Characteristics of <i>SlCML39</i>, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of <i>Arabidopsis thaliana</i> during Germination and Seedling Growth

Characteristics of <i>SlCML39</i>, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of <i>Arabidopsis thaliana</i> during Germination and Seedling Growth

Calmodulin-like (CML) proteins are primary calcium sensors and function in plant growth and response to stress stimuli. However, so far, the function of plant CML proteins, including tomato, is still unclear. Previously, it was found that a tomato (<i>Solanum lycopersicum</i>) CML, here...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Haidong Ding, Ying Qian, Yifang Fang, Yurong Ji, Jiarong Sheng, Cailin Ge
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
<i>Arabidopsis thaliana</i>
calmodulin-like protein
high temperature
RNA sequencing
<i>SlCML39</i>
tomato
Biology (General)
QH301-705.5
Chemistry
QD1-999
Acceso en línea:https://doaj.org/article/7d50cbc88e4849008742b4f1db2aecf3
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:7d50cbc88e4849008742b4f1db2aecf3
record_format dspace
spelling oai:doaj.org-article:7d50cbc88e4849008742b4f1db2aecf32021-11-11T16:56:31ZCharacteristics of <i>SlCML39</i>, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of <i>Arabidopsis thaliana</i> during Germination and Seedling Growth10.3390/ijms2221114791422-00671661-6596https://doaj.org/article/7d50cbc88e4849008742b4f1db2aecf32021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11479https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Calmodulin-like (CML) proteins are primary calcium sensors and function in plant growth and response to stress stimuli. However, so far, the function of plant CML proteins, including tomato, is still unclear. Previously, it was found that a tomato (<i>Solanum lycopersicum</i>) CML, here named <i>SlCML39</i>, was significantly induced by high temperature (HT) at transcription level, but its biological function is scarce. In this study, the characteristics of <i>SlCML39</i> and its role in HT tolerance were studied. <i>SlCML39</i> encodes a protein of 201 amino acids containing four EF hand motifs. Many cis-acting elements related to plant stress and hormone response appear in the promoter regions of <i>SlCML39</i>. <i>SlCML39</i> is mainly expressed in the root, stem, and leaf and can be regulated by HT, cold, drought, and salt stresses as well as ABA and H<sub>2</sub>O<sub>2</sub>. Furthermore, heterologous overexpression of <i>SlCML39</i> reduces HT tolerance in <i>Arabidopsis thaliana</i> at the germination and seedling growth stages. To better understand the molecular mechanism of <i>SlCML39</i>, the downstream gene network regulated by <i>SlCML39</i> under HT was analyzed by RNA-Seq. Interestingly, we found that many genes involved in stress responses as well as ABA signal pathway are down-regulated in the transgenic seedlings under HT stress, such as <i>KIN1</i>, <i>RD29B</i>, <i>RD26</i>, and <i>MAP3K18</i>. Collectively, these data indicate that <i>SlCML39</i> acts as an important negative regulator in response to HT stress, which might be mediated by the ABA signal pathway.Haidong DingYing QianYifang FangYurong JiJiarong ShengCailin GeMDPI AGarticle<i>Arabidopsis thaliana</i>calmodulin-like proteinhigh temperatureRNA sequencing<i>SlCML39</i>tomatoBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11479, p 11479 (2021)
institution DOAJ
collection DOAJ
language EN
topic <i>Arabidopsis thaliana</i>
calmodulin-like protein
high temperature
RNA sequencing
<i>SlCML39</i>
tomato
Biology (General)
QH301-705.5
Chemistry
QD1-999
spellingShingle <i>Arabidopsis thaliana</i>
calmodulin-like protein
high temperature
RNA sequencing
<i>SlCML39</i>
tomato
Biology (General)
QH301-705.5
Chemistry
QD1-999
Haidong Ding
Ying Qian
Yifang Fang
Yurong Ji
Jiarong Sheng
Cailin Ge
Characteristics of <i>SlCML39</i>, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of <i>Arabidopsis thaliana</i> during Germination and Seedling Growth
description Calmodulin-like (CML) proteins are primary calcium sensors and function in plant growth and response to stress stimuli. However, so far, the function of plant CML proteins, including tomato, is still unclear. Previously, it was found that a tomato (<i>Solanum lycopersicum</i>) CML, here named <i>SlCML39</i>, was significantly induced by high temperature (HT) at transcription level, but its biological function is scarce. In this study, the characteristics of <i>SlCML39</i> and its role in HT tolerance were studied. <i>SlCML39</i> encodes a protein of 201 amino acids containing four EF hand motifs. Many cis-acting elements related to plant stress and hormone response appear in the promoter regions of <i>SlCML39</i>. <i>SlCML39</i> is mainly expressed in the root, stem, and leaf and can be regulated by HT, cold, drought, and salt stresses as well as ABA and H<sub>2</sub>O<sub>2</sub>. Furthermore, heterologous overexpression of <i>SlCML39</i> reduces HT tolerance in <i>Arabidopsis thaliana</i> at the germination and seedling growth stages. To better understand the molecular mechanism of <i>SlCML39</i>, the downstream gene network regulated by <i>SlCML39</i> under HT was analyzed by RNA-Seq. Interestingly, we found that many genes involved in stress responses as well as ABA signal pathway are down-regulated in the transgenic seedlings under HT stress, such as <i>KIN1</i>, <i>RD29B</i>, <i>RD26</i>, and <i>MAP3K18</i>. Collectively, these data indicate that <i>SlCML39</i> acts as an important negative regulator in response to HT stress, which might be mediated by the ABA signal pathway.
format article
author Haidong Ding
Ying Qian
Yifang Fang
Yurong Ji
Jiarong Sheng
Cailin Ge
author_facet Haidong Ding
Ying Qian
Yifang Fang
Yurong Ji
Jiarong Sheng
Cailin Ge
author_sort Haidong Ding
title Characteristics of <i>SlCML39</i>, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of <i>Arabidopsis thaliana</i> during Germination and Seedling Growth
title_short Characteristics of <i>SlCML39</i>, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of <i>Arabidopsis thaliana</i> during Germination and Seedling Growth
title_full Characteristics of <i>SlCML39</i>, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of <i>Arabidopsis thaliana</i> during Germination and Seedling Growth
title_fullStr Characteristics of <i>SlCML39</i>, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of <i>Arabidopsis thaliana</i> during Germination and Seedling Growth
title_full_unstemmed Characteristics of <i>SlCML39</i>, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of <i>Arabidopsis thaliana</i> during Germination and Seedling Growth
title_sort characteristics of <i>slcml39</i>, a tomato calmodulin-like gene, and its negative role in high temperature tolerance of <i>arabidopsis thaliana</i> during germination and seedling growth
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/7d50cbc88e4849008742b4f1db2aecf3
work_keys_str_mv AT haidongding characteristicsofislcml39iatomatocalmodulinlikegeneanditsnegativeroleinhightemperaturetoleranceofiarabidopsisthalianaiduringgerminationandseedlinggrowth
AT yingqian characteristicsofislcml39iatomatocalmodulinlikegeneanditsnegativeroleinhightemperaturetoleranceofiarabidopsisthalianaiduringgerminationandseedlinggrowth
AT yifangfang characteristicsofislcml39iatomatocalmodulinlikegeneanditsnegativeroleinhightemperaturetoleranceofiarabidopsisthalianaiduringgerminationandseedlinggrowth
AT yurongji characteristicsofislcml39iatomatocalmodulinlikegeneanditsnegativeroleinhightemperaturetoleranceofiarabidopsisthalianaiduringgerminationandseedlinggrowth
AT jiarongsheng characteristicsofislcml39iatomatocalmodulinlikegeneanditsnegativeroleinhightemperaturetoleranceofiarabidopsisthalianaiduringgerminationandseedlinggrowth
AT cailinge characteristicsofislcml39iatomatocalmodulinlikegeneanditsnegativeroleinhightemperaturetoleranceofiarabidopsisthalianaiduringgerminationandseedlinggrowth
_version_ 1718432186353319936

Ejemplares similares