The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors

Klára Jiráková,1 Monika &Scaron;eneklová,1,2 Daniel Jirák,3,4 Karolína Turnovcová,1 Magda Vosmanská,5 Michal Babič,6 Daniel Horák,6 Pavel Veverka,7 Pavla Jendelová1,2 1Department of Neu...

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Autores principales:	Jiráková K, Šeneklova M, Jirak D, Turnovcova K, Vosmanska M, Babic M, Horak D, Veverka P, Jendelova P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	neural precursors magnetic resonance imaging cell differentiation superparamagnetic iron oxide nanoparticles ferrites Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7d0e597f65f64be1ac47bef112861d50
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spelling	oai:doaj.org-article:7d0e597f65f64be1ac47bef112861d502021-12-02T05:00:27ZThe effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors1178-2013https://doaj.org/article/7d0e597f65f64be1ac47bef112861d502016-11-01T00:00:00Zhttps://www.dovepress.com/the-effect-of-magnetic-nanoparticles-on-neuronal-differentiation-of-in-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Klára Jiráková,1 Monika &Scaron;eneklová,1,2 Daniel Jirák,3,4 Karolína Turnovcová,1 Magda Vosmanská,5 Michal Babič,6 Daniel Horák,6 Pavel Veverka,7 Pavla Jendelová1,2 1Department of Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, 2Department of Neuroscience, Second Faculty of Medicine, Charles University, 3MR-Unit, Radiodiagnostic and Interventional Radiology Department, Institute for Clinical and Experimental Medicine, 4Department of Biophysics, Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University, 5Department of Analytical Chemistry, University of Chemistry and Technology, 6Department of Polymer Particles, Institute of Macromolecular Chemistry, 7Department of Magnetics and Superconductors, Institute of Physics, ASCR, Prague, Czech Republic Introduction: Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic nanoparticles (PLL-coated γ-Fe2O3) and studied their effect on proliferation and neuronal differentiation. Materials and methods: We investigated the effect of these two contrast agents on neural precursor cell proliferation and differentiation capability. We further defined the intracellular localization and labeling efficiency and analyzed labeled cells by MR. Results: Cell proliferation was not affected by PLL-coated γ-Fe2O3 but was slowed down in cells labeled with CZF. Labeling efficiency, iron content and relaxation rates measured by MR were lower in cells labeled with CZF when compared to PLL-coated γ-Fe2O3. Cytoplasmic localization of both types of nanoparticles was confirmed by transmission electron microscopy. Flow cytometry and immunocytochemical analysis of specific markers expressed during neuronal differentiation did not show any significant differences between unlabeled cells or cells labeled with both magnetic nanoparticles. Conclusion: Our results show that cells labeled with PLL-coated γ-Fe2O3 are suitable for MR detection, did not affect the differentiation potential of iPSC-NPs and are suitable for in vivo cell therapies in experimental models of central nervous system disorders. Keywords: neural precursors, magnetic resonance imaging, cell differentiation, superparamagnetic iron oxide nanoparticles, ferritesJiráková KŠeneklova MJirak DTurnovcova KVosmanska MBabic MHorak DVeverka PJendelova PDove Medical Pressarticleneural precursorsmagnetic resonance imagingcell differentiationsuperparamagnetic iron oxide nanoparticlesferritesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 6267-6281 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	neural precursors magnetic resonance imaging cell differentiation superparamagnetic iron oxide nanoparticles ferrites Medicine (General) R5-920
spellingShingle	neural precursors magnetic resonance imaging cell differentiation superparamagnetic iron oxide nanoparticles ferrites Medicine (General) R5-920 Jiráková K Šeneklova M Jirak D Turnovcova K Vosmanska M Babic M Horak D Veverka P Jendelova P The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors
description	Klára Jiráková,1 Monika &Scaron;eneklová,1,2 Daniel Jirák,3,4 Karolína Turnovcová,1 Magda Vosmanská,5 Michal Babič,6 Daniel Horák,6 Pavel Veverka,7 Pavla Jendelová1,2 1Department of Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, 2Department of Neuroscience, Second Faculty of Medicine, Charles University, 3MR-Unit, Radiodiagnostic and Interventional Radiology Department, Institute for Clinical and Experimental Medicine, 4Department of Biophysics, Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University, 5Department of Analytical Chemistry, University of Chemistry and Technology, 6Department of Polymer Particles, Institute of Macromolecular Chemistry, 7Department of Magnetics and Superconductors, Institute of Physics, ASCR, Prague, Czech Republic Introduction: Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic nanoparticles (PLL-coated γ-Fe2O3) and studied their effect on proliferation and neuronal differentiation. Materials and methods: We investigated the effect of these two contrast agents on neural precursor cell proliferation and differentiation capability. We further defined the intracellular localization and labeling efficiency and analyzed labeled cells by MR. Results: Cell proliferation was not affected by PLL-coated γ-Fe2O3 but was slowed down in cells labeled with CZF. Labeling efficiency, iron content and relaxation rates measured by MR were lower in cells labeled with CZF when compared to PLL-coated γ-Fe2O3. Cytoplasmic localization of both types of nanoparticles was confirmed by transmission electron microscopy. Flow cytometry and immunocytochemical analysis of specific markers expressed during neuronal differentiation did not show any significant differences between unlabeled cells or cells labeled with both magnetic nanoparticles. Conclusion: Our results show that cells labeled with PLL-coated γ-Fe2O3 are suitable for MR detection, did not affect the differentiation potential of iPSC-NPs and are suitable for in vivo cell therapies in experimental models of central nervous system disorders. Keywords: neural precursors, magnetic resonance imaging, cell differentiation, superparamagnetic iron oxide nanoparticles, ferrites
format	article
author	Jiráková K Šeneklova M Jirak D Turnovcova K Vosmanska M Babic M Horak D Veverka P Jendelova P
author_facet	Jiráková K Šeneklova M Jirak D Turnovcova K Vosmanska M Babic M Horak D Veverka P Jendelova P
author_sort	Jiráková K
title	The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors
title_short	The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors
title_full	The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors
title_fullStr	The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors
title_full_unstemmed	The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors
title_sort	effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/7d0e597f65f64be1ac47bef112861d50
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The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors

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