Elucidating nanoscale mechanical properties of diabetic human adipose tissue using atomic force microscopy

Abstract Obesity-related type 2 diabetes (DM) is a major public health concern. Adipose tissue metabolic dysfunction, including fibrosis, plays a central role in DM pathogenesis. Obesity is associated with changes in adipose tissue extracellular matrix (ECM), but the impact of these changes on adipo...

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Autores principales:	J. K. Wenderott, Carmen G. Flesher, Nicki A. Baker, Christopher K. Neeley, Oliver A. Varban, Carey N. Lumeng, Lutfiyya N. Muhammad, Chen Yeh, Peter F. Green, Robert W. O’Rourke
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7d43f4f971fb465e90cbeab3655e3e1f
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spelling	oai:doaj.org-article:7d43f4f971fb465e90cbeab3655e3e1f2021-12-02T16:08:54ZElucidating nanoscale mechanical properties of diabetic human adipose tissue using atomic force microscopy10.1038/s41598-020-77498-w2045-2322https://doaj.org/article/7d43f4f971fb465e90cbeab3655e3e1f2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77498-whttps://doaj.org/toc/2045-2322Abstract Obesity-related type 2 diabetes (DM) is a major public health concern. Adipose tissue metabolic dysfunction, including fibrosis, plays a central role in DM pathogenesis. Obesity is associated with changes in adipose tissue extracellular matrix (ECM), but the impact of these changes on adipose tissue mechanics and their role in metabolic disease is poorly defined. This study utilized atomic force microscopy (AFM) to quantify difference in elasticity between human DM and non-diabetic (NDM) visceral adipose tissue. The mean elastic modulus of DM adipose tissue was twice that of NDM adipose tissue (11.50 kPa vs. 4.48 kPa) to a 95% confidence level, with significant variability in elasticity of DM compared to NDM adipose tissue. Histologic and chemical measures of fibrosis revealed increased hydroxyproline content in DM adipose tissue, but no difference in Sirius Red staining between DM and NDM tissues. These findings support the hypothesis that fibrosis, evidenced by increased elastic modulus, is enhanced in DM adipose tissue, and suggest that measures of tissue mechanics may better resolve disease-specific differences in adipose tissue fibrosis compared with histologic measures. These data demonstrate the power of AFM nanoindentation to probe tissue mechanics, and delineate the impact of metabolic disease on the mechanical properties of adipose tissue.J. K. WenderottCarmen G. FlesherNicki A. BakerChristopher K. NeeleyOliver A. VarbanCarey N. LumengLutfiyya N. MuhammadChen YehPeter F. GreenRobert W. O’RourkeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q J. K. Wenderott Carmen G. Flesher Nicki A. Baker Christopher K. Neeley Oliver A. Varban Carey N. Lumeng Lutfiyya N. Muhammad Chen Yeh Peter F. Green Robert W. O’Rourke Elucidating nanoscale mechanical properties of diabetic human adipose tissue using atomic force microscopy
description	Abstract Obesity-related type 2 diabetes (DM) is a major public health concern. Adipose tissue metabolic dysfunction, including fibrosis, plays a central role in DM pathogenesis. Obesity is associated with changes in adipose tissue extracellular matrix (ECM), but the impact of these changes on adipose tissue mechanics and their role in metabolic disease is poorly defined. This study utilized atomic force microscopy (AFM) to quantify difference in elasticity between human DM and non-diabetic (NDM) visceral adipose tissue. The mean elastic modulus of DM adipose tissue was twice that of NDM adipose tissue (11.50 kPa vs. 4.48 kPa) to a 95% confidence level, with significant variability in elasticity of DM compared to NDM adipose tissue. Histologic and chemical measures of fibrosis revealed increased hydroxyproline content in DM adipose tissue, but no difference in Sirius Red staining between DM and NDM tissues. These findings support the hypothesis that fibrosis, evidenced by increased elastic modulus, is enhanced in DM adipose tissue, and suggest that measures of tissue mechanics may better resolve disease-specific differences in adipose tissue fibrosis compared with histologic measures. These data demonstrate the power of AFM nanoindentation to probe tissue mechanics, and delineate the impact of metabolic disease on the mechanical properties of adipose tissue.
format	article
author	J. K. Wenderott Carmen G. Flesher Nicki A. Baker Christopher K. Neeley Oliver A. Varban Carey N. Lumeng Lutfiyya N. Muhammad Chen Yeh Peter F. Green Robert W. O’Rourke
author_facet	J. K. Wenderott Carmen G. Flesher Nicki A. Baker Christopher K. Neeley Oliver A. Varban Carey N. Lumeng Lutfiyya N. Muhammad Chen Yeh Peter F. Green Robert W. O’Rourke
author_sort	J. K. Wenderott
title	Elucidating nanoscale mechanical properties of diabetic human adipose tissue using atomic force microscopy
title_short	Elucidating nanoscale mechanical properties of diabetic human adipose tissue using atomic force microscopy
title_full	Elucidating nanoscale mechanical properties of diabetic human adipose tissue using atomic force microscopy
title_fullStr	Elucidating nanoscale mechanical properties of diabetic human adipose tissue using atomic force microscopy
title_full_unstemmed	Elucidating nanoscale mechanical properties of diabetic human adipose tissue using atomic force microscopy
title_sort	elucidating nanoscale mechanical properties of diabetic human adipose tissue using atomic force microscopy
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/7d43f4f971fb465e90cbeab3655e3e1f
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Elucidating nanoscale mechanical properties of diabetic human adipose tissue using atomic force microscopy

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