Transcriptional control of motility enables directional movement of Escherichia coli in a signal gradient

Abstract Manipulation of cellular motility using a target signal can facilitate the development of biosensors or microbe-powered biorobots. Here, we engineered signal-dependent motility in Escherichia coli via the transcriptional control of a key motility gene. Without manipulating chemotaxis, signa...

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Autores principales:	Jayamary Divya Ravichandar, Adam G. Bower, A. Agung Julius, Cynthia H. Collins
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7ce974fb4c5e404380f246abf87b88f6
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spelling	oai:doaj.org-article:7ce974fb4c5e404380f246abf87b88f62021-12-02T15:05:29ZTranscriptional control of motility enables directional movement of Escherichia coli in a signal gradient10.1038/s41598-017-08870-62045-2322https://doaj.org/article/7ce974fb4c5e404380f246abf87b88f62017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08870-6https://doaj.org/toc/2045-2322Abstract Manipulation of cellular motility using a target signal can facilitate the development of biosensors or microbe-powered biorobots. Here, we engineered signal-dependent motility in Escherichia coli via the transcriptional control of a key motility gene. Without manipulating chemotaxis, signal-dependent switching of motility, either on or off, led to population-level directional movement of cells up or down a signal gradient. We developed a mathematical model that captures the behaviour of the cells, enables identification of key parameters controlling system behaviour, and facilitates predictive-design of motility-based pattern formation. We demonstrated that motility of the receiver strains could be controlled by a sender strain generating a signal gradient. The modular quorum sensing-dependent architecture for interfacing different senders with receivers enabled a broad range of systems-level behaviours. The directional control of motility, especially combined with the potential to incorporate tuneable sensors and more complex sensing-logic, may lead to tools for novel biosensing and targeted-delivery applications.Jayamary Divya RavichandarAdam G. BowerA. Agung JuliusCynthia H. CollinsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jayamary Divya Ravichandar Adam G. Bower A. Agung Julius Cynthia H. Collins Transcriptional control of motility enables directional movement of Escherichia coli in a signal gradient
description	Abstract Manipulation of cellular motility using a target signal can facilitate the development of biosensors or microbe-powered biorobots. Here, we engineered signal-dependent motility in Escherichia coli via the transcriptional control of a key motility gene. Without manipulating chemotaxis, signal-dependent switching of motility, either on or off, led to population-level directional movement of cells up or down a signal gradient. We developed a mathematical model that captures the behaviour of the cells, enables identification of key parameters controlling system behaviour, and facilitates predictive-design of motility-based pattern formation. We demonstrated that motility of the receiver strains could be controlled by a sender strain generating a signal gradient. The modular quorum sensing-dependent architecture for interfacing different senders with receivers enabled a broad range of systems-level behaviours. The directional control of motility, especially combined with the potential to incorporate tuneable sensors and more complex sensing-logic, may lead to tools for novel biosensing and targeted-delivery applications.
format	article
author	Jayamary Divya Ravichandar Adam G. Bower A. Agung Julius Cynthia H. Collins
author_facet	Jayamary Divya Ravichandar Adam G. Bower A. Agung Julius Cynthia H. Collins
author_sort	Jayamary Divya Ravichandar
title	Transcriptional control of motility enables directional movement of Escherichia coli in a signal gradient
title_short	Transcriptional control of motility enables directional movement of Escherichia coli in a signal gradient
title_full	Transcriptional control of motility enables directional movement of Escherichia coli in a signal gradient
title_fullStr	Transcriptional control of motility enables directional movement of Escherichia coli in a signal gradient
title_full_unstemmed	Transcriptional control of motility enables directional movement of Escherichia coli in a signal gradient
title_sort	transcriptional control of motility enables directional movement of escherichia coli in a signal gradient
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7ce974fb4c5e404380f246abf87b88f6
work_keys_str_mv	AT jayamarydivyaravichandar transcriptionalcontrolofmotilityenablesdirectionalmovementofescherichiacoliinasignalgradient AT adamgbower transcriptionalcontrolofmotilityenablesdirectionalmovementofescherichiacoliinasignalgradient AT aagungjulius transcriptionalcontrolofmotilityenablesdirectionalmovementofescherichiacoliinasignalgradient AT cynthiahcollins transcriptionalcontrolofmotilityenablesdirectionalmovementofescherichiacoliinasignalgradient
_version_	1718388841051586560

Transcriptional control of motility enables directional movement of Escherichia coli in a signal gradient

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