ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces

Yamal Al-ramahi et al. describe an accelerated laboratory evolution method that applies ssDNA recombineering and bacterial surface display to engineer nanobody epitope recognition. As a proof-of-concept, they evolved TD4 recognition to bind the translocated intimin receptor (TirM) of EPEC pathogens,...

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Autores principales:	Yamal Al-ramahi, Akos Nyerges, Yago Margolles, Lidia Cerdán, Gyorgyi Ferenc, Csaba Pál, Luis Ángel Fernández, Víctor de Lorenzo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7cfb63c37a134a1381dcf213cbee4ff5
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spelling	oai:doaj.org-article:7cfb63c37a134a1381dcf213cbee4ff52021-12-02T18:01:38ZssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces10.1038/s42003-021-02702-02399-3642https://doaj.org/article/7cfb63c37a134a1381dcf213cbee4ff52021-10-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02702-0https://doaj.org/toc/2399-3642Yamal Al-ramahi et al. describe an accelerated laboratory evolution method that applies ssDNA recombineering and bacterial surface display to engineer nanobody epitope recognition. As a proof-of-concept, they evolved TD4 recognition to bind the translocated intimin receptor (TirM) of EPEC pathogens, with this method bypassing the need for laborious cloning and mutagenesis procedures.Yamal Al-ramahiAkos NyergesYago MargollesLidia CerdánGyorgyi FerencCsaba PálLuis Ángel FernándezVíctor de LorenzoNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Yamal Al-ramahi Akos Nyerges Yago Margolles Lidia Cerdán Gyorgyi Ferenc Csaba Pál Luis Ángel Fernández Víctor de Lorenzo ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces
description	Yamal Al-ramahi et al. describe an accelerated laboratory evolution method that applies ssDNA recombineering and bacterial surface display to engineer nanobody epitope recognition. As a proof-of-concept, they evolved TD4 recognition to bind the translocated intimin receptor (TirM) of EPEC pathogens, with this method bypassing the need for laborious cloning and mutagenesis procedures.
format	article
author	Yamal Al-ramahi Akos Nyerges Yago Margolles Lidia Cerdán Gyorgyi Ferenc Csaba Pál Luis Ángel Fernández Víctor de Lorenzo
author_facet	Yamal Al-ramahi Akos Nyerges Yago Margolles Lidia Cerdán Gyorgyi Ferenc Csaba Pál Luis Ángel Fernández Víctor de Lorenzo
author_sort	Yamal Al-ramahi
title	ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces
title_short	ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces
title_full	ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces
title_fullStr	ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces
title_full_unstemmed	ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces
title_sort	ssdna recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7cfb63c37a134a1381dcf213cbee4ff5
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ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces

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