Profiling of cytosolic and peroxisomal acetyl-CoA metabolism in Saccharomyces cerevisiae.

As a key intracellular metabolite, acetyl-coenzyme A (acetyl-CoA) plays a major role in various metabolic pathways that link anabolism and catabolism. In the yeast Saccharomyces cerevisiae, acetyl-CoA involving metabolism is compartmentalized, and may vary with the nutrient supply of a cell. Membran...

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Autores principales:	Yun Chen, Verena Siewers, Jens Nielsen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7d2359c100c3440cbfe3c069393cb8ce
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id	oai:doaj.org-article:7d2359c100c3440cbfe3c069393cb8ce
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spelling	oai:doaj.org-article:7d2359c100c3440cbfe3c069393cb8ce2021-11-18T07:09:55ZProfiling of cytosolic and peroxisomal acetyl-CoA metabolism in Saccharomyces cerevisiae.1932-620310.1371/journal.pone.0042475https://doaj.org/article/7d2359c100c3440cbfe3c069393cb8ce2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22876324/?tool=EBIhttps://doaj.org/toc/1932-6203As a key intracellular metabolite, acetyl-coenzyme A (acetyl-CoA) plays a major role in various metabolic pathways that link anabolism and catabolism. In the yeast Saccharomyces cerevisiae, acetyl-CoA involving metabolism is compartmentalized, and may vary with the nutrient supply of a cell. Membranes separating intracellular compartments are impermeable to acetyl-CoA and no direct transport between the compartments occurs. Thus, without carnitine supply the glyoxylate shunt is the sole possible route for transferring acetyl-CoA from the cytosol or the peroxisomes into the mitochondria. Here, we investigate the physiological profiling of different deletion mutants of ACS1, ACS2, CIT2 and MLS1 individually or in combination under alternative carbon sources, and study how various mutations alter carbon distribution. Based on our results a detailed model of carbon distribution about cytosolic and peroxisomal acetyl-CoA metabolism in yeast is suggested. This will be useful to further develop yeast as a cell factory for the biosynthesis of acetyl-CoA-derived products.Yun ChenVerena SiewersJens NielsenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42475 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yun Chen Verena Siewers Jens Nielsen Profiling of cytosolic and peroxisomal acetyl-CoA metabolism in Saccharomyces cerevisiae.
description	As a key intracellular metabolite, acetyl-coenzyme A (acetyl-CoA) plays a major role in various metabolic pathways that link anabolism and catabolism. In the yeast Saccharomyces cerevisiae, acetyl-CoA involving metabolism is compartmentalized, and may vary with the nutrient supply of a cell. Membranes separating intracellular compartments are impermeable to acetyl-CoA and no direct transport between the compartments occurs. Thus, without carnitine supply the glyoxylate shunt is the sole possible route for transferring acetyl-CoA from the cytosol or the peroxisomes into the mitochondria. Here, we investigate the physiological profiling of different deletion mutants of ACS1, ACS2, CIT2 and MLS1 individually or in combination under alternative carbon sources, and study how various mutations alter carbon distribution. Based on our results a detailed model of carbon distribution about cytosolic and peroxisomal acetyl-CoA metabolism in yeast is suggested. This will be useful to further develop yeast as a cell factory for the biosynthesis of acetyl-CoA-derived products.
format	article
author	Yun Chen Verena Siewers Jens Nielsen
author_facet	Yun Chen Verena Siewers Jens Nielsen
author_sort	Yun Chen
title	Profiling of cytosolic and peroxisomal acetyl-CoA metabolism in Saccharomyces cerevisiae.
title_short	Profiling of cytosolic and peroxisomal acetyl-CoA metabolism in Saccharomyces cerevisiae.
title_full	Profiling of cytosolic and peroxisomal acetyl-CoA metabolism in Saccharomyces cerevisiae.
title_fullStr	Profiling of cytosolic and peroxisomal acetyl-CoA metabolism in Saccharomyces cerevisiae.
title_full_unstemmed	Profiling of cytosolic and peroxisomal acetyl-CoA metabolism in Saccharomyces cerevisiae.
title_sort	profiling of cytosolic and peroxisomal acetyl-coa metabolism in saccharomyces cerevisiae.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/7d2359c100c3440cbfe3c069393cb8ce
work_keys_str_mv	AT yunchen profilingofcytosolicandperoxisomalacetylcoametabolisminsaccharomycescerevisiae AT verenasiewers profilingofcytosolicandperoxisomalacetylcoametabolisminsaccharomycescerevisiae AT jensnielsen profilingofcytosolicandperoxisomalacetylcoametabolisminsaccharomycescerevisiae
_version_	1718423859985645568

Profiling of cytosolic and peroxisomal acetyl-CoA metabolism in Saccharomyces cerevisiae.

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