Comparative study of the antidiabetic potential of Paederia foetida twig extracts and compounds from two different locations in Malaysia

Context: Paederia foetida L. (Rubiaceae) is an edible plant distributed in Asian countries including Malaysia. Fresh leaves have been traditionally used as a remedy for indigestion and diarrhea. Several phytochemical studies of the leaves have been documented, but there are few reports on twigs. Obj...

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Autores principales:	Dai Chuan Tan, Ku Idayu Idris, Nur Kartinee Kassim, Pei Cee Lim, Intan Safinar Ismail, Muhajir Hamid, Rou Chian Ng
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	α-amylase α-glucosidase molecular docking dpph beta-carotene bleaching assay Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/7d0ea6f755e249d1880c873b36751148
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spelling	oai:doaj.org-article:7d0ea6f755e249d1880c873b367511482021-11-17T14:21:56ZComparative study of the antidiabetic potential of Paederia foetida twig extracts and compounds from two different locations in Malaysia1388-02091744-511610.1080/13880209.2019.1610462https://doaj.org/article/7d0ea6f755e249d1880c873b367511482019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1610462https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Paederia foetida L. (Rubiaceae) is an edible plant distributed in Asian countries including Malaysia. Fresh leaves have been traditionally used as a remedy for indigestion and diarrhea. Several phytochemical studies of the leaves have been documented, but there are few reports on twigs. Objective: This study investigates the enzyme inhibition of P. foetida twig extracts and compound isolated from them. In addition, in silico molecular docking of scopoletin was investigated. Materials and methods: Plants were obtained from two locations in Malaysia, Johor (PFJ) and Pahang (PFP). Hexane, chloroform and methanol extracts along with isolated compound (scopoletin) were evaluated for their enzyme inhibition activities (10,000–0.000016 µg/mL). The separation and identification of bio-active compounds were carried out using column chromatography and spectroscopic techniques, respectively. In silico molecular docking of scopoletin with receptors (α-amylase and α-glucosidase) was carried out using AutoDock 4.2. Results: The IC50 values of α-amylase and α-glucosidase inhibition activity of PFJ chloroform extract were 9.60 and 245.6 µg/mL, respectively. PFP chloroform extract exhibited α-amylase and α-glucosidase inhibition activity (IC50 = 14.83 and 257.2 µg/mL, respectively). The α-amylase and α-glucosidase inhibitory activity of scopoletin from both locations had IC50 values of 0.052 and 0.057 µM, respectively. Discussion and conclusions: Separation of PFJ chloroform extract afforded scopoletin (1), stigmasterol (2) and γ-sitosterol (3) and the PFP chloroform extract yielded (1), (2), (3) and ergost-5-en-3-ol (4). Scopoletin was isolated from this species for the first time. In silico calculations gave a binding energy between scopoletin and α-amylase of −6.03 kcal/mol.Dai Chuan TanKu Idayu IdrisNur Kartinee KassimPei Cee LimIntan Safinar IsmailMuhajir HamidRou Chian NgTaylor & Francis Grouparticleα-amylaseα-glucosidasemolecular dockingdpphbeta-carotene bleaching assayTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 345-354 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	α-amylase α-glucosidase molecular docking dpph beta-carotene bleaching assay Therapeutics. Pharmacology RM1-950
spellingShingle	α-amylase α-glucosidase molecular docking dpph beta-carotene bleaching assay Therapeutics. Pharmacology RM1-950 Dai Chuan Tan Ku Idayu Idris Nur Kartinee Kassim Pei Cee Lim Intan Safinar Ismail Muhajir Hamid Rou Chian Ng Comparative study of the antidiabetic potential of Paederia foetida twig extracts and compounds from two different locations in Malaysia
description	Context: Paederia foetida L. (Rubiaceae) is an edible plant distributed in Asian countries including Malaysia. Fresh leaves have been traditionally used as a remedy for indigestion and diarrhea. Several phytochemical studies of the leaves have been documented, but there are few reports on twigs. Objective: This study investigates the enzyme inhibition of P. foetida twig extracts and compound isolated from them. In addition, in silico molecular docking of scopoletin was investigated. Materials and methods: Plants were obtained from two locations in Malaysia, Johor (PFJ) and Pahang (PFP). Hexane, chloroform and methanol extracts along with isolated compound (scopoletin) were evaluated for their enzyme inhibition activities (10,000–0.000016 µg/mL). The separation and identification of bio-active compounds were carried out using column chromatography and spectroscopic techniques, respectively. In silico molecular docking of scopoletin with receptors (α-amylase and α-glucosidase) was carried out using AutoDock 4.2. Results: The IC50 values of α-amylase and α-glucosidase inhibition activity of PFJ chloroform extract were 9.60 and 245.6 µg/mL, respectively. PFP chloroform extract exhibited α-amylase and α-glucosidase inhibition activity (IC50 = 14.83 and 257.2 µg/mL, respectively). The α-amylase and α-glucosidase inhibitory activity of scopoletin from both locations had IC50 values of 0.052 and 0.057 µM, respectively. Discussion and conclusions: Separation of PFJ chloroform extract afforded scopoletin (1), stigmasterol (2) and γ-sitosterol (3) and the PFP chloroform extract yielded (1), (2), (3) and ergost-5-en-3-ol (4). Scopoletin was isolated from this species for the first time. In silico calculations gave a binding energy between scopoletin and α-amylase of −6.03 kcal/mol.
format	article
author	Dai Chuan Tan Ku Idayu Idris Nur Kartinee Kassim Pei Cee Lim Intan Safinar Ismail Muhajir Hamid Rou Chian Ng
author_facet	Dai Chuan Tan Ku Idayu Idris Nur Kartinee Kassim Pei Cee Lim Intan Safinar Ismail Muhajir Hamid Rou Chian Ng
author_sort	Dai Chuan Tan
title	Comparative study of the antidiabetic potential of Paederia foetida twig extracts and compounds from two different locations in Malaysia
title_short	Comparative study of the antidiabetic potential of Paederia foetida twig extracts and compounds from two different locations in Malaysia
title_full	Comparative study of the antidiabetic potential of Paederia foetida twig extracts and compounds from two different locations in Malaysia
title_fullStr	Comparative study of the antidiabetic potential of Paederia foetida twig extracts and compounds from two different locations in Malaysia
title_full_unstemmed	Comparative study of the antidiabetic potential of Paederia foetida twig extracts and compounds from two different locations in Malaysia
title_sort	comparative study of the antidiabetic potential of paederia foetida twig extracts and compounds from two different locations in malaysia
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/7d0ea6f755e249d1880c873b36751148
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Comparative study of the antidiabetic potential of Paederia foetida twig extracts and compounds from two different locations in Malaysia

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