Morpho-molecular identification and first report of Fusarium equiseti in causing chilli wilt from Kashmir (Northern Himalayas)

Abstract Chilli (Capsicum annuum L.) is one of the most significant vegetable and spice crop. Wilt caused by Fusarium Sp. has emerged as a serious problem in chilli production. Internal transcribed spacer (ITS) region is widely used as a DNA barcoding marker to characterize the diversity and composi...

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Autores principales:	Ammarah Hami, Rovidha S. Rasool, Nisar A. Khan, Sheikh Mansoor, Mudasir A. Mir, Nazeer Ahmed, Khalid Z. Masoodi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7d3225fc71fd4c5781ce6acf1f42787c
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spelling	oai:doaj.org-article:7d3225fc71fd4c5781ce6acf1f42787c2021-12-02T12:14:50ZMorpho-molecular identification and first report of Fusarium equiseti in causing chilli wilt from Kashmir (Northern Himalayas)10.1038/s41598-021-82854-52045-2322https://doaj.org/article/7d3225fc71fd4c5781ce6acf1f42787c2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82854-5https://doaj.org/toc/2045-2322Abstract Chilli (Capsicum annuum L.) is one of the most significant vegetable and spice crop. Wilt caused by Fusarium Sp. has emerged as a serious problem in chilli production. Internal transcribed spacer (ITS) region is widely used as a DNA barcoding marker to characterize the diversity and composition of Fusarium communities. ITS regions are heavily used in both molecular methods and ecological studies of fungi, because of its high degree of interspecific variability, conserved primer sites and multiple copy nature in the genome. In the present study we focused on morphological and molecular characterization of pathogen causing chilli wilt. Chilli plants were collected from four districts of Kashmir valley of Himalayan region. Pathogens were isolated from infected root and stem of the plants. Isolated pathogens were subjected to DNA extraction and PCR amplification. The amplified product was sequenced and three different wilt causing fungal isolates were obtained which are reported in the current investigation. In addition to Fusarium oxysporum and Fusarium solani, a new fungal species was found in association with the chilli wilt in Kashmir valley viz., Fusarium equiseti that has never been reported before from this region. The studies were confirmed by pathogenicity test and re-confirmation by DNA barcoding.Ammarah HamiRovidha S. RasoolNisar A. KhanSheikh MansoorMudasir A. MirNazeer AhmedKhalid Z. MasoodiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ammarah Hami Rovidha S. Rasool Nisar A. Khan Sheikh Mansoor Mudasir A. Mir Nazeer Ahmed Khalid Z. Masoodi Morpho-molecular identification and first report of Fusarium equiseti in causing chilli wilt from Kashmir (Northern Himalayas)
description	Abstract Chilli (Capsicum annuum L.) is one of the most significant vegetable and spice crop. Wilt caused by Fusarium Sp. has emerged as a serious problem in chilli production. Internal transcribed spacer (ITS) region is widely used as a DNA barcoding marker to characterize the diversity and composition of Fusarium communities. ITS regions are heavily used in both molecular methods and ecological studies of fungi, because of its high degree of interspecific variability, conserved primer sites and multiple copy nature in the genome. In the present study we focused on morphological and molecular characterization of pathogen causing chilli wilt. Chilli plants were collected from four districts of Kashmir valley of Himalayan region. Pathogens were isolated from infected root and stem of the plants. Isolated pathogens were subjected to DNA extraction and PCR amplification. The amplified product was sequenced and three different wilt causing fungal isolates were obtained which are reported in the current investigation. In addition to Fusarium oxysporum and Fusarium solani, a new fungal species was found in association with the chilli wilt in Kashmir valley viz., Fusarium equiseti that has never been reported before from this region. The studies were confirmed by pathogenicity test and re-confirmation by DNA barcoding.
format	article
author	Ammarah Hami Rovidha S. Rasool Nisar A. Khan Sheikh Mansoor Mudasir A. Mir Nazeer Ahmed Khalid Z. Masoodi
author_facet	Ammarah Hami Rovidha S. Rasool Nisar A. Khan Sheikh Mansoor Mudasir A. Mir Nazeer Ahmed Khalid Z. Masoodi
author_sort	Ammarah Hami
title	Morpho-molecular identification and first report of Fusarium equiseti in causing chilli wilt from Kashmir (Northern Himalayas)
title_short	Morpho-molecular identification and first report of Fusarium equiseti in causing chilli wilt from Kashmir (Northern Himalayas)
title_full	Morpho-molecular identification and first report of Fusarium equiseti in causing chilli wilt from Kashmir (Northern Himalayas)
title_fullStr	Morpho-molecular identification and first report of Fusarium equiseti in causing chilli wilt from Kashmir (Northern Himalayas)
title_full_unstemmed	Morpho-molecular identification and first report of Fusarium equiseti in causing chilli wilt from Kashmir (Northern Himalayas)
title_sort	morpho-molecular identification and first report of fusarium equiseti in causing chilli wilt from kashmir (northern himalayas)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7d3225fc71fd4c5781ce6acf1f42787c
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Morpho-molecular identification and first report of Fusarium equiseti in causing chilli wilt from Kashmir (Northern Himalayas)

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