Identification of immunogenic proteins of Waddlia chondrophila.

Evidence is growing for a role of Waddlia chondrophila as an agent of adverse pregnancy outcomes in both humans and ruminants. This emerging pathogen, member of the order Chlamydiales, is also implicated in bronchiolitis and lower respiratory tract infections. Until now, the serological diagnosis of...

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Autores principales:	Carole Kebbi-Beghdadi, Julia Lienard, Frederic Uyttebroeck, David Baud, Beat M Riederer, Gilbert Greub
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7d2c0d84b94e4d438d2a982dd9cfe67b
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spelling	oai:doaj.org-article:7d2c0d84b94e4d438d2a982dd9cfe67b2021-11-18T07:31:00ZIdentification of immunogenic proteins of Waddlia chondrophila.1932-620310.1371/journal.pone.0028605https://doaj.org/article/7d2c0d84b94e4d438d2a982dd9cfe67b2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22238579/?tool=EBIhttps://doaj.org/toc/1932-6203Evidence is growing for a role of Waddlia chondrophila as an agent of adverse pregnancy outcomes in both humans and ruminants. This emerging pathogen, member of the order Chlamydiales, is also implicated in bronchiolitis and lower respiratory tract infections. Until now, the serological diagnosis of W. chondrophila infection has mainly relied on manually intensive tests including micro-immunofluorescence and Western blotting. Thus, there is an urgent need to establish reliable high throughput serological assays. Using a combined genomic and proteomic approach, we detected 57 immunogenic proteins of W. chondrophila, of which 17 were analysed by mass spectrometry. Two novel hypothetical proteins, Wim3 and Wim4, were expressed as recombinant proteins in Escherichia coli, purified and used as antigens in an ELISA test. Both proteins were recognized by sera of rabbits immunized with W. chondrophila as well as by human W. chondrophila positive sera but not by rabbit pre-immune sera nor human W. chondrophila negative sera. These results demonstrated that the approach chosen is suitable to identify immunogenic proteins that can be used to develop a serological test. This latter will be a valuable tool to further clarify the pathogenic potential of W. chondrophila.Carole Kebbi-BeghdadiJulia LienardFrederic UyttebroeckDavid BaudBeat M RiedererGilbert GreubPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e28605 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carole Kebbi-Beghdadi Julia Lienard Frederic Uyttebroeck David Baud Beat M Riederer Gilbert Greub Identification of immunogenic proteins of Waddlia chondrophila.
description	Evidence is growing for a role of Waddlia chondrophila as an agent of adverse pregnancy outcomes in both humans and ruminants. This emerging pathogen, member of the order Chlamydiales, is also implicated in bronchiolitis and lower respiratory tract infections. Until now, the serological diagnosis of W. chondrophila infection has mainly relied on manually intensive tests including micro-immunofluorescence and Western blotting. Thus, there is an urgent need to establish reliable high throughput serological assays. Using a combined genomic and proteomic approach, we detected 57 immunogenic proteins of W. chondrophila, of which 17 were analysed by mass spectrometry. Two novel hypothetical proteins, Wim3 and Wim4, were expressed as recombinant proteins in Escherichia coli, purified and used as antigens in an ELISA test. Both proteins were recognized by sera of rabbits immunized with W. chondrophila as well as by human W. chondrophila positive sera but not by rabbit pre-immune sera nor human W. chondrophila negative sera. These results demonstrated that the approach chosen is suitable to identify immunogenic proteins that can be used to develop a serological test. This latter will be a valuable tool to further clarify the pathogenic potential of W. chondrophila.
format	article
author	Carole Kebbi-Beghdadi Julia Lienard Frederic Uyttebroeck David Baud Beat M Riederer Gilbert Greub
author_facet	Carole Kebbi-Beghdadi Julia Lienard Frederic Uyttebroeck David Baud Beat M Riederer Gilbert Greub
author_sort	Carole Kebbi-Beghdadi
title	Identification of immunogenic proteins of Waddlia chondrophila.
title_short	Identification of immunogenic proteins of Waddlia chondrophila.
title_full	Identification of immunogenic proteins of Waddlia chondrophila.
title_fullStr	Identification of immunogenic proteins of Waddlia chondrophila.
title_full_unstemmed	Identification of immunogenic proteins of Waddlia chondrophila.
title_sort	identification of immunogenic proteins of waddlia chondrophila.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/7d2c0d84b94e4d438d2a982dd9cfe67b
work_keys_str_mv	AT carolekebbibeghdadi identificationofimmunogenicproteinsofwaddliachondrophila AT julialienard identificationofimmunogenicproteinsofwaddliachondrophila AT fredericuyttebroeck identificationofimmunogenicproteinsofwaddliachondrophila AT davidbaud identificationofimmunogenicproteinsofwaddliachondrophila AT beatmriederer identificationofimmunogenicproteinsofwaddliachondrophila AT gilbertgreub identificationofimmunogenicproteinsofwaddliachondrophila
_version_	1718423352841863168

Identification of immunogenic proteins of Waddlia chondrophila.

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