Bioengineered 3D nanocomposite based on gold nanoparticles and gelatin nanofibers for bone regeneration: in vitro and in vivo study

Abstract The main aim of the present study was to fabricate 3D scaffold based on poly (l-lactic acid) (PLLA)/Polycaprolactone (PCL) matrix polymer containing gelatin nanofibers (GNFs) and gold nanoparticles (AuNPs) as the scaffold for bone tissue engineering application. AuNPs were synthesized via t...

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Autores principales:	Hadi Samadian, Hossein Khastar, Arian Ehterami, Majid Salehi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7cef082e69d941cbaf327c253d3ac1f7
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spelling	oai:doaj.org-article:7cef082e69d941cbaf327c253d3ac1f72021-12-02T15:39:50ZBioengineered 3D nanocomposite based on gold nanoparticles and gelatin nanofibers for bone regeneration: in vitro and in vivo study10.1038/s41598-021-93367-62045-2322https://doaj.org/article/7cef082e69d941cbaf327c253d3ac1f72021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93367-6https://doaj.org/toc/2045-2322Abstract The main aim of the present study was to fabricate 3D scaffold based on poly (l-lactic acid) (PLLA)/Polycaprolactone (PCL) matrix polymer containing gelatin nanofibers (GNFs) and gold nanoparticles (AuNPs) as the scaffold for bone tissue engineering application. AuNPs were synthesized via the Turkevich method as the osteogenic factor. GNFs were fabricated by the electrospinning methods and implemented into the scaffold as the extracellular matrix mimicry structure. The prepared AuNPs and Gel nanofibers were composited by PLLA/PCL matrix polymer and converted to a 3D scaffold using thermal-induced phase separation. SEM imaging illustrated the scaffold's porous structure with a porosity range of 80–90% and a pore size range of 80 to 130 µm. The in vitro studies showed that the highest concentration of AuNPs (160 ppm) induced toxicity and 80 ppm AuNPs exhibited the highest cell proliferation. The in vivo studies showed that PCL/PLLA/Gel/80ppmAuNPs induced the highest neo-bone formation, osteocyte in lacuna woven bone formation, and angiogenesis in the defect site. In conclusion, this study showed that the prepared scaffold exhibited suitable properties for bone tissue engineering in terms of porosity, pore size, mechanical properties, biocompatibility, and osteoconduction activities.Hadi SamadianHossein KhastarArian EhteramiMajid SalehiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hadi Samadian Hossein Khastar Arian Ehterami Majid Salehi Bioengineered 3D nanocomposite based on gold nanoparticles and gelatin nanofibers for bone regeneration: in vitro and in vivo study
description	Abstract The main aim of the present study was to fabricate 3D scaffold based on poly (l-lactic acid) (PLLA)/Polycaprolactone (PCL) matrix polymer containing gelatin nanofibers (GNFs) and gold nanoparticles (AuNPs) as the scaffold for bone tissue engineering application. AuNPs were synthesized via the Turkevich method as the osteogenic factor. GNFs were fabricated by the electrospinning methods and implemented into the scaffold as the extracellular matrix mimicry structure. The prepared AuNPs and Gel nanofibers were composited by PLLA/PCL matrix polymer and converted to a 3D scaffold using thermal-induced phase separation. SEM imaging illustrated the scaffold's porous structure with a porosity range of 80–90% and a pore size range of 80 to 130 µm. The in vitro studies showed that the highest concentration of AuNPs (160 ppm) induced toxicity and 80 ppm AuNPs exhibited the highest cell proliferation. The in vivo studies showed that PCL/PLLA/Gel/80ppmAuNPs induced the highest neo-bone formation, osteocyte in lacuna woven bone formation, and angiogenesis in the defect site. In conclusion, this study showed that the prepared scaffold exhibited suitable properties for bone tissue engineering in terms of porosity, pore size, mechanical properties, biocompatibility, and osteoconduction activities.
format	article
author	Hadi Samadian Hossein Khastar Arian Ehterami Majid Salehi
author_facet	Hadi Samadian Hossein Khastar Arian Ehterami Majid Salehi
author_sort	Hadi Samadian
title	Bioengineered 3D nanocomposite based on gold nanoparticles and gelatin nanofibers for bone regeneration: in vitro and in vivo study
title_short	Bioengineered 3D nanocomposite based on gold nanoparticles and gelatin nanofibers for bone regeneration: in vitro and in vivo study
title_full	Bioengineered 3D nanocomposite based on gold nanoparticles and gelatin nanofibers for bone regeneration: in vitro and in vivo study
title_fullStr	Bioengineered 3D nanocomposite based on gold nanoparticles and gelatin nanofibers for bone regeneration: in vitro and in vivo study
title_full_unstemmed	Bioengineered 3D nanocomposite based on gold nanoparticles and gelatin nanofibers for bone regeneration: in vitro and in vivo study
title_sort	bioengineered 3d nanocomposite based on gold nanoparticles and gelatin nanofibers for bone regeneration: in vitro and in vivo study
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7cef082e69d941cbaf327c253d3ac1f7
work_keys_str_mv	AT hadisamadian bioengineered3dnanocompositebasedongoldnanoparticlesandgelatinnanofibersforboneregenerationinvitroandinvivostudy AT hosseinkhastar bioengineered3dnanocompositebasedongoldnanoparticlesandgelatinnanofibersforboneregenerationinvitroandinvivostudy AT arianehterami bioengineered3dnanocompositebasedongoldnanoparticlesandgelatinnanofibersforboneregenerationinvitroandinvivostudy AT majidsalehi bioengineered3dnanocompositebasedongoldnanoparticlesandgelatinnanofibersforboneregenerationinvitroandinvivostudy
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Bioengineered 3D nanocomposite based on gold nanoparticles and gelatin nanofibers for bone regeneration: in vitro and in vivo study

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