Engineering Breast Cancer Cells and hUMSCs Microenvironment in 2D and 3D Scaffolds: A Mechanical Study Approach of Stem Cells in Anticancer Therapy

Cell biomechanics plays a major role as a promising biomarker for early cancer diagnosis and prognosis. In the present study, alterations in modulus of elasticity, cell membrane roughness, and migratory potential of MCF-7 (ER+) and SKBR-3 (HER2+) cancer cells were elucidated prior to and post treatm...

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Autores principales:	Despoina Nektaria Metsiou, Foteini K. Kozaniti, Despina D. Deligianni
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cancer stem cells therapy biomechanics dynamic cell culture bioreactor Technology T Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7cfe8c6aa9c1408bb6b295f57628f511
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spelling	oai:doaj.org-article:7cfe8c6aa9c1408bb6b295f57628f5112021-11-25T16:46:43ZEngineering Breast Cancer Cells and hUMSCs Microenvironment in 2D and 3D Scaffolds: A Mechanical Study Approach of Stem Cells in Anticancer Therapy10.3390/bioengineering81101892306-5354https://doaj.org/article/7cfe8c6aa9c1408bb6b295f57628f5112021-11-01T00:00:00Zhttps://www.mdpi.com/2306-5354/8/11/189https://doaj.org/toc/2306-5354Cell biomechanics plays a major role as a promising biomarker for early cancer diagnosis and prognosis. In the present study, alterations in modulus of elasticity, cell membrane roughness, and migratory potential of MCF-7 (ER+) and SKBR-3 (HER2+) cancer cells were elucidated prior to and post treatment with conditioned medium from human umbilical mesenchymal stem cells (hUMSCs-CM) during static and dynamic cell culture. Moreover, the therapeutic potency of hUMSCs-CM on cancer cell’s viability, migratory potential, and F-actin quantified intensity was addressed in 2D surfaces and 3D scaffolds. Interestingly, alterations in ER+ cancer cells showed a positive effect of treatment upon limiting cell viability, motility, and potential for migration. Moreover, increased post treatment cell stiffness indicated rigid cancer cells with confined cell movement and cytoskeletal alterations with restricted lamellipodia formation, which enhanced these results. On the contrary, the cell viability and the migratory potential were not confined post treatment with hUMSCs-CM on HER2+ cells, possibly due to their intrinsic aggressiveness. The increased post treatment cell viability and the decreased cell stiffness indicated an increased potency for cell movement. Hence, the therapy had no efficacy on HER2+ cells.Despoina Nektaria MetsiouFoteini K. KozanitiDespina D. DeligianniMDPI AGarticlecancerstem cellstherapybiomechanicsdynamic cell culturebioreactorTechnologyTBiology (General)QH301-705.5ENBioengineering, Vol 8, Iss 189, p 189 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cancer stem cells therapy biomechanics dynamic cell culture bioreactor Technology T Biology (General) QH301-705.5
spellingShingle	cancer stem cells therapy biomechanics dynamic cell culture bioreactor Technology T Biology (General) QH301-705.5 Despoina Nektaria Metsiou Foteini K. Kozaniti Despina D. Deligianni Engineering Breast Cancer Cells and hUMSCs Microenvironment in 2D and 3D Scaffolds: A Mechanical Study Approach of Stem Cells in Anticancer Therapy
description	Cell biomechanics plays a major role as a promising biomarker for early cancer diagnosis and prognosis. In the present study, alterations in modulus of elasticity, cell membrane roughness, and migratory potential of MCF-7 (ER+) and SKBR-3 (HER2+) cancer cells were elucidated prior to and post treatment with conditioned medium from human umbilical mesenchymal stem cells (hUMSCs-CM) during static and dynamic cell culture. Moreover, the therapeutic potency of hUMSCs-CM on cancer cell’s viability, migratory potential, and F-actin quantified intensity was addressed in 2D surfaces and 3D scaffolds. Interestingly, alterations in ER+ cancer cells showed a positive effect of treatment upon limiting cell viability, motility, and potential for migration. Moreover, increased post treatment cell stiffness indicated rigid cancer cells with confined cell movement and cytoskeletal alterations with restricted lamellipodia formation, which enhanced these results. On the contrary, the cell viability and the migratory potential were not confined post treatment with hUMSCs-CM on HER2+ cells, possibly due to their intrinsic aggressiveness. The increased post treatment cell viability and the decreased cell stiffness indicated an increased potency for cell movement. Hence, the therapy had no efficacy on HER2+ cells.
format	article
author	Despoina Nektaria Metsiou Foteini K. Kozaniti Despina D. Deligianni
author_facet	Despoina Nektaria Metsiou Foteini K. Kozaniti Despina D. Deligianni
author_sort	Despoina Nektaria Metsiou
title	Engineering Breast Cancer Cells and hUMSCs Microenvironment in 2D and 3D Scaffolds: A Mechanical Study Approach of Stem Cells in Anticancer Therapy
title_short	Engineering Breast Cancer Cells and hUMSCs Microenvironment in 2D and 3D Scaffolds: A Mechanical Study Approach of Stem Cells in Anticancer Therapy
title_full	Engineering Breast Cancer Cells and hUMSCs Microenvironment in 2D and 3D Scaffolds: A Mechanical Study Approach of Stem Cells in Anticancer Therapy
title_fullStr	Engineering Breast Cancer Cells and hUMSCs Microenvironment in 2D and 3D Scaffolds: A Mechanical Study Approach of Stem Cells in Anticancer Therapy
title_full_unstemmed	Engineering Breast Cancer Cells and hUMSCs Microenvironment in 2D and 3D Scaffolds: A Mechanical Study Approach of Stem Cells in Anticancer Therapy
title_sort	engineering breast cancer cells and humscs microenvironment in 2d and 3d scaffolds: a mechanical study approach of stem cells in anticancer therapy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7cfe8c6aa9c1408bb6b295f57628f511
work_keys_str_mv	AT despoinanektariametsiou engineeringbreastcancercellsandhumscsmicroenvironmentin2dand3dscaffoldsamechanicalstudyapproachofstemcellsinanticancertherapy AT foteinikkozaniti engineeringbreastcancercellsandhumscsmicroenvironmentin2dand3dscaffoldsamechanicalstudyapproachofstemcellsinanticancertherapy AT despinaddeligianni engineeringbreastcancercellsandhumscsmicroenvironmentin2dand3dscaffoldsamechanicalstudyapproachofstemcellsinanticancertherapy
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Engineering Breast Cancer Cells and hUMSCs Microenvironment in 2D and 3D Scaffolds: A Mechanical Study Approach of Stem Cells in Anticancer Therapy

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