Repeated electromagnetic field stimulation lowers amyloid-β peptide levels in primary human mixed brain tissue cultures

Abstract Late Onset Alzheimer’s Disease is the most common cause of dementia, characterized by extracellular deposition of plaques primarily of amyloid-β (Aβ) peptide and tangles primarily of hyperphosphorylated tau protein. We present data to suggest a noninvasive strategy to decrease potentially t...

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Autores principales:	Felipe P. Perez, Bryan Maloney, Nipun Chopra, Jorge J. Morisaki, Debomoy K. Lahiri
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7d0206775ab945e6a8c0d089921b69d3
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spelling	oai:doaj.org-article:7d0206775ab945e6a8c0d089921b69d32021-12-02T15:23:07ZRepeated electromagnetic field stimulation lowers amyloid-β peptide levels in primary human mixed brain tissue cultures10.1038/s41598-020-77808-22045-2322https://doaj.org/article/7d0206775ab945e6a8c0d089921b69d32021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77808-2https://doaj.org/toc/2045-2322Abstract Late Onset Alzheimer’s Disease is the most common cause of dementia, characterized by extracellular deposition of plaques primarily of amyloid-β (Aβ) peptide and tangles primarily of hyperphosphorylated tau protein. We present data to suggest a noninvasive strategy to decrease potentially toxic Aβ levels, using repeated electromagnetic field stimulation (REMFS) in primary human brain (PHB) cultures. We examined effects of REMFS on Aβ levels (Aβ40 and Aβ42, that are 40 or 42 amino acid residues in length, respectively) in PHB cultures at different frequencies, powers, and specific absorption rates (SAR). PHB cultures at day in vitro 7 (DIV7) treated with 64 MHz, and 1 hour daily for 14 days (DIV 21) had significantly reduced levels of secreted Aβ40 (p = 001) and Aβ42 (p = 0.029) peptides, compared to untreated cultures. PHB cultures (DIV7) treated at 64 MHz, for 1 or 2 hour during 14 days also produced significantly lower Aβ levels. PHB cultures (DIV28) treated with 64 MHz 1 hour/day during 4 or 8 days produced a similar significant reduction in Aβ40 levels. 0.4 W/kg was the minimum SAR required to produce a biological effect. Exposure did not result in cellular toxicity nor significant changes in secreted Aβ precursor protein-α (sAPPα) levels, suggesting the decrease in Aβ did not likely result from redirection toward the α-secretase pathway. EMF frequency and power used in our work is utilized in human magnetic resonance imaging (MRI, thus suggesting REMFS can be further developed in clinical settings to modulate Aβ deposition.Felipe P. PerezBryan MaloneyNipun ChopraJorge J. MorisakiDebomoy K. LahiriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Felipe P. Perez Bryan Maloney Nipun Chopra Jorge J. Morisaki Debomoy K. Lahiri Repeated electromagnetic field stimulation lowers amyloid-β peptide levels in primary human mixed brain tissue cultures
description	Abstract Late Onset Alzheimer’s Disease is the most common cause of dementia, characterized by extracellular deposition of plaques primarily of amyloid-β (Aβ) peptide and tangles primarily of hyperphosphorylated tau protein. We present data to suggest a noninvasive strategy to decrease potentially toxic Aβ levels, using repeated electromagnetic field stimulation (REMFS) in primary human brain (PHB) cultures. We examined effects of REMFS on Aβ levels (Aβ40 and Aβ42, that are 40 or 42 amino acid residues in length, respectively) in PHB cultures at different frequencies, powers, and specific absorption rates (SAR). PHB cultures at day in vitro 7 (DIV7) treated with 64 MHz, and 1 hour daily for 14 days (DIV 21) had significantly reduced levels of secreted Aβ40 (p = 001) and Aβ42 (p = 0.029) peptides, compared to untreated cultures. PHB cultures (DIV7) treated at 64 MHz, for 1 or 2 hour during 14 days also produced significantly lower Aβ levels. PHB cultures (DIV28) treated with 64 MHz 1 hour/day during 4 or 8 days produced a similar significant reduction in Aβ40 levels. 0.4 W/kg was the minimum SAR required to produce a biological effect. Exposure did not result in cellular toxicity nor significant changes in secreted Aβ precursor protein-α (sAPPα) levels, suggesting the decrease in Aβ did not likely result from redirection toward the α-secretase pathway. EMF frequency and power used in our work is utilized in human magnetic resonance imaging (MRI, thus suggesting REMFS can be further developed in clinical settings to modulate Aβ deposition.
format	article
author	Felipe P. Perez Bryan Maloney Nipun Chopra Jorge J. Morisaki Debomoy K. Lahiri
author_facet	Felipe P. Perez Bryan Maloney Nipun Chopra Jorge J. Morisaki Debomoy K. Lahiri
author_sort	Felipe P. Perez
title	Repeated electromagnetic field stimulation lowers amyloid-β peptide levels in primary human mixed brain tissue cultures
title_short	Repeated electromagnetic field stimulation lowers amyloid-β peptide levels in primary human mixed brain tissue cultures
title_full	Repeated electromagnetic field stimulation lowers amyloid-β peptide levels in primary human mixed brain tissue cultures
title_fullStr	Repeated electromagnetic field stimulation lowers amyloid-β peptide levels in primary human mixed brain tissue cultures
title_full_unstemmed	Repeated electromagnetic field stimulation lowers amyloid-β peptide levels in primary human mixed brain tissue cultures
title_sort	repeated electromagnetic field stimulation lowers amyloid-β peptide levels in primary human mixed brain tissue cultures
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7d0206775ab945e6a8c0d089921b69d3
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Repeated electromagnetic field stimulation lowers amyloid-β peptide levels in primary human mixed brain tissue cultures

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