Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces

Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces

Ai Wen Tan,1 Lelia Tay,2 Kien Hui Chua,2 Roslina Ahmad,3 Sheikh Ali Akbar,4 Belinda Pingguan-Murphy1 1Department of Biomedical Engineering, University of Malaya, Kuala Lumpur, Malaysia; 2Department of Physiology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur, Malaysia; 3Departm...

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Autores principales: Tan AW, Tay L, Chua KH, Ahmad R, Ali Akbar S, Pingguan-Murphy B
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2014
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/7d2fec3357bb423cbe9b80768fd326fc
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spelling oai:doaj.org-article:7d2fec3357bb423cbe9b80768fd326fc2021-12-02T04:28:16ZProliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces1178-2013https://doaj.org/article/7d2fec3357bb423cbe9b80768fd326fc2014-11-01T00:00:00Zhttp://www.dovepress.com/proliferation-and-stemness-preservation-of-human-adipose-derived-stem--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Ai Wen Tan,1 Lelia Tay,2 Kien Hui Chua,2 Roslina Ahmad,3 Sheikh Ali Akbar,4 Belinda Pingguan-Murphy1 1Department of Biomedical Engineering, University of Malaya, Kuala Lumpur, Malaysia; 2Department of Physiology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur, Malaysia; 3Department of Mechanical Engineering, University of Malaya, Kuala Lumpur, Malaysia; 4Department of Materials Science and Engineering, The Ohio State University, Columbus, OH, USA Abstract: Two important criteria of an ideal biomaterial in the field of stem cells research are to regulate the cell proliferation without the loss of its pluripotency and to direct the differentiation into a specific cell lineage when desired. The present study describes the influence of TiO2 nanofibrous surface structures on the regulation of proliferation and stemness preservation of adipose-derived stem cells (ADSCs). TiO2 nanofiber arrays were produced in situ onto Ti-6Al-4V substrate via a thermal oxidation process and the successful fabrication of these nanostructures was confirmed by field emission scanning electron microscopy (FESEM), energy dispersive spectrometer (EDS), X-ray diffractometer (XRD), and contact angle measurement. ADSCs were seeded on two types of Ti-6Al-4V surfaces (TiO2 nanofibers and flat control), and their morphology, proliferation, and stemness expression were analyzed using FESEM, AlamarBlue assay, flow cytometry, and quantitative real-time polymerase chain reaction (qRT-PCR) after 2 weeks of incubation, respectively. The results show that ADSCs exhibit better adhesion and significantly enhanced proliferation on the TiO2 nanofibrous surfaces compared to the flat control surfaces. The greater proliferation ability of TiO2 nanofibrous surfaces was further confirmed by the results of cell cycle assay. More importantly, TiO2 nanofibrous surfaces significantly upregulate the expressions of stemness markers Sox-2, Nanog3, Rex-1, and Nestin. These results demonstrate that TiO2 nanofibrous surfaces can be used to enhance cell adhesion and proliferation while simultaneously maintaining the stemness of ADSCs, thereby representing a promising approach for their potential application in the field of bone tissue engineering as well as regenerative therapies. Keywords: titania, nanofibers, thermal oxidation, stem cells, pluripotencyTan AWTay LChua KHAhmad RAli Akbar SPingguan-Murphy BDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 5389-5401 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Tan AW
Tay L
Chua KH
Ahmad R
Ali Akbar S
Pingguan-Murphy B
Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces
description Ai Wen Tan,1 Lelia Tay,2 Kien Hui Chua,2 Roslina Ahmad,3 Sheikh Ali Akbar,4 Belinda Pingguan-Murphy1 1Department of Biomedical Engineering, University of Malaya, Kuala Lumpur, Malaysia; 2Department of Physiology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur, Malaysia; 3Department of Mechanical Engineering, University of Malaya, Kuala Lumpur, Malaysia; 4Department of Materials Science and Engineering, The Ohio State University, Columbus, OH, USA Abstract: Two important criteria of an ideal biomaterial in the field of stem cells research are to regulate the cell proliferation without the loss of its pluripotency and to direct the differentiation into a specific cell lineage when desired. The present study describes the influence of TiO2 nanofibrous surface structures on the regulation of proliferation and stemness preservation of adipose-derived stem cells (ADSCs). TiO2 nanofiber arrays were produced in situ onto Ti-6Al-4V substrate via a thermal oxidation process and the successful fabrication of these nanostructures was confirmed by field emission scanning electron microscopy (FESEM), energy dispersive spectrometer (EDS), X-ray diffractometer (XRD), and contact angle measurement. ADSCs were seeded on two types of Ti-6Al-4V surfaces (TiO2 nanofibers and flat control), and their morphology, proliferation, and stemness expression were analyzed using FESEM, AlamarBlue assay, flow cytometry, and quantitative real-time polymerase chain reaction (qRT-PCR) after 2 weeks of incubation, respectively. The results show that ADSCs exhibit better adhesion and significantly enhanced proliferation on the TiO2 nanofibrous surfaces compared to the flat control surfaces. The greater proliferation ability of TiO2 nanofibrous surfaces was further confirmed by the results of cell cycle assay. More importantly, TiO2 nanofibrous surfaces significantly upregulate the expressions of stemness markers Sox-2, Nanog3, Rex-1, and Nestin. These results demonstrate that TiO2 nanofibrous surfaces can be used to enhance cell adhesion and proliferation while simultaneously maintaining the stemness of ADSCs, thereby representing a promising approach for their potential application in the field of bone tissue engineering as well as regenerative therapies. Keywords: titania, nanofibers, thermal oxidation, stem cells, pluripotency
format article
author Tan AW
Tay L
Chua KH
Ahmad R
Ali Akbar S
Pingguan-Murphy B
author_facet Tan AW
Tay L
Chua KH
Ahmad R
Ali Akbar S
Pingguan-Murphy B
author_sort Tan AW
title Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces
title_short Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces
title_full Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces
title_fullStr Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces
title_full_unstemmed Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces
title_sort proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ tio2 nanofibrous surfaces
publisher Dove Medical Press
publishDate 2014
url https://doaj.org/article/7d2fec3357bb423cbe9b80768fd326fc
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