Molecular identification of coffee (Coffea arabica) pollinator insects in North Sumatra, Indonesia based on designed COI primers

Sitompul FA, Siregar EH, Roesma DI, Dahelmi, Prasetya E. 2018. Molecular identification of coffee (Coffea arabica) pollinator insects in North Sumatra, Indonesia based on designed COI primers. Biodiversitas 19: 1877-1883. Coffee (Coffea arabica L.) is one of the most important economic commodities i...

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Autores principales:	AIDA FITRIANI SITOMPUL, ELIDA HAFNI SIREGAR, DEWI IMELDA ROESMA, DAHELMI DAHELMI, EKO PRASETYA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	coffea arabica cytochrome oxidase subunit i mtdna north sumatra pollinator insects Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7d0aaecb38bb4a80b4ac55779da0c4e7
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spelling	oai:doaj.org-article:7d0aaecb38bb4a80b4ac55779da0c4e72021-11-16T14:04:05ZMolecular identification of coffee (Coffea arabica) pollinator insects in North Sumatra, Indonesia based on designed COI primers1412-033X2085-472210.13057/biodiv/d190539https://doaj.org/article/7d0aaecb38bb4a80b4ac55779da0c4e72018-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2970https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Sitompul FA, Siregar EH, Roesma DI, Dahelmi, Prasetya E. 2018. Molecular identification of coffee (Coffea arabica) pollinator insects in North Sumatra, Indonesia based on designed COI primers. Biodiversitas 19: 1877-1883. Coffee (Coffea arabica L.) is one of the most important economic commodities in the province of North Sumatra, Indonesia. Insects associated with pollination of C. arabica are one of the key factors for successful cultivation of C. arabica, but, the research regarding of these was still limited. The population of coffee plant is scattered across the highlands of Indonesia and the pollination of C. arabica is strongly believed linked to a diverse group of pollinating insects. However, lack of taxonomic identification of insects pollinating these plants has become one of constraints to succeed the cultivation of C. Arabica. This study aimed to analyze types and variations of pollinating insects of C. arabica in the province of North Sumatra, Indonesia, using DNA barcoding. DNA barcoding is now considered an alternative method of molecular identification. Sixteen of C. arabica flower visitors were captured in different planting location in North Sumatra province. Using mtDNA markers, the cytochrome oxidase subunit sequence I (COI), about 12 pollinator insect species were identified based on the COI sequence i.e Amegilla cingulata, Apis dorsata, Apis cerana, Trigona chanchamayoensis, Idiella divisa, Dolichopodidae sp., Allactoneura sp., Stomorhina discolor, Phytomia erratica, Rhiniidae sp., Melipona bicolor, and Hymenoptera sp.AIDA FITRIANI SITOMPULELIDA HAFNI SIREGARDEWI IMELDA ROESMADAHELMI DAHELMIEKO PRASETYAMBI & UNS Soloarticlecoffea arabicacytochrome oxidase subunit imtdnanorth sumatrapollinator insectsBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 5, Pp 1876-1883 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	coffea arabica cytochrome oxidase subunit i mtdna north sumatra pollinator insects Biology (General) QH301-705.5
spellingShingle	coffea arabica cytochrome oxidase subunit i mtdna north sumatra pollinator insects Biology (General) QH301-705.5 AIDA FITRIANI SITOMPUL ELIDA HAFNI SIREGAR DEWI IMELDA ROESMA DAHELMI DAHELMI EKO PRASETYA Molecular identification of coffee (Coffea arabica) pollinator insects in North Sumatra, Indonesia based on designed COI primers
description	Sitompul FA, Siregar EH, Roesma DI, Dahelmi, Prasetya E. 2018. Molecular identification of coffee (Coffea arabica) pollinator insects in North Sumatra, Indonesia based on designed COI primers. Biodiversitas 19: 1877-1883. Coffee (Coffea arabica L.) is one of the most important economic commodities in the province of North Sumatra, Indonesia. Insects associated with pollination of C. arabica are one of the key factors for successful cultivation of C. arabica, but, the research regarding of these was still limited. The population of coffee plant is scattered across the highlands of Indonesia and the pollination of C. arabica is strongly believed linked to a diverse group of pollinating insects. However, lack of taxonomic identification of insects pollinating these plants has become one of constraints to succeed the cultivation of C. Arabica. This study aimed to analyze types and variations of pollinating insects of C. arabica in the province of North Sumatra, Indonesia, using DNA barcoding. DNA barcoding is now considered an alternative method of molecular identification. Sixteen of C. arabica flower visitors were captured in different planting location in North Sumatra province. Using mtDNA markers, the cytochrome oxidase subunit sequence I (COI), about 12 pollinator insect species were identified based on the COI sequence i.e Amegilla cingulata, Apis dorsata, Apis cerana, Trigona chanchamayoensis, Idiella divisa, Dolichopodidae sp., Allactoneura sp., Stomorhina discolor, Phytomia erratica, Rhiniidae sp., Melipona bicolor, and Hymenoptera sp.
format	article
author	AIDA FITRIANI SITOMPUL ELIDA HAFNI SIREGAR DEWI IMELDA ROESMA DAHELMI DAHELMI EKO PRASETYA
author_facet	AIDA FITRIANI SITOMPUL ELIDA HAFNI SIREGAR DEWI IMELDA ROESMA DAHELMI DAHELMI EKO PRASETYA
author_sort	AIDA FITRIANI SITOMPUL
title	Molecular identification of coffee (Coffea arabica) pollinator insects in North Sumatra, Indonesia based on designed COI primers
title_short	Molecular identification of coffee (Coffea arabica) pollinator insects in North Sumatra, Indonesia based on designed COI primers
title_full	Molecular identification of coffee (Coffea arabica) pollinator insects in North Sumatra, Indonesia based on designed COI primers
title_fullStr	Molecular identification of coffee (Coffea arabica) pollinator insects in North Sumatra, Indonesia based on designed COI primers
title_full_unstemmed	Molecular identification of coffee (Coffea arabica) pollinator insects in North Sumatra, Indonesia based on designed COI primers
title_sort	molecular identification of coffee (coffea arabica) pollinator insects in north sumatra, indonesia based on designed coi primers
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/7d0aaecb38bb4a80b4ac55779da0c4e7
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Molecular identification of coffee (Coffea arabica) pollinator insects in North Sumatra, Indonesia based on designed COI primers

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