PEG3 controls lipogenesis through ACLY.

PEG3 controls lipogenesis through ACLY.

Peg3 (Paternally expressed gene 3) is an imprinted gene encoding a DNA-binding protein that is a well-known transcriptional repressor. Previous studies have shown that the mutant phenotypes of Peg3 are associated with the over-expression of genes involved in lipid metabolism. In the current study, w...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Subash Ghimire, Joomyeong Kim
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2021
Materias:
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/7d2828410a1043a5b7f374086297e239
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:7d2828410a1043a5b7f374086297e239
record_format dspace
spelling oai:doaj.org-article:7d2828410a1043a5b7f374086297e2392021-12-02T20:05:27ZPEG3 controls lipogenesis through ACLY.1932-620310.1371/journal.pone.0252354https://doaj.org/article/7d2828410a1043a5b7f374086297e2392021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252354https://doaj.org/toc/1932-6203Peg3 (Paternally expressed gene 3) is an imprinted gene encoding a DNA-binding protein that is a well-known transcriptional repressor. Previous studies have shown that the mutant phenotypes of Peg3 are associated with the over-expression of genes involved in lipid metabolism. In the current study, we investigated four potential downstream genes of Peg3, which were identified through ChIP-seq data: Acly, Fasn, Idh1, and Hmgcr. In vivo binding of PEG3 to the promoter region of these key genes involved in lipogenesis was subsequently confirmed through individual ChIP experiments. We observed the opposite response of Acly expression levels against the variable gene dosages of Peg3, involving 0x, 1x, and 2x Peg3. This suggests the transcriptional repressor role of Peg3 in the expression levels of Acly. Another set of analyses showed a sex-biased response in the expression levels of Acly, Fasn, and Idh1 against 0x Peg3 with higher levels in female and lower levels in male mammary glands. These results overall highlight that Peg3 may be involved in regulating the expression levels of several key genes in adipogenesis.Subash GhimireJoomyeong KimPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0252354 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Subash Ghimire
Joomyeong Kim
PEG3 controls lipogenesis through ACLY.
description Peg3 (Paternally expressed gene 3) is an imprinted gene encoding a DNA-binding protein that is a well-known transcriptional repressor. Previous studies have shown that the mutant phenotypes of Peg3 are associated with the over-expression of genes involved in lipid metabolism. In the current study, we investigated four potential downstream genes of Peg3, which were identified through ChIP-seq data: Acly, Fasn, Idh1, and Hmgcr. In vivo binding of PEG3 to the promoter region of these key genes involved in lipogenesis was subsequently confirmed through individual ChIP experiments. We observed the opposite response of Acly expression levels against the variable gene dosages of Peg3, involving 0x, 1x, and 2x Peg3. This suggests the transcriptional repressor role of Peg3 in the expression levels of Acly. Another set of analyses showed a sex-biased response in the expression levels of Acly, Fasn, and Idh1 against 0x Peg3 with higher levels in female and lower levels in male mammary glands. These results overall highlight that Peg3 may be involved in regulating the expression levels of several key genes in adipogenesis.
format article
author Subash Ghimire
Joomyeong Kim
author_facet Subash Ghimire
Joomyeong Kim
author_sort Subash Ghimire
title PEG3 controls lipogenesis through ACLY.
title_short PEG3 controls lipogenesis through ACLY.
title_full PEG3 controls lipogenesis through ACLY.
title_fullStr PEG3 controls lipogenesis through ACLY.
title_full_unstemmed PEG3 controls lipogenesis through ACLY.
title_sort peg3 controls lipogenesis through acly.
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doaj.org/article/7d2828410a1043a5b7f374086297e239
work_keys_str_mv AT subashghimire peg3controlslipogenesisthroughacly
AT joomyeongkim peg3controlslipogenesisthroughacly
_version_ 1718375490677374976

Ejemplares similares