing complex (RISC). Design rules for shRNAs have been the focus of severa...
Guardado en:
Autores principales: | Stig Mølgaard Rask Jensen, Alexander Schmitz, Finn Skou Pedersen, Jørgen Kjems, Jesper Bertram Bramsen |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2012
|
Materias: | |
Acceso en línea: | https://doaj.org/article/7ce8f2ace8eb476f81eee2ca98e8ccb5 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
Ejemplares similares
-
Optimized PCR conditions and increased shRNA fold representation improve reproducibility of pooled shRNA screens.
por: Žaklina Strezoska, et al.
Publicado: (2012) -
Re-inspection of small RNA sequence datasets reveals several novel human miRNA genes.
por: Thomas Birkballe Hansen, et al.
Publicado: (2010) -
shRNA transgenic swine display resistance to infection with the foot-and-mouth disease virus
por: Wenping Hu, et al.
Publicado: (2021) -
An infinitely expandable cloning strategy plus repeat-proof PCR for working with multiple shRNA.
por: Glen John McIntyre, et al.
Publicado: (2008) -
Cationic vesicles for efficient shRNA transfection in the MCF-7 breast cancer cell line
por: Mokhtary P, et al.
Publicado: (2018)