In vitro determination of the efficacy of scorpion venoms as anti-cancer agents against colorectal cancer cells: a nano-liposomal delivery approach

Abdulrahman K Al-Asmari,1 Zabih Ullah,1 Ali Al Balowi,2 Mozaffarul Islam1 1Department of Research, Prince Sultan Military Medical City, Riyadh, 2Department of Pharmacy, King Fahad Armed Forces Hospital, Jeddah, Saudi Arabia Abstract: The use of liposomes in biological and medicinal sciences is a r...

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Autores principales:	Al-Asmari AK, Ullah Z, Al Balowi A, Islam M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	liposomes scorpion venom colon carcinoma cells nanomedicine anticancer Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7d1e2f4b07124da8bd5428aa2f417c3e
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spelling	oai:doaj.org-article:7d1e2f4b07124da8bd5428aa2f417c3e2021-12-02T04:28:16ZIn vitro determination of the efficacy of scorpion venoms as anti-cancer agents against colorectal cancer cells: a nano-liposomal delivery approach1178-2013https://doaj.org/article/7d1e2f4b07124da8bd5428aa2f417c3e2017-01-01T00:00:00Zhttps://www.dovepress.com/in-vitro-determination-of-the-efficacy-of-scorpion-venoms-as-anti-canc-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Abdulrahman K Al-Asmari,1 Zabih Ullah,1 Ali Al Balowi,2 Mozaffarul Islam1 1Department of Research, Prince Sultan Military Medical City, Riyadh, 2Department of Pharmacy, King Fahad Armed Forces Hospital, Jeddah, Saudi Arabia Abstract: The use of liposomes in biological and medicinal sciences is a relatively new approach. The liposomal strategy greatly depends on the technological advancement in the formation of vesicles of various sizes and properties. In the current study, we encapsulated the venoms obtained from medically important scorpions such as Androctonus bicolor (AB), Androctonus crassicauda (AC), and Leiurus quinquestriatus (LQ). To begin with, our first and foremost aim was to prepare biocompatible and biodegradable nanovesicles. Additionally, we intended to enhance the anti-cancer potential of these encapsulated venoms. The liposomal venoms were prepared by rehydration and dehydration methods. Morphology, particle size, and size distribution of the liposomes were examined by scanning electron microscope (SEM), transmission electron microscope (TEM), and Zetasizer. We found that the prepared liposomes had a smooth surface and a spherical/ovoid shape and existed mainly as single unilamellar vesicles (SUVs). Furthermore, the liposomal formulation of all three venoms exhibited excellent stability and good encapsulation efficiency (EE). Additionally, the anti-cancer potential of the encapsulated venoms was also evaluated on a colorectal cancer cell line (HCT-8). The venom-loaded liposomes showed elevated anti-cancer properties such as low rate of cell survival, higher reactive oxygen species (ROS) generation, and enhancement in the number of apoptotic cells. In addition to this, cell cycle analysis revealed G0/G1 enrichment upon venom treatment. The effect of treatment was more pronounced when venom–liposome was used as compared to free venom on the HCT-8 cell line. Furthermore, we did not observe any interference of liposomal lipids used in these preparations on the progression of cancer cells. Considering these findings, we can conclude that the encapsulated scorpion venoms exhibit better efficacy and act more vigorously as an anti-cancer agent on the colorectal cancer cell line when compared with their free counterpart. Keywords: liposomes, scorpion venom, colon carcinoma cells, nanomedicine, anti-cancerAl-Asmari AKUllah ZAl Balowi AIslam MDove Medical Pressarticleliposomesscorpion venomcolon carcinoma cellsnanomedicineanticancerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 559-574 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	liposomes scorpion venom colon carcinoma cells nanomedicine anticancer Medicine (General) R5-920
spellingShingle	liposomes scorpion venom colon carcinoma cells nanomedicine anticancer Medicine (General) R5-920 Al-Asmari AK Ullah Z Al Balowi A Islam M In vitro determination of the efficacy of scorpion venoms as anti-cancer agents against colorectal cancer cells: a nano-liposomal delivery approach
description	Abdulrahman K Al-Asmari,1 Zabih Ullah,1 Ali Al Balowi,2 Mozaffarul Islam1 1Department of Research, Prince Sultan Military Medical City, Riyadh, 2Department of Pharmacy, King Fahad Armed Forces Hospital, Jeddah, Saudi Arabia Abstract: The use of liposomes in biological and medicinal sciences is a relatively new approach. The liposomal strategy greatly depends on the technological advancement in the formation of vesicles of various sizes and properties. In the current study, we encapsulated the venoms obtained from medically important scorpions such as Androctonus bicolor (AB), Androctonus crassicauda (AC), and Leiurus quinquestriatus (LQ). To begin with, our first and foremost aim was to prepare biocompatible and biodegradable nanovesicles. Additionally, we intended to enhance the anti-cancer potential of these encapsulated venoms. The liposomal venoms were prepared by rehydration and dehydration methods. Morphology, particle size, and size distribution of the liposomes were examined by scanning electron microscope (SEM), transmission electron microscope (TEM), and Zetasizer. We found that the prepared liposomes had a smooth surface and a spherical/ovoid shape and existed mainly as single unilamellar vesicles (SUVs). Furthermore, the liposomal formulation of all three venoms exhibited excellent stability and good encapsulation efficiency (EE). Additionally, the anti-cancer potential of the encapsulated venoms was also evaluated on a colorectal cancer cell line (HCT-8). The venom-loaded liposomes showed elevated anti-cancer properties such as low rate of cell survival, higher reactive oxygen species (ROS) generation, and enhancement in the number of apoptotic cells. In addition to this, cell cycle analysis revealed G0/G1 enrichment upon venom treatment. The effect of treatment was more pronounced when venom–liposome was used as compared to free venom on the HCT-8 cell line. Furthermore, we did not observe any interference of liposomal lipids used in these preparations on the progression of cancer cells. Considering these findings, we can conclude that the encapsulated scorpion venoms exhibit better efficacy and act more vigorously as an anti-cancer agent on the colorectal cancer cell line when compared with their free counterpart. Keywords: liposomes, scorpion venom, colon carcinoma cells, nanomedicine, anti-cancer
format	article
author	Al-Asmari AK Ullah Z Al Balowi A Islam M
author_facet	Al-Asmari AK Ullah Z Al Balowi A Islam M
author_sort	Al-Asmari AK
title	In vitro determination of the efficacy of scorpion venoms as anti-cancer agents against colorectal cancer cells: a nano-liposomal delivery approach
title_short	In vitro determination of the efficacy of scorpion venoms as anti-cancer agents against colorectal cancer cells: a nano-liposomal delivery approach
title_full	In vitro determination of the efficacy of scorpion venoms as anti-cancer agents against colorectal cancer cells: a nano-liposomal delivery approach
title_fullStr	In vitro determination of the efficacy of scorpion venoms as anti-cancer agents against colorectal cancer cells: a nano-liposomal delivery approach
title_full_unstemmed	In vitro determination of the efficacy of scorpion venoms as anti-cancer agents against colorectal cancer cells: a nano-liposomal delivery approach
title_sort	in vitro determination of the efficacy of scorpion venoms as anti-cancer agents against colorectal cancer cells: a nano-liposomal delivery approach
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/7d1e2f4b07124da8bd5428aa2f417c3e
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In vitro determination of the efficacy of scorpion venoms as anti-cancer agents against colorectal cancer cells: a nano-liposomal delivery approach

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