Real-time in vivo two-photon imaging study reveals decreased cerebro-vascular volume and increased blood-brain barrier permeability in chronically stressed mice

Abstract Chronic stress disrupts brain homeostasis and adversely affects the cerebro-vascular system. Even though the effects of chronic stress on brain system have been extensively studied, there are few in vivo dynamic studies on the effects of chronic stress on the cerebro-vascular system. In thi...

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Autores principales:	Sohee Lee, Bok-Man Kang, Jae Hwan Kim, Jiwoong Min, Hyung Seok Kim, Hyunwoo Ryu, Hyejin Park, Sungjun Bae, Daehwan Oh, Myunghwan Choi, Minah Suh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7cf3751edc2d428a9474931de36fe6df
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spelling	oai:doaj.org-article:7cf3751edc2d428a9474931de36fe6df2021-12-02T15:08:56ZReal-time in vivo two-photon imaging study reveals decreased cerebro-vascular volume and increased blood-brain barrier permeability in chronically stressed mice10.1038/s41598-018-30875-y2045-2322https://doaj.org/article/7cf3751edc2d428a9474931de36fe6df2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30875-yhttps://doaj.org/toc/2045-2322Abstract Chronic stress disrupts brain homeostasis and adversely affects the cerebro-vascular system. Even though the effects of chronic stress on brain system have been extensively studied, there are few in vivo dynamic studies on the effects of chronic stress on the cerebro-vascular system. In this study, the effects of chronic stress on cerebral vasculature and BBB permeability were studied using in vivo two-photon (2p) microscopic imaging with an injection of fluorescence-conjugated dextran. Our real-time 2p imaging results showed that chronic stress reduced the vessel diameter and reconstructed vascular volume, regardless of vessel type and branching order. BBB permeability was investigated with two different size of tracers. Stressed animals exhibited a greater BBB permeability to 40-kDa dextran, but not to 70-kDa dextran, which is suggestive of weakened vascular integrity following stress. Molecular analysis revealed significantly higher VEGFa mRNA expression and a reduction in claudin-5. In summary, chronic stress decreases the size of cerebral vessels and increases BBB permeability. These results may suggest that the sustained decrease in cerebro-vascular volume due to chronic stress leads to a hypoxic condition that causes molecular changes such as VEGF and claudin-5, which eventually impairs the function of BBB.Sohee LeeBok-Man KangJae Hwan KimJiwoong MinHyung Seok KimHyunwoo RyuHyejin ParkSungjun BaeDaehwan OhMyunghwan ChoiMinah SuhNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sohee Lee Bok-Man Kang Jae Hwan Kim Jiwoong Min Hyung Seok Kim Hyunwoo Ryu Hyejin Park Sungjun Bae Daehwan Oh Myunghwan Choi Minah Suh Real-time in vivo two-photon imaging study reveals decreased cerebro-vascular volume and increased blood-brain barrier permeability in chronically stressed mice
description	Abstract Chronic stress disrupts brain homeostasis and adversely affects the cerebro-vascular system. Even though the effects of chronic stress on brain system have been extensively studied, there are few in vivo dynamic studies on the effects of chronic stress on the cerebro-vascular system. In this study, the effects of chronic stress on cerebral vasculature and BBB permeability were studied using in vivo two-photon (2p) microscopic imaging with an injection of fluorescence-conjugated dextran. Our real-time 2p imaging results showed that chronic stress reduced the vessel diameter and reconstructed vascular volume, regardless of vessel type and branching order. BBB permeability was investigated with two different size of tracers. Stressed animals exhibited a greater BBB permeability to 40-kDa dextran, but not to 70-kDa dextran, which is suggestive of weakened vascular integrity following stress. Molecular analysis revealed significantly higher VEGFa mRNA expression and a reduction in claudin-5. In summary, chronic stress decreases the size of cerebral vessels and increases BBB permeability. These results may suggest that the sustained decrease in cerebro-vascular volume due to chronic stress leads to a hypoxic condition that causes molecular changes such as VEGF and claudin-5, which eventually impairs the function of BBB.
format	article
author	Sohee Lee Bok-Man Kang Jae Hwan Kim Jiwoong Min Hyung Seok Kim Hyunwoo Ryu Hyejin Park Sungjun Bae Daehwan Oh Myunghwan Choi Minah Suh
author_facet	Sohee Lee Bok-Man Kang Jae Hwan Kim Jiwoong Min Hyung Seok Kim Hyunwoo Ryu Hyejin Park Sungjun Bae Daehwan Oh Myunghwan Choi Minah Suh
author_sort	Sohee Lee
title	Real-time in vivo two-photon imaging study reveals decreased cerebro-vascular volume and increased blood-brain barrier permeability in chronically stressed mice
title_short	Real-time in vivo two-photon imaging study reveals decreased cerebro-vascular volume and increased blood-brain barrier permeability in chronically stressed mice
title_full	Real-time in vivo two-photon imaging study reveals decreased cerebro-vascular volume and increased blood-brain barrier permeability in chronically stressed mice
title_fullStr	Real-time in vivo two-photon imaging study reveals decreased cerebro-vascular volume and increased blood-brain barrier permeability in chronically stressed mice
title_full_unstemmed	Real-time in vivo two-photon imaging study reveals decreased cerebro-vascular volume and increased blood-brain barrier permeability in chronically stressed mice
title_sort	real-time in vivo two-photon imaging study reveals decreased cerebro-vascular volume and increased blood-brain barrier permeability in chronically stressed mice
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/7cf3751edc2d428a9474931de36fe6df
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Real-time in vivo two-photon imaging study reveals decreased cerebro-vascular volume and increased blood-brain barrier permeability in chronically stressed mice

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