Simultaneous Two-Photon Voltage or Calcium Imaging and Multi-Channel Local Field Potential Recordings in Barrel Cortex of Awake and Anesthetized Mice

Neuronal population activity, both spontaneous and sensory-evoked, generates propagating waves in cortex. However, high spatiotemporal-resolution mapping of these waves is difficult as calcium imaging, the work horse of current imaging, does not reveal subthreshold activity. Here, we present a platf...

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Autores principales:	Claudia Cecchetto, Stefano Vassanelli, Bernd Kuhn
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	voltage imaging local field potentials ANNINE-6 two-photon microscopy neuroimaging combined approach Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/7d35ff03175645c7b9ddec3e8a4d382c
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spelling	oai:doaj.org-article:7d35ff03175645c7b9ddec3e8a4d382c2021-11-11T15:26:14ZSimultaneous Two-Photon Voltage or Calcium Imaging and Multi-Channel Local Field Potential Recordings in Barrel Cortex of Awake and Anesthetized Mice1662-453X10.3389/fnins.2021.741279https://doaj.org/article/7d35ff03175645c7b9ddec3e8a4d382c2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnins.2021.741279/fullhttps://doaj.org/toc/1662-453XNeuronal population activity, both spontaneous and sensory-evoked, generates propagating waves in cortex. However, high spatiotemporal-resolution mapping of these waves is difficult as calcium imaging, the work horse of current imaging, does not reveal subthreshold activity. Here, we present a platform combining voltage or calcium two-photon imaging with multi-channel local field potential (LFP) recordings in different layers of the barrel cortex from anesthetized and awake head-restrained mice. A chronic cranial window with access port allows injecting a viral vector expressing GCaMP6f or the voltage-sensitive dye (VSD) ANNINE-6plus, as well as entering the brain with a multi-channel neural probe. We present both average spontaneous activity and average evoked signals in response to multi-whisker air-puff stimulations. Time domain analysis shows the dependence of the evoked responses on the cortical layer and on the state of the animal, here separated into anesthetized, awake but resting, and running. The simultaneous data acquisition allows to compare the average membrane depolarization measured with ANNINE-6plus with the amplitude and shape of the LFP recordings. The calcium imaging data connects these data sets to the large existing database of this important second messenger. Interestingly, in the calcium imaging data, we found a few cells which showed a decrease in calcium concentration in response to vibrissa stimulation in awake mice. This system offers a multimodal technique to study the spatiotemporal dynamics of neuronal signals through a 3D architecture in vivo. It will provide novel insights on sensory coding, closing the gap between electrical and optical recordings.Claudia CecchettoClaudia CecchettoStefano VassanelliStefano VassanelliBernd KuhnFrontiers Media S.A.articlevoltage imaginglocal field potentialsANNINE-6two-photon microscopyneuroimagingcombined approachNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Neuroscience, Vol 15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	voltage imaging local field potentials ANNINE-6 two-photon microscopy neuroimaging combined approach Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	voltage imaging local field potentials ANNINE-6 two-photon microscopy neuroimaging combined approach Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Claudia Cecchetto Claudia Cecchetto Stefano Vassanelli Stefano Vassanelli Bernd Kuhn Simultaneous Two-Photon Voltage or Calcium Imaging and Multi-Channel Local Field Potential Recordings in Barrel Cortex of Awake and Anesthetized Mice
description	Neuronal population activity, both spontaneous and sensory-evoked, generates propagating waves in cortex. However, high spatiotemporal-resolution mapping of these waves is difficult as calcium imaging, the work horse of current imaging, does not reveal subthreshold activity. Here, we present a platform combining voltage or calcium two-photon imaging with multi-channel local field potential (LFP) recordings in different layers of the barrel cortex from anesthetized and awake head-restrained mice. A chronic cranial window with access port allows injecting a viral vector expressing GCaMP6f or the voltage-sensitive dye (VSD) ANNINE-6plus, as well as entering the brain with a multi-channel neural probe. We present both average spontaneous activity and average evoked signals in response to multi-whisker air-puff stimulations. Time domain analysis shows the dependence of the evoked responses on the cortical layer and on the state of the animal, here separated into anesthetized, awake but resting, and running. The simultaneous data acquisition allows to compare the average membrane depolarization measured with ANNINE-6plus with the amplitude and shape of the LFP recordings. The calcium imaging data connects these data sets to the large existing database of this important second messenger. Interestingly, in the calcium imaging data, we found a few cells which showed a decrease in calcium concentration in response to vibrissa stimulation in awake mice. This system offers a multimodal technique to study the spatiotemporal dynamics of neuronal signals through a 3D architecture in vivo. It will provide novel insights on sensory coding, closing the gap between electrical and optical recordings.
format	article
author	Claudia Cecchetto Claudia Cecchetto Stefano Vassanelli Stefano Vassanelli Bernd Kuhn
author_facet	Claudia Cecchetto Claudia Cecchetto Stefano Vassanelli Stefano Vassanelli Bernd Kuhn
author_sort	Claudia Cecchetto
title	Simultaneous Two-Photon Voltage or Calcium Imaging and Multi-Channel Local Field Potential Recordings in Barrel Cortex of Awake and Anesthetized Mice
title_short	Simultaneous Two-Photon Voltage or Calcium Imaging and Multi-Channel Local Field Potential Recordings in Barrel Cortex of Awake and Anesthetized Mice
title_full	Simultaneous Two-Photon Voltage or Calcium Imaging and Multi-Channel Local Field Potential Recordings in Barrel Cortex of Awake and Anesthetized Mice
title_fullStr	Simultaneous Two-Photon Voltage or Calcium Imaging and Multi-Channel Local Field Potential Recordings in Barrel Cortex of Awake and Anesthetized Mice
title_full_unstemmed	Simultaneous Two-Photon Voltage or Calcium Imaging and Multi-Channel Local Field Potential Recordings in Barrel Cortex of Awake and Anesthetized Mice
title_sort	simultaneous two-photon voltage or calcium imaging and multi-channel local field potential recordings in barrel cortex of awake and anesthetized mice
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/7d35ff03175645c7b9ddec3e8a4d382c
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Simultaneous Two-Photon Voltage or Calcium Imaging and Multi-Channel Local Field Potential Recordings in Barrel Cortex of Awake and Anesthetized Mice

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