Identification of a Chromosomal Integrated DNA Fragment Containing the <italic toggle="yes">rmpA2</italic> and <italic toggle="yes">iucABCDiutA</italic> Virulence Genes in <named-content content-type="genus-species">Klebsiella pneumoniae</named-content>

ABSTRACT Klebsiella pneumoniae is increasingly being regarded as a reservoir of diverse antibiotic resistance genes and a pathogen that causes severe infections in both the hospital and the community. In this study, we performed molecular characterization of a carbapenem-resistant and highly virulen...

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Autores principales:	Xuemei Yang, Lianwei Ye, Yi Li, Edward Wai-Chi Chan, Rong Zhang, Sheng Chen
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Klebsiella pneumoniae hypervirulence virulence plasmid chromosome fragment transposition mechanisms Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7d0e7ae326214b4c9663693c23db6c59
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spelling	oai:doaj.org-article:7d0e7ae326214b4c9663693c23db6c592021-11-15T15:31:13ZIdentification of a Chromosomal Integrated DNA Fragment Containing the <italic toggle="yes">rmpA2</italic> and <italic toggle="yes">iucABCDiutA</italic> Virulence Genes in <named-content content-type="genus-species">Klebsiella pneumoniae</named-content>10.1128/mSphere.01179-202379-5042https://doaj.org/article/7d0e7ae326214b4c9663693c23db6c592020-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.01179-20https://doaj.org/toc/2379-5042ABSTRACT Klebsiella pneumoniae is increasingly being regarded as a reservoir of diverse antibiotic resistance genes and a pathogen that causes severe infections in both the hospital and the community. In this study, we performed molecular characterization of a carbapenem-resistant and highly virulent K. pneumoniae strain recovered from a hospital patient. The virulence-encoding genes rmpA2 and iucABCDiutA were found to be located in the chromosome and are flanked by IS26 elements. These chromosomally located virulence genes, if not incurring fitness costs, are expected to be much more stable than plasmid-located genes. Detailed analysis of the fragment in which these virulence genes are located showed that the fragment can readily form a circular intermediate that may promote integration of this virulence-encoding fragment into various plasmid backbones and other chromosomal regions. Findings in this work provide new insights into mechanisms of transmission of virulence-encoding genes in K. pneumoniae. IMPORTANCE This study reported for the first time and characterized in detail the genetic features of a mobile virulence-encoding fragment located in the chromosome of a clinical virulent K. pneumoniae strain and revealed the occurrence of a transposition event mediated by IS26. This genetic structure could mediate the transposition of the virulence-encoding fragment into various plasmid backbones and chromosomes through formation of a circular intermediate. Therefore, findings in this work provide important insights into the transmission mechanisms of mobile virulence profiles in K. pneumoniae strains and lay the foundation for devising effective intervention approaches aimed at preventing the dissemination of these virulence-encoding elements.Xuemei YangLianwei YeYi LiEdward Wai-Chi ChanRong ZhangSheng ChenAmerican Society for MicrobiologyarticleKlebsiella pneumoniaehypervirulencevirulence plasmidchromosome fragmenttransposition mechanismsMicrobiologyQR1-502ENmSphere, Vol 5, Iss 6 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Klebsiella pneumoniae hypervirulence virulence plasmid chromosome fragment transposition mechanisms Microbiology QR1-502
spellingShingle	Klebsiella pneumoniae hypervirulence virulence plasmid chromosome fragment transposition mechanisms Microbiology QR1-502 Xuemei Yang Lianwei Ye Yi Li Edward Wai-Chi Chan Rong Zhang Sheng Chen Identification of a Chromosomal Integrated DNA Fragment Containing the <italic toggle="yes">rmpA2</italic> and <italic toggle="yes">iucABCDiutA</italic> Virulence Genes in <named-content content-type="genus-species">Klebsiella pneumoniae</named-content>
description	ABSTRACT Klebsiella pneumoniae is increasingly being regarded as a reservoir of diverse antibiotic resistance genes and a pathogen that causes severe infections in both the hospital and the community. In this study, we performed molecular characterization of a carbapenem-resistant and highly virulent K. pneumoniae strain recovered from a hospital patient. The virulence-encoding genes rmpA2 and iucABCDiutA were found to be located in the chromosome and are flanked by IS26 elements. These chromosomally located virulence genes, if not incurring fitness costs, are expected to be much more stable than plasmid-located genes. Detailed analysis of the fragment in which these virulence genes are located showed that the fragment can readily form a circular intermediate that may promote integration of this virulence-encoding fragment into various plasmid backbones and other chromosomal regions. Findings in this work provide new insights into mechanisms of transmission of virulence-encoding genes in K. pneumoniae. IMPORTANCE This study reported for the first time and characterized in detail the genetic features of a mobile virulence-encoding fragment located in the chromosome of a clinical virulent K. pneumoniae strain and revealed the occurrence of a transposition event mediated by IS26. This genetic structure could mediate the transposition of the virulence-encoding fragment into various plasmid backbones and chromosomes through formation of a circular intermediate. Therefore, findings in this work provide important insights into the transmission mechanisms of mobile virulence profiles in K. pneumoniae strains and lay the foundation for devising effective intervention approaches aimed at preventing the dissemination of these virulence-encoding elements.
format	article
author	Xuemei Yang Lianwei Ye Yi Li Edward Wai-Chi Chan Rong Zhang Sheng Chen
author_facet	Xuemei Yang Lianwei Ye Yi Li Edward Wai-Chi Chan Rong Zhang Sheng Chen
author_sort	Xuemei Yang
title	Identification of a Chromosomal Integrated DNA Fragment Containing the <italic toggle="yes">rmpA2</italic> and <italic toggle="yes">iucABCDiutA</italic> Virulence Genes in <named-content content-type="genus-species">Klebsiella pneumoniae</named-content>
title_short	Identification of a Chromosomal Integrated DNA Fragment Containing the <italic toggle="yes">rmpA2</italic> and <italic toggle="yes">iucABCDiutA</italic> Virulence Genes in <named-content content-type="genus-species">Klebsiella pneumoniae</named-content>
title_full	Identification of a Chromosomal Integrated DNA Fragment Containing the <italic toggle="yes">rmpA2</italic> and <italic toggle="yes">iucABCDiutA</italic> Virulence Genes in <named-content content-type="genus-species">Klebsiella pneumoniae</named-content>
title_fullStr	Identification of a Chromosomal Integrated DNA Fragment Containing the <italic toggle="yes">rmpA2</italic> and <italic toggle="yes">iucABCDiutA</italic> Virulence Genes in <named-content content-type="genus-species">Klebsiella pneumoniae</named-content>
title_full_unstemmed	Identification of a Chromosomal Integrated DNA Fragment Containing the <italic toggle="yes">rmpA2</italic> and <italic toggle="yes">iucABCDiutA</italic> Virulence Genes in <named-content content-type="genus-species">Klebsiella pneumoniae</named-content>
title_sort	identification of a chromosomal integrated dna fragment containing the <italic toggle="yes">rmpa2</italic> and <italic toggle="yes">iucabcdiuta</italic> virulence genes in <named-content content-type="genus-species">klebsiella pneumoniae</named-content>
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/7d0e7ae326214b4c9663693c23db6c59
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Identification of a Chromosomal Integrated DNA Fragment Containing the <italic toggle="yes">rmpA2</italic> and <italic toggle="yes">iucABCDiutA</italic> Virulence Genes in <named-content content-type="genus-species">Klebsiella pneumoniae</named-content>

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