DEVELOPMENT OF A REAL-TIME RT-PCR-BASED TEST PANEL FOR ASSESSMENT OF CYTOKINE PROFILE IN MONONUCLEAR CELLS FROM BLOOD AND SYNOVIAL FLUID IN RHEUMATOID ARTHRITIS

Abstract. An imbalance between pro- and anti-inflammatory cytokine synthesis plays a crucial role in pathogenesis of rheumatoid arthritis (RA), including erosive joint lesions. Differences in cytokine profiles of RA patients are sufficiently investigated. However, possible interrelations between cyt...

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Autores principales:	D. Yu. Trofimov, O. V. Burmenskaya, E. I. Bateneva, L. P. Alexeev, E. L. Nasonov, E. N. Alexandrova, A. A. Novikov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	rt-pcr cytokine profile mononuclear cells rheumatoid arthritis tnfα il-1β il-6 il-8 il-17 il-15 il-10 ifnγ il-4 il-2 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/7d4d8caa6c6641bf8ab4fd5b09a0c612
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spelling	oai:doaj.org-article:7d4d8caa6c6641bf8ab4fd5b09a0c6122021-11-18T08:03:39ZDEVELOPMENT OF A REAL-TIME RT-PCR-BASED TEST PANEL FOR ASSESSMENT OF CYTOKINE PROFILE IN MONONUCLEAR CELLS FROM BLOOD AND SYNOVIAL FLUID IN RHEUMATOID ARTHRITIS1563-06252313-741X10.15789/1563-0625-2008-6-563-570https://doaj.org/article/7d4d8caa6c6641bf8ab4fd5b09a0c6122014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/269https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. An imbalance between pro- and anti-inflammatory cytokine synthesis plays a crucial role in pathogenesis of rheumatoid arthritis (RA), including erosive joint lesions. Differences in cytokine profiles of RA patients are sufficiently investigated. However, possible interrelations between cytokine profile, immune inflammation, RA progression, and the disease prognosis require further investigations. Due to active search for novel targets in anti-cytokine therapy of RA, evaluation of cytokine levels in peripheral blood and synovial joint fluid remain quite relevant. Therefore, novel methods aimed to determine mRNA expressed by cytokine genes are thought to be promising. Our research was intended to develop test systems for quantitative determination of mRNAs for the following cytokines: TNFα, IL-1β, IL-6, IL-8, IL-17, IL-15, IL-10, IFNγ, IL-4, IL-2. Present article concerns specificities of the developed test systems, description of their technical principles, as well as comparative studies of cytokine gene expression in peripheral blood and synovial joint fluid in RA patients. (Med. Immunol., 2008, vol. 10, N 6, pp 563-570).D. Yu. TrofimovO. V. BurmenskayaE. I. BatenevaL. P. AlexeevE. L. NasonovE. N. AlexandrovaA. A. NovikovSPb RAACIarticlert-pcrcytokine profilemononuclear cellsrheumatoid arthritistnfαil-1βil-6il-8il-17il-15il-10ifnγil-4il-2Immunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 10, Iss 6, Pp 563-570 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	rt-pcr cytokine profile mononuclear cells rheumatoid arthritis tnfα il-1β il-6 il-8 il-17 il-15 il-10 ifnγ il-4 il-2 Immunologic diseases. Allergy RC581-607
spellingShingle	rt-pcr cytokine profile mononuclear cells rheumatoid arthritis tnfα il-1β il-6 il-8 il-17 il-15 il-10 ifnγ il-4 il-2 Immunologic diseases. Allergy RC581-607 D. Yu. Trofimov O. V. Burmenskaya E. I. Bateneva L. P. Alexeev E. L. Nasonov E. N. Alexandrova A. A. Novikov DEVELOPMENT OF A REAL-TIME RT-PCR-BASED TEST PANEL FOR ASSESSMENT OF CYTOKINE PROFILE IN MONONUCLEAR CELLS FROM BLOOD AND SYNOVIAL FLUID IN RHEUMATOID ARTHRITIS
description	Abstract. An imbalance between pro- and anti-inflammatory cytokine synthesis plays a crucial role in pathogenesis of rheumatoid arthritis (RA), including erosive joint lesions. Differences in cytokine profiles of RA patients are sufficiently investigated. However, possible interrelations between cytokine profile, immune inflammation, RA progression, and the disease prognosis require further investigations. Due to active search for novel targets in anti-cytokine therapy of RA, evaluation of cytokine levels in peripheral blood and synovial joint fluid remain quite relevant. Therefore, novel methods aimed to determine mRNA expressed by cytokine genes are thought to be promising. Our research was intended to develop test systems for quantitative determination of mRNAs for the following cytokines: TNFα, IL-1β, IL-6, IL-8, IL-17, IL-15, IL-10, IFNγ, IL-4, IL-2. Present article concerns specificities of the developed test systems, description of their technical principles, as well as comparative studies of cytokine gene expression in peripheral blood and synovial joint fluid in RA patients. (Med. Immunol., 2008, vol. 10, N 6, pp 563-570).
format	article
author	D. Yu. Trofimov O. V. Burmenskaya E. I. Bateneva L. P. Alexeev E. L. Nasonov E. N. Alexandrova A. A. Novikov
author_facet	D. Yu. Trofimov O. V. Burmenskaya E. I. Bateneva L. P. Alexeev E. L. Nasonov E. N. Alexandrova A. A. Novikov
author_sort	D. Yu. Trofimov
title	DEVELOPMENT OF A REAL-TIME RT-PCR-BASED TEST PANEL FOR ASSESSMENT OF CYTOKINE PROFILE IN MONONUCLEAR CELLS FROM BLOOD AND SYNOVIAL FLUID IN RHEUMATOID ARTHRITIS
title_short	DEVELOPMENT OF A REAL-TIME RT-PCR-BASED TEST PANEL FOR ASSESSMENT OF CYTOKINE PROFILE IN MONONUCLEAR CELLS FROM BLOOD AND SYNOVIAL FLUID IN RHEUMATOID ARTHRITIS
title_full	DEVELOPMENT OF A REAL-TIME RT-PCR-BASED TEST PANEL FOR ASSESSMENT OF CYTOKINE PROFILE IN MONONUCLEAR CELLS FROM BLOOD AND SYNOVIAL FLUID IN RHEUMATOID ARTHRITIS
title_fullStr	DEVELOPMENT OF A REAL-TIME RT-PCR-BASED TEST PANEL FOR ASSESSMENT OF CYTOKINE PROFILE IN MONONUCLEAR CELLS FROM BLOOD AND SYNOVIAL FLUID IN RHEUMATOID ARTHRITIS
title_full_unstemmed	DEVELOPMENT OF A REAL-TIME RT-PCR-BASED TEST PANEL FOR ASSESSMENT OF CYTOKINE PROFILE IN MONONUCLEAR CELLS FROM BLOOD AND SYNOVIAL FLUID IN RHEUMATOID ARTHRITIS
title_sort	development of a real-time rt-pcr-based test panel for assessment of cytokine profile in mononuclear cells from blood and synovial fluid in rheumatoid arthritis
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/7d4d8caa6c6641bf8ab4fd5b09a0c612
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DEVELOPMENT OF A REAL-TIME RT-PCR-BASED TEST PANEL FOR ASSESSMENT OF CYTOKINE PROFILE IN MONONUCLEAR CELLS FROM BLOOD AND SYNOVIAL FLUID IN RHEUMATOID ARTHRITIS

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