Memory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset.

An understanding of the immunological footprint of Mycobacterium tuberculosis (MTB) CD4 T cell recognition is still incomplete. Here we report that human Th1 cells specific for MTB are largely contained in a CXCR3(+)CCR6(+) memory subset and highly focused on three broadly immunodominant antigenic i...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cecilia S Lindestam Arlehamn, Anna Gerasimova, Federico Mele, Ryan Henderson, Justine Swann, Jason A Greenbaum, Yohan Kim, John Sidney, Eddie A James, Randy Taplitz, Denise M McKinney, William W Kwok, Howard Grey, Federica Sallusto, Bjoern Peters, Alessandro Sette
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7d339e7bda6e43eab95b487bd676cd5b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7d339e7bda6e43eab95b487bd676cd5b
record_format	dspace
spelling	oai:doaj.org-article:7d339e7bda6e43eab95b487bd676cd5b2021-11-18T06:06:08ZMemory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset.1553-73661553-737410.1371/journal.ppat.1003130https://doaj.org/article/7d339e7bda6e43eab95b487bd676cd5b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23358848/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374An understanding of the immunological footprint of Mycobacterium tuberculosis (MTB) CD4 T cell recognition is still incomplete. Here we report that human Th1 cells specific for MTB are largely contained in a CXCR3(+)CCR6(+) memory subset and highly focused on three broadly immunodominant antigenic islands, all related to bacterial secretion systems. Our results refute the notion that secreted antigens act as a decoy, since both secreted proteins and proteins comprising the secretion system itself are targeted by a fully functional T cell response. In addition, several novel T cell antigens were identified which can be of potential diagnostic use, or as vaccine antigens. These results underline the power of a truly unbiased, genome-wide, analysis of CD4 MTB recognition based on the combined use of epitope predictions, high throughput ELISPOT, and T cell libraries using PBMCs from individuals latently infected with MTB.Cecilia S Lindestam ArlehamnAnna GerasimovaFederico MeleRyan HendersonJustine SwannJason A GreenbaumYohan KimJohn SidneyEddie A JamesRandy TaplitzDenise M McKinneyWilliam W KwokHoward GreyFederica SallustoBjoern PetersAlessandro SettePublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 1, p e1003130 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Cecilia S Lindestam Arlehamn Anna Gerasimova Federico Mele Ryan Henderson Justine Swann Jason A Greenbaum Yohan Kim John Sidney Eddie A James Randy Taplitz Denise M McKinney William W Kwok Howard Grey Federica Sallusto Bjoern Peters Alessandro Sette Memory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset.
description	An understanding of the immunological footprint of Mycobacterium tuberculosis (MTB) CD4 T cell recognition is still incomplete. Here we report that human Th1 cells specific for MTB are largely contained in a CXCR3(+)CCR6(+) memory subset and highly focused on three broadly immunodominant antigenic islands, all related to bacterial secretion systems. Our results refute the notion that secreted antigens act as a decoy, since both secreted proteins and proteins comprising the secretion system itself are targeted by a fully functional T cell response. In addition, several novel T cell antigens were identified which can be of potential diagnostic use, or as vaccine antigens. These results underline the power of a truly unbiased, genome-wide, analysis of CD4 MTB recognition based on the combined use of epitope predictions, high throughput ELISPOT, and T cell libraries using PBMCs from individuals latently infected with MTB.
format	article
author	Cecilia S Lindestam Arlehamn Anna Gerasimova Federico Mele Ryan Henderson Justine Swann Jason A Greenbaum Yohan Kim John Sidney Eddie A James Randy Taplitz Denise M McKinney William W Kwok Howard Grey Federica Sallusto Bjoern Peters Alessandro Sette
author_facet	Cecilia S Lindestam Arlehamn Anna Gerasimova Federico Mele Ryan Henderson Justine Swann Jason A Greenbaum Yohan Kim John Sidney Eddie A James Randy Taplitz Denise M McKinney William W Kwok Howard Grey Federica Sallusto Bjoern Peters Alessandro Sette
author_sort	Cecilia S Lindestam Arlehamn
title	Memory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset.
title_short	Memory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset.
title_full	Memory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset.
title_fullStr	Memory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset.
title_full_unstemmed	Memory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset.
title_sort	memory t cells in latent mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a cxcr3+ccr6+ th1 subset.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/7d339e7bda6e43eab95b487bd676cd5b
work_keys_str_mv	AT ceciliaslindestamarlehamn memorytcellsinlatentmycobacteriumtuberculosisinfectionaredirectedagainstthreeantigenicislandsandlargelycontainedinacxcr3ccr6th1subset AT annagerasimova memorytcellsinlatentmycobacteriumtuberculosisinfectionaredirectedagainstthreeantigenicislandsandlargelycontainedinacxcr3ccr6th1subset AT federicomele memorytcellsinlatentmycobacteriumtuberculosisinfectionaredirectedagainstthreeantigenicislandsandlargelycontainedinacxcr3ccr6th1subset AT ryanhenderson memorytcellsinlatentmycobacteriumtuberculosisinfectionaredirectedagainstthreeantigenicislandsandlargelycontainedinacxcr3ccr6th1subset AT justineswann memorytcellsinlatentmycobacteriumtuberculosisinfectionaredirectedagainstthreeantigenicislandsandlargelycontainedinacxcr3ccr6th1subset AT jasonagreenbaum memorytcellsinlatentmycobacteriumtuberculosisinfectionaredirectedagainstthreeantigenicislandsandlargelycontainedinacxcr3ccr6th1subset AT yohankim memorytcellsinlatentmycobacteriumtuberculosisinfectionaredirectedagainstthreeantigenicislandsandlargelycontainedinacxcr3ccr6th1subset AT johnsidney memorytcellsinlatentmycobacteriumtuberculosisinfectionaredirectedagainstthreeantigenicislandsandlargelycontainedinacxcr3ccr6th1subset AT eddieajames memorytcellsinlatentmycobacteriumtuberculosisinfectionaredirectedagainstthreeantigenicislandsandlargelycontainedinacxcr3ccr6th1subset AT randytaplitz memorytcellsinlatentmycobacteriumtuberculosisinfectionaredirectedagainstthreeantigenicislandsandlargelycontainedinacxcr3ccr6th1subset AT denisemmckinney memorytcellsinlatentmycobacteriumtuberculosisinfectionaredirectedagainstthreeantigenicislandsandlargelycontainedinacxcr3ccr6th1subset AT williamwkwok memorytcellsinlatentmycobacteriumtuberculosisinfectionaredirectedagainstthreeantigenicislandsandlargelycontainedinacxcr3ccr6th1subset AT howardgrey memorytcellsinlatentmycobacteriumtuberculosisinfectionaredirectedagainstthreeantigenicislandsandlargelycontainedinacxcr3ccr6th1subset AT federicasallusto memorytcellsinlatentmycobacteriumtuberculosisinfectionaredirectedagainstthreeantigenicislandsandlargelycontainedinacxcr3ccr6th1subset AT bjoernpeters memorytcellsinlatentmycobacteriumtuberculosisinfectionaredirectedagainstthreeantigenicislandsandlargelycontainedinacxcr3ccr6th1subset AT alessandrosette memorytcellsinlatentmycobacteriumtuberculosisinfectionaredirectedagainstthreeantigenicislandsandlargelycontainedinacxcr3ccr6th1subset
_version_	1718424549401296896

Memory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset.

Ejemplares similares