Optimization of Murrayafoline A ethanol extraction process from the roots of Glycosmis stenocarpa, and evaluation of its Tumorigenesis inhibition activity on Hep-G2 cells

Glycosmis stenocarpa is a species of shrub found in the Northern provinces of Vietnam. Its roots contain different carbazolic derivatives, mainly Murrayafoline A (Mu-A), which exhibits valuable biological activities. In this study, we performed an extraction of Mu-A from the roots of G. stenocarpa a...

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Autores principales:	Tran Quoc Toan, Pham The Dan, Nguyen Thanh Duong, Luu Van Huyen, Nghi Do Huu, Duy Le Xuan, Nguyen Phi Hung, Nguyen Manh Cuong, Luu Van Chinh, Pham Minh Quan, Huyen Vu Thi, Pham Tri Nhut, Pham Dung Thuy Nguyen
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	mu-a g. stenocarpa antitumor-promoting activity hep-g2 optimization Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/7cfc32f2b84f46349ade41bc2817f222
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spelling	oai:doaj.org-article:7cfc32f2b84f46349ade41bc2817f2222021-12-05T14:10:44ZOptimization of Murrayafoline A ethanol extraction process from the roots of Glycosmis stenocarpa, and evaluation of its Tumorigenesis inhibition activity on Hep-G2 cells2391-542010.1515/chem-2021-0067https://doaj.org/article/7cfc32f2b84f46349ade41bc2817f2222021-07-01T00:00:00Zhttps://doi.org/10.1515/chem-2021-0067https://doaj.org/toc/2391-5420Glycosmis stenocarpa is a species of shrub found in the Northern provinces of Vietnam. Its roots contain different carbazolic derivatives, mainly Murrayafoline A (Mu-A), which exhibits valuable biological activities. In this study, we performed an extraction of Mu-A from the roots of G. stenocarpa and optimized this process using response surface methodology (RSM) according to a central composite design, with three independent parameters including extraction time (min), extraction temperature (°C), and solvent/material ratio (mL/g). Two dependent variables were the Mu-A content (mg/g raw materials) and extraction efficiency (%). The optimal conditions to extract Mu-A were found to be as follows: extraction temperature, 67°C; extraction time, 165 min; and solvent/material ratio, 5:1. Under these conditions, the Mu-A content and extraction efficiency were 38.94 ± 1.31 mg/g raw materials and 34.98 ± 1.18%, respectively. Mu-A exhibited antiproliferation and antitumor-promoting activity against the HepG-2 cell line. The present optimization work of Mu-A extraction from G. stenocarpa roots contributed to the attempt of designing a large-scale extraction process for the compound and further exploitation of its potential in vivo applications.Tran Quoc ToanPham The DanNguyen Thanh DuongLuu Van HuyenNghi Do HuuDuy Le XuanNguyen Phi HungNguyen Manh CuongLuu Van ChinhPham Minh QuanHuyen Vu ThiPham Tri NhutPham Dung Thuy NguyenDe Gruyterarticlemu-ag. stenocarpaantitumor-promoting activityhep-g2optimizationChemistryQD1-999ENOpen Chemistry, Vol 19, Iss 1, Pp 830-842 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mu-a g. stenocarpa antitumor-promoting activity hep-g2 optimization Chemistry QD1-999
spellingShingle	mu-a g. stenocarpa antitumor-promoting activity hep-g2 optimization Chemistry QD1-999 Tran Quoc Toan Pham The Dan Nguyen Thanh Duong Luu Van Huyen Nghi Do Huu Duy Le Xuan Nguyen Phi Hung Nguyen Manh Cuong Luu Van Chinh Pham Minh Quan Huyen Vu Thi Pham Tri Nhut Pham Dung Thuy Nguyen Optimization of Murrayafoline A ethanol extraction process from the roots of Glycosmis stenocarpa, and evaluation of its Tumorigenesis inhibition activity on Hep-G2 cells
description	Glycosmis stenocarpa is a species of shrub found in the Northern provinces of Vietnam. Its roots contain different carbazolic derivatives, mainly Murrayafoline A (Mu-A), which exhibits valuable biological activities. In this study, we performed an extraction of Mu-A from the roots of G. stenocarpa and optimized this process using response surface methodology (RSM) according to a central composite design, with three independent parameters including extraction time (min), extraction temperature (°C), and solvent/material ratio (mL/g). Two dependent variables were the Mu-A content (mg/g raw materials) and extraction efficiency (%). The optimal conditions to extract Mu-A were found to be as follows: extraction temperature, 67°C; extraction time, 165 min; and solvent/material ratio, 5:1. Under these conditions, the Mu-A content and extraction efficiency were 38.94 ± 1.31 mg/g raw materials and 34.98 ± 1.18%, respectively. Mu-A exhibited antiproliferation and antitumor-promoting activity against the HepG-2 cell line. The present optimization work of Mu-A extraction from G. stenocarpa roots contributed to the attempt of designing a large-scale extraction process for the compound and further exploitation of its potential in vivo applications.
format	article
author	Tran Quoc Toan Pham The Dan Nguyen Thanh Duong Luu Van Huyen Nghi Do Huu Duy Le Xuan Nguyen Phi Hung Nguyen Manh Cuong Luu Van Chinh Pham Minh Quan Huyen Vu Thi Pham Tri Nhut Pham Dung Thuy Nguyen
author_facet	Tran Quoc Toan Pham The Dan Nguyen Thanh Duong Luu Van Huyen Nghi Do Huu Duy Le Xuan Nguyen Phi Hung Nguyen Manh Cuong Luu Van Chinh Pham Minh Quan Huyen Vu Thi Pham Tri Nhut Pham Dung Thuy Nguyen
author_sort	Tran Quoc Toan
title	Optimization of Murrayafoline A ethanol extraction process from the roots of Glycosmis stenocarpa, and evaluation of its Tumorigenesis inhibition activity on Hep-G2 cells
title_short	Optimization of Murrayafoline A ethanol extraction process from the roots of Glycosmis stenocarpa, and evaluation of its Tumorigenesis inhibition activity on Hep-G2 cells
title_full	Optimization of Murrayafoline A ethanol extraction process from the roots of Glycosmis stenocarpa, and evaluation of its Tumorigenesis inhibition activity on Hep-G2 cells
title_fullStr	Optimization of Murrayafoline A ethanol extraction process from the roots of Glycosmis stenocarpa, and evaluation of its Tumorigenesis inhibition activity on Hep-G2 cells
title_full_unstemmed	Optimization of Murrayafoline A ethanol extraction process from the roots of Glycosmis stenocarpa, and evaluation of its Tumorigenesis inhibition activity on Hep-G2 cells
title_sort	optimization of murrayafoline a ethanol extraction process from the roots of glycosmis stenocarpa, and evaluation of its tumorigenesis inhibition activity on hep-g2 cells
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/7cfc32f2b84f46349ade41bc2817f222
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Optimization of Murrayafoline A ethanol extraction process from the roots of Glycosmis stenocarpa, and evaluation of its Tumorigenesis inhibition activity on Hep-G2 cells

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